{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,15]],"date-time":"2025-08-15T00:46:32Z","timestamp":1755218792455,"version":"3.43.0"},"reference-count":5,"publisher":"Danish Medical Association","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ugeskr L\u00e6ger"],"abstract":"<jats:p>Visceral leishmaniasis in northern parts of Europe is rare and can be difficult to diagnose. The microbiome 16S\/18S-test is based on next-generation sequencing (NGS)-technology and a software program able to species annotate most types of bacteria, fungi, and parasites. We present a case of an 86-year-old male born in Denmark with multiple myeloma diagnosed with visceral leishmaniasis via the 16S\/18S metabarcode test. This case highlights the potential of the test and the importance of obtaining a thorough travel anamnesis, as immunocompromised patients are susceptible to opportunistic infections.<\/jats:p>","DOI":"10.61409\/v08240552","type":"journal-article","created":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T09:25:37Z","timestamp":1746177937000},"page":"1-4","source":"Crossref","is-referenced-by-count":0,"title":["Diagnosing visceral Leishmaniasis using the microbiome 16S\/18S test"],"prefix":"10.61409","volume":"185","author":[{"given":"Kaziwa B.","family":"Rasoul","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrik Vedel","family":"Nielsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne-Luise","family":"Thorsteinsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anders","family":"Porskrog","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"40208","published-online":{"date-parts":[[2025,4,14]]},"reference":[{"key":"ref0","doi-asserted-by":"publisher","unstructured":"Scarpini S, Dondi A, Totaro C et al. Visceral Leishmaniasis: epidemiology, diagnosis, and treatment regimens in different geographical areas with a focus on pediatrics. Microorganisms. 2022;10(10):1887. https:\/\/doi.org\/10.3390\/microorganisms10101887","DOI":"10.3390\/microorganisms10101887"},{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Holmgaard DB, Barnadas C, Mirbarati SH al. Detection and identification of acanthamoeba and other nonviral causes of infectious keratitis in corneal scrapings by real-time PCR and next-generation sequencing-based 16S-18S gene analysis. J Clin Microbiol. 2021;59(2):e02224-20. https:\/\/doi.org\/10.1128\/JCM.02224-20","DOI":"10.1128\/JCM.02224-20"},{"key":"ref2","doi-asserted-by":"publisher","unstructured":"SSI. Mikrobiom 16S\/18S (bakterier, svampe, parasitter) (R-nr. 1008), 2025. https:\/\/www.ssi.dk\/produkter-og-ydelser\/diagnostik\/diagnostiskhaandbog\/m\/1008 (18. jul 2024)","DOI":"10.1038\/s41566-024-01535-7"},{"key":"ref3","doi-asserted-by":"publisher","unstructured":"Kalmi G, Vignon-Pennamen MD, Ram-Wolff C et al. Visceral leishmaniasis in patients with lymphoma: case reports and review of the literature. Medicine (Baltimore). 2020;99(45):E22787. https:\/\/doi.org\/110.1097\/MD.0000000000022787","DOI":"10.1097\/MD.0000000000022787"},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"Bukan KB, Nardo-Marino A, Hagdrup C et al. Haemophagocytic lymphohistiocytosis associated with leishmaniasis reactivation: a potential adverse event to anti-tumour necrosis factor-\u03b1 therapy. Scand J Rheumatol. 2019;48(4):342-343. https:\/\/doi.org\/10.1080\/03009742.2018.1533035","DOI":"10.1080\/03009742.2018.1533035"}],"container-title":["Ugeskrift for L\u00e6ger"],"original-title":[],"deposited":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T12:09:41Z","timestamp":1754482181000},"score":1,"resource":{"primary":{"URL":"https:\/\/ugeskriftet.dk\/videnskab\/V08240552"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,14]]},"references-count":5,"URL":"https:\/\/doi.org\/10.61409\/v08240552","relation":{},"ISSN":["0041-5782","1603-6824"],"issn-type":[{"type":"print","value":"0041-5782"},{"type":"electronic","value":"1603-6824"}],"subject":[],"published":{"date-parts":[[2025,4,14]]}}}