{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T03:59:41Z","timestamp":1780718381972,"version":"3.54.1"},"reference-count":0,"publisher":"Biomedical Informatics","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioinformation"],"published-print":{"date-parts":[[2026,5,31]]},"abstract":"<jats:p>Extended-Spectrum \u03b2-Lactamase (ESBL) -producing Escherichia coli pose a major threat in ICU settings due to rising multidrug resistance and limited data on their genetic diversity. Therefore, it is of interest to analyze the molecular epidemiology of 186 non-duplicate ESBL-producing E. coli isolates from ICU patients over 12 months using Polymerase Chain Reaction (PCR) for ESBL genes, phylogenetic grouping, Multilocus Sequence Typing (MLST) and antimicrobial susceptibility testing. The blaCTX-M gene family was predominant (82.3%), with blaCTX-M-15 as the most common variant and 41.9% of isolates carried multiple ESBL genes. Phylogenetic group B2 and high-risk clones such as ST131, ST405 and ST648 were most prevalent, with high co-resistance to multiple antibiotic classes. Thus, we show significant genetic diversity and dominance of high-risk clones, emphasizing the need for molecular surveillance and targeted infection control strategies.<\/jats:p>","DOI":"10.6026\/973206300222724","type":"journal-article","created":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T03:03:16Z","timestamp":1780714996000},"page":"2724-2730","source":"Crossref","is-referenced-by-count":0,"title":["Genetic diversity of ESBL-producing Escherichia coli in ICU settings"],"prefix":"10.6026","volume":"22","author":[{"given":"Aparna","family":"Aparajita","sequence":"first","affiliation":[{"name":"Department of Microbiology, Rajendra Institute of Medical Sciences (RIMS), Ranchi, Jharkhand, India; *Corresponding author"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manoj","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Rajendra Institute of Medical Sciences (RIMS), Ranchi, Jharkhand, India; *Corresponding author"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashok Kumar","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Rajendra Institute of Medical Sciences (RIMS), Ranchi, Jharkhand, India; *Corresponding author"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4070","published-online":{"date-parts":[[2026,5,31]]},"container-title":["Bioinformation"],"original-title":[],"deposited":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T03:03:22Z","timestamp":1780715002000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.bioinformation.net\/022\/973206300222724.htm"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,31]]},"references-count":0,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,5,31]]},"published-print":{"date-parts":[[2026,5,31]]}},"URL":"https:\/\/doi.org\/10.6026\/973206300222724","relation":{},"ISSN":["0973-8894","0973-2063"],"issn-type":[{"value":"0973-8894","type":"print"},{"value":"0973-2063","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,31]]}}}