{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T12:38:38Z","timestamp":1761741518171,"version":"build-2065373602"},"reference-count":19,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,1,20]],"date-time":"2021-01-20T00:00:00Z","timestamp":1611100800000},"content-version":"vor","delay-in-days":19,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Clinics"],"published-print":{"date-parts":[[2021]]},"DOI":"10.6061\/clinics\/2021\/e2284","type":"journal-article","created":{"date-parts":[[2021,1,20]],"date-time":"2021-01-20T08:18:01Z","timestamp":1611130681000},"page":"e2284","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Polymerase chain reaction targeting 16S ribosomal RNA for the diagnosis of bacterial meningitis after neurosurgery"],"prefix":"10.1016","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7681-8090","authenticated-orcid":false,"given":"Lauro Vieira","family":"Perdig\u00e3o Neto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6870-8898","authenticated-orcid":false,"given":"Micheli","family":"Medeiros","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4190-0238","authenticated-orcid":false,"given":"Suzete Cleusa","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0623-1999","authenticated-orcid":false,"given":"Anna Shoko","family":"Nishiya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8758-5472","authenticated-orcid":false,"given":"Denise Brand\u00e3o","family":"de Assis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0221-3814","authenticated-orcid":false,"given":"\u013acaro","family":"Boszczowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1087-752X","authenticated-orcid":false,"given":"Silvia Figueiredo","family":"Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2427-8368","authenticated-orcid":false,"given":"Anna S.","family":"Levin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.6061\/clinics\/2021\/e2284_bib1","first-page":"1","article-title":"Global neurosurgery: the current capacity and deficit in the provision of essential neurosurgical care. Executive Summary of the Global Neurosurgery Initiative at the Program in Global Surgery and Social Change","author":"Dewan","year":"2018","journal-title":"J Neurosurg"},{"issue":"1","key":"10.6061\/clinics\/2021\/e2284_bib2","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1186\/s13054-020-2749-2","article-title":"Surgery for spontaneous intracerebral hemorrhage","volume":"24","author":"de Oliveira Manoel","year":"2020","journal-title":"Crit Care"},{"issue":"7","key":"10.6061\/clinics\/2021\/e2284_bib3","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0217253","article-title":"Risk factors for postoperative meningitis after microsurgery for vestibular schwannoma","volume":"14","author":"Huang","year":"2019","journal-title":"PLoS One"},{"issue":"4","key":"10.6061\/clinics\/2021\/e2284_bib4","doi-asserted-by":"crossref","first-page":"e151","DOI":"10.1055\/s-0036-1593470","article-title":"Aseptic Meningitis with Craniopharyngioma Resection: Consideration after Endoscopic Surgery","volume":"77","author":"Chen","year":"2016","journal-title":"J Neurol Surg Rep"},{"key":"10.6061\/clinics\/2021\/e2284_bib5","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.jocn.2020.04.077","article-title":"Interpretation of lumbar cerebrospinal fluid leukocytosis after cranial surgery: The relevance of aseptic meningitis","volume":"76","author":"Craven","year":"2020","journal-title":"J Clin Neurosci"},{"issue":"12","key":"10.6061\/clinics\/2021\/e2284_bib6","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1086\/518169","article-title":"Evaluation of the management of postoperative aseptic meningitis","volume":"44","author":"Zarrouk","year":"2007","journal-title":"Clin Infect Dis"},{"issue":"9","key":"10.6061\/clinics\/2021\/e2284_bib7","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1016\/j.cmi.2017.05.013","article-title":"Management of post-neurosurgical meningitis: narrative review","volume":"23","author":"Hussein","year":"2017","journal-title":"Clin Microbiol Infect"},{"issue":"2","key":"10.6061\/clinics\/2021\/e2284_bib8","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.path.2020.01.001","article-title":"Molecular and Histologic Diagnosis of Central Nervous System Infections","volume":"13","author":"Solomon","year":"2020","journal-title":"Surg Pathol Clin"},{"key":"10.6061\/clinics\/2021\/e2284_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.idcr.2020.e00826","article-title":"Value of Broad Range 16S Ribosomal RNA Gene PCR \/ Sequencing (Br-PCR) of CSF in the Diagnosis of Bacterial Meningitis","volume":"21","author":"Rogers","year":"2020","journal-title":"IDCases"},{"issue":"Pt 1","key":"10.6061\/clinics\/2021\/e2284_bib10","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1099\/00221287-148-1-257","article-title":"Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set","volume":"148","author":"Nadkarni","year":"2002","journal-title":"Microbiology (Reading)"},{"issue":"2","key":"10.6061\/clinics\/2021\/e2284_bib11","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1227\/01.NEU.0000255526.34956.E4","article-title":"Diagnosis of ventricular drainage-related bacterial meningitis by broad-range real-time polymerase chain reaction","volume":"61","author":"Deutch","year":"2007","journal-title":"Neurosurgery"},{"key":"10.6061\/clinics\/2021\/e2284_bib12","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2019.02935","article-title":"A New Antibiotic-Loaded Sol-Gel Can Prevent Bacterial Prosthetic Joint Infection: From in vitro Studies to an in vivo Model","volume":"10","author":"Aguilera-Correa","year":"2020","journal-title":"Front Microbiol"},{"issue":"1","key":"10.6061\/clinics\/2021\/e2284_bib13","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/s00701-018-3745-z","article-title":"Incidence, depth, and severity of surgical site infections after neurosurgical interventions","volume":"161","author":"Stienen","year":"2019","journal-title":"Acta Neurochir (Wien)"},{"issue":"4","key":"10.6061\/clinics\/2021\/e2284_bib14","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1007\/s15010-014-0590-8","article-title":"Value of multiplex PCR using cerebrospinal fluid for the diagnosis of ventriculostomy-related meningitis in neurosurgery patients","volume":"42","author":"Rath","year":"2014","journal-title":"Infection"},{"issue":"2","key":"10.6061\/clinics\/2021\/e2284_bib15","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0147765","article-title":"Development of Real-Time PCR Methods for the Detection of Bacterial Meningitis Pathogens without DNA Extraction","volume":"11","author":"Vuong","year":"2016","journal-title":"PLoS One"},{"issue":"3","key":"10.6061\/clinics\/2021\/e2284_bib16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/jid.2013.1","article-title":"Polymerase chain reaction","volume":"133","author":"Garibyan","year":"2013","journal-title":"J Invest Dermatol"},{"issue":"3","key":"10.6061\/clinics\/2021\/e2284_bib17","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.diagmicrobio.2003.10.009","article-title":"Central nervous system infections due to Abiotrophia and Granulicatella species: an emerging challenge?","volume":"48","author":"Cerceo","year":"2004","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"Pt 1","key":"10.6061\/clinics\/2021\/e2284_bib18","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1099\/ijs.0.65315-0","article-title":"Variovorax boronicumulans sp. nov., a boron-accumulating bacterium isolated from soil","volume":"58","author":"Miwa","year":"2008","journal-title":"Int J Syst Evol Microbiol"},{"key":"10.6061\/clinics\/2021\/e2284_bib19","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.nmni.2015.06.004","article-title":"Enterococcus cecorum human infection, France","volume":"7","author":"Delaunay","year":"2015","journal-title":"New Microbes New Infect"}],"container-title":["Clinics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1807593222000850?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1807593222000850?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T07:05:13Z","timestamp":1761721513000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1807593222000850"},"secondary":[{"URL":"https:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1807-59322021000100220&lng=en&nrm=iso","label":"SCIELO"},{"URL":"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7811837\/","label":"PMC"},{"URL":"https:\/\/www.clinicsjournal.com\/article\/polymerase-chain-reaction-targeting-16s-ribosomal-rna-for-the-diagnosis-of-bacterial-meningitis-after-neurosurgery\/","label":"CLINICS"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021]]},"references-count":19,"alternative-id":["S1807593222000850"],"URL":"https:\/\/doi.org\/10.6061\/clinics\/2021\/e2284","relation":{},"ISSN":["1807-5932"],"issn-type":[{"type":"print","value":"1807-5932"}],"subject":[],"published":{"date-parts":[[2021]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Polymerase chain reaction targeting 16S ribosomal RNA for the diagnosis of bacterial meningitis after neurosurgery","name":"articletitle","label":"Article Title"},{"value":"Clinics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.6061\/clinics\/2021\/e2284","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2021 CLINICS. Published by Elsevier Spain","name":"copyright","label":"Copyright"}],"article-number":"e2284"}}