{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T13:43:45Z","timestamp":1769262225236,"version":"3.49.0"},"reference-count":38,"publisher":"e-Century Publishing Corporation","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am J Transl Res"],"DOI":"10.62347\/kmui2028","type":"journal-article","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T03:36:56Z","timestamp":1764128216000},"page":"8506-8524","source":"Crossref","is-referenced-by-count":0,"title":["Enhanced immunogenicity and protective efficacy of a novel bacillus Calmette-Guérin\/Mycobacterium tuberculosis H37Ra fusion vaccine expressing codon-optimized culture filtrate protein 10 in a mouse model"],"prefix":"10.62347","volume":"17","author":[{"given":"Yuqin","family":"Xue","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"48694","published-online":{"date-parts":[[2025]]},"reference":[{"key":"ref0","doi-asserted-by":"publisher","unstructured":"Myllymaki H, Niskanen M, Oksanen KE and Ramet M. Animal models in tuberculosis research - where is the beef? Expert Opin Drug Discov 2015; 10: 871-883.","DOI":"10.1517\/17460441.2015.1049529"},{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Chen Z, Wang T, Du J, Sun L, Wang G, Ni R, An Y, Fan X, Li Y, Mao RGL, Jing W, Shi K, Cheng J, Wang Q, Nie W, Liu H, Liang J and Gong W. Decoding the WHO global tuberculosis report 2024: a critical analysis of global and chinese key data. Zoonoses 2025; 5.","DOI":"10.15212\/ZOONOSES-2024-0061"},{"key":"ref2","doi-asserted-by":"publisher","unstructured":"Moguche AO, Musvosvi M, Penn-Nicholson A, Plumlee CR, Mearns H, Geldenhuys H, Smit E, Abrahams D, Rozot V, Dintwe O, Hoff ST, Kromann I, Ruhwald M, Bang P, Larson RP, Shafiani S, Ma S, Sherman DR, Sette A, Lindestam Arlehamn CS, McKinney DM, Maecker H, Hanekom WA, Hatherill M, Andersen P, Scriba TJ and Urdahl KB. Antigen availability shapes T cell differentiation and function during tuberculosis. Cell Host Microbe 2017; 21: 695-706, e695.","DOI":"10.1016\/j.chom.2017.05.012"},{"key":"ref3","doi-asserted-by":"publisher","unstructured":"Moguche AO, Musvosvi M, Penn-Nicholson A, Plumlee CR, Mearns H, Geldenhuys H, Smit E, Abrahams D, Rozot V, Dintwe O, Hoff ST, Kromann I, Ruhwald M, Bang P, Larson RP, Shafiani S, Ma S, Sherman DR, Sette A, Lindestam Arlehamn CS, McKinney DM, Maecker H, Hanekom WA, Hatherill M, Andersen P, Scriba TJ and Urdahl KB. Antigen availability shapes T cell differentiation and function during tuberculosis. Cell Host Microbe 2017; 21: 695-706, e5.","DOI":"10.1016\/j.chom.2017.05.012"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"2386030","DOI":"10.1080\/07853890.2024.2386030","article-title":"Biomarkers in diagnosing and therapeutic monitoring of tuberculosis: a review","volume":"56","author":"Leo","year":"2024","unstructured":"Leo S, Narasimhan M, Rathinam S and Banerjee A. Biomarkers in diagnosing and therapeutic monitoring of tuberculosis: a review. Ann Med 2024; 56: 2386030.","journal-title":"Ann Med"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1016\/j.tibtech.2004.04.006","article-title":"Codon bias and heterologous protein expression","volume":"22","author":"Gustafsson","year":"2004","unstructured":"Gustafsson C, Govindarajan S and Minshull J. Codon bias and heterologous protein expression. Trends Biotechnol 2004; 22: 346-53.","journal-title":"Trends Biotechnol"},{"key":"ref6","doi-asserted-by":"publisher","unstructured":"Bhavanam S, Rayat GR, Keelan M, Kunimoto D and Drews SJ. Characterization of immune responses of human PBMCs infected with Mycobacterium tuberculosis H37Ra: impact of donor declared BCG vaccination history on immune responses and M. tuberculosis growth. PLoS One 2018; 13: e0203822.","DOI":"10.1371\/journal.pone.0203822"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1016\/j.mcna.2024.12.001","article-title":"Severe tuberculosis","volume":"109","author":"Muzanyi","year":"2025","unstructured":"Muzanyi G and Bark CM. Severe tuberculosis. Med Clin North Am 2025; 109: 641-650.","journal-title":"Med Clin North Am"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"1107","DOI":"10.1016\/j.cmi.2023.07.013","article-title":"Recent advances in the treatment of tuberculosis","volume":"30","author":"Motta","year":"2024","unstructured":"Motta I, Boeree M, Chesov D, Dheda K, Günther G, Horsburgh CR Jr, Kherabi Y, Lange C, Lienhardt C, McIlleron HM, Paton NI, Stagg HR, Thwaites G, Udwadia Z, Van Crevel R, Velásquez GE, Wilkinson RJ and Guglielmetti L; Study group on Mycobacteria (ESGMYC) of the European Society of Clinical Microbiology and Infectious Diseases (ESCMID). Recent advances in the treatment of tuberculosis. Clin Microbiol Infect 2024; 30: 1107-1114.","journal-title":"Clin Microbiol Infect"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/j.coi.2014.06.003","article-title":"Defining features of protective CD4 T cell responses to Mycobacterium tuberculosis","volume":"29","author":"Sakai","year":"2014","unstructured":"Sakai S, Mayer-Barber KD and Barber DL. Defining features of protective CD4 T cell responses to Mycobacterium tuberculosis. Curr Opin Immunol 2014; 29: 137-42.","journal-title":"Curr Opin Immunol"},{"key":"ref10","doi-asserted-by":"publisher","unstructured":"Gengenbacher M, Nieuwenhuizen N, Vogelzang A, Liu H, Kaiser P, Schuerer S, Lazar D, Wagner I, Mollenkopf HJ and Kaufmann SH. Deletion of nuoG from the vaccine candidate mycobacterium bovis BCG ΔureC::hly improves protection against tuberculosis. mBio 2016; 7: e00679-16.","DOI":"10.1128\/mBio.00679-16"},{"key":"ref11","doi-asserted-by":"publisher","unstructured":"Roy A, Eisenhut M, Harris RJ, Rodrigues LC, Sridhar S, Habermann S, Snell L, Mangtani P, Adetifa I, Lalvani A and Abubakar I. Effect of BCG vaccination against mycobacterium tuberculosis infection in children: systematic review and meta-analysis. BMJ 2014; 349: g4643.","DOI":"10.1136\/bmj.g4643"},{"key":"ref12","doi-asserted-by":"publisher","first-page":"2710","DOI":"10.1016\/j.vaccine.2015.03.083","article-title":"Increased protective efficacy of recombinant BCG strains expressing virulence-neutral proteins of the ESX-1 secretion system","volume":"33","author":"Bottai","year":"2015","unstructured":"Bottai D, Frigui W, Clark S, Rayner E, Zelmer A, Andreu N, de Jonge MI, Bancroft GJ, Williams A, Brodin P and Brosch R. Increased protective efficacy of recombinant BCG strains expressing virulence-neutral proteins of the ESX-1 secretion system. Vaccine 2015; 33: 2710-2718.","journal-title":"Vaccine"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"1900655","DOI":"10.1183\/13993003.00655-2019","article-title":"The global prevalence of latent tuberculosis: a systematic review and meta-analysis","volume":"54","author":"Cohen","year":"2019","unstructured":"Cohen A, Mathiasen VD, Schon T and Wejse C. The global prevalence of latent tuberculosis: a systematic review and meta-analysis. Eur Respir J 2019; 54: 1900655.","journal-title":"Eur Respir J"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"107325","DOI":"10.1016\/j.ijid.2024.107325","article-title":"Insights from the 2024 WHO Global Tuberculosis Report - More Comprehensive Action, Innovation, and Investments required for achieving WHO End TB goals","volume":"150","author":"Goletti","year":"2025","unstructured":"Goletti D, Meintjes G, Andrade BB, Zumla A and Shan Lee S. Insights from the 2024 WHO Global Tuberculosis Report - More Comprehensive Action, Innovation, and Investments required for achieving WHO End TB goals. Int J Infect Dis 2025; 150: 107325.","journal-title":"Int J Infect Dis"},{"key":"ref15","doi-asserted-by":"publisher","unstructured":"Kudla G, Lipinski L, Caffin F, Helwak A and Zylicz M. High guanine and cytosine content increases mRNA levels in mammalian cells. PLoS Biol 2006; 4: e180.","DOI":"10.1371\/journal.pbio.0040180"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"2455","DOI":"10.1007\/s12010-021-03539-3","article-title":"Strain improvement of native saccharomyces cerevisiae LN ITCC 8246 strain through protoplast fusion to enhance its xylose uptake","volume":"193","author":"Sharma","year":"2021","unstructured":"Sharma S, Ghoshal C, Arora A, Samar W, Nain L and Paul D. Strain improvement of native saccharomyces cerevisiae LN ITCC 8246 strain through protoplast fusion to enhance its xylose uptake. Appl Biochem Biotechnol 2021; 193: 2455-2469.","journal-title":"Appl Biochem Biotechnol"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"1765","DOI":"10.1038\/s41591-025-03742-3","article-title":"Strategies for shortening tuberculosis therapy","volume":"31","author":"Dartois","year":"2025","unstructured":"Dartois VA, Mizrahi V, Savic RM, Silverman JA, Hermann D and Barry CE 3rd. Strategies for shortening tuberculosis therapy. Nat Med 2025; 31: 1765-1775.","journal-title":"Nat Med"},{"key":"ref18","doi-asserted-by":"publisher","first-page":"16133","DOI":"10.1038\/nmicrobiol.2016.133","article-title":"Suppression of autophagy and antigen presentation by Mycobacterium tuberculosis PE_PGRS47","volume":"1","author":"NK","year":"2016","unstructured":"Saini NK, Baena A, Ng TW, Venkataswamy MM, Kennedy SC, Kunnath-Velayudhan S, Carreño LJ, Xu J, Chan J, Larsen MH, Jacobs WR Jr and Porcelli SA. Suppression of autophagy and antigen presentation by Mycobacterium tuberculosis PE_PGRS47. Nat Microbiol 2016; 1: 16133.","journal-title":"Nat Microbiol"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"119701","DOI":"10.1016\/j.cca.2024.119701","article-title":"Multidrug-resistant tuberculosis","volume":"559","author":"Wulandari","year":"2024","unstructured":"Wulandari DA, Hartati YW, Ibrahim AU, Pitaloka DAE and Irkham. Multidrug-resistant tuberculosis. Clin Chim Acta 2024; 559: 119701.","journal-title":"Clin Chim Acta"},{"key":"ref20","doi-asserted-by":"publisher","first-page":"850","DOI":"10.1016\/S0140-6736(24)02479-6","article-title":"Tuberculosis","volume":"405","author":"Trajman","year":"2025","unstructured":"Trajman A, Campbell JR, Kunor T, Ruslami R, Amanullah F, Behr MA and Menzies D. Tuberculosis. Lancet 2025; 405: 850-866.","journal-title":"Lancet"},{"key":"ref21","doi-asserted-by":"publisher","first-page":"1797","DOI":"10.1086\/345760","article-title":"kansasii","volume":"186","author":"Arend","year":"2002","unstructured":"Arend SM, van Meijgaarden KE, de Boer K, de Palou EC, van Soolingen D, Ottenhoff TH and van Dissel JT. Tuberculin skin testing and in vitro T cell responses to ESAT-6 and culture filtrate protein 10 after infection with Mycobacterium marinum or M. kansasii. J Infect Dis 2002; 186: 1797-1807.","journal-title":"J Infect Dis"},{"key":"ref22","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1038\/nrd.2017.162","article-title":"Host-directed therapies for bacterial and viral infections","volume":"17","author":"Kaufmann","year":"2018","unstructured":"Kaufmann SHE, Dorhoi A, Hotchkiss RS and Bartenschlager R. Host-directed therapies for bacterial and viral infections. Nat Rev Drug Discov 2018; 17: 35-56.","journal-title":"Nat Rev Drug Discov"},{"key":"ref23","doi-asserted-by":"publisher","first-page":"2081","DOI":"10.1007\/s00256-023-04556-5","article-title":"Imaging of musculoskeletal tuberculosis","volume":"53","author":"Abid","year":"2024","unstructured":"Abid W, Ladeb MF, Chidambaranathan N, Peh WCG and Vanhoenacker FM. Imaging of musculoskeletal tuberculosis. Skeletal Radiol 2024; 53: 2081-2097.","journal-title":"Skeletal Radiol"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1111\/imr.12258","article-title":"Immunology studies in non-human primate models of tuberculosis","volume":"264","author":"Flynn","year":"2015","unstructured":"Flynn JL, Gideon HP, Mattila JT and Lin PL. Immunology studies in non-human primate models of tuberculosis. Immunol Rev 2015; 264: 60-73.","journal-title":"Immunol Rev"},{"key":"ref25","doi-asserted-by":"publisher","unstructured":"Scriba TJ, Maseeme M, Young C, Taylor L and Leslie AJ. Immunopathology in human tuberculosis. Sci Immunol 2024; 9: eado5951.","DOI":"10.1126\/sciimmunol.ado5951"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"100895","DOI":"10.1016\/S2666-5247(24)00127-7","article-title":"Implications of subclinical tuberculosis for vaccine trial design and global effect","volume":"5","author":"Churchyard","year":"2024","unstructured":"Churchyard GJ, Houben RMGJ, Fielding K, Fiore-Gartland AL, Esmail H, Grant AD, Rangaka MX, Behr M, Garcia-Basteiro AL, Wong EB, Hatherill M, Mave V, Dagnew AF, Schmidt AC, Hanekom WA, Cobelens F and White RG. Implications of subclinical tuberculosis for vaccine trial design and global effect. Lancet Microbe 2024; 5: 100895.","journal-title":"Lancet Microbe"},{"key":"ref27","doi-asserted-by":"publisher","first-page":"530","DOI":"10.3390\/vaccines12050530","article-title":"Live attenuated vaccines against tuberculosis: targeting the disruption of genes encoding the secretory proteins of mycobacteria","volume":"12","author":"Veerapandian","year":"2024","unstructured":"Veerapandian R, Gadad SS, Jagannath C and Dhandayuthapani S. Live attenuated vaccines against tuberculosis: targeting the disruption of genes encoding the secretory proteins of mycobacteria. Vaccines (Basel) 2024; 12: 530.","journal-title":"Vaccines (Basel)"},{"key":"ref28","doi-asserted-by":"publisher","first-page":"1104","DOI":"10.1038\/ni.3031","article-title":"Molecular regulation of effector and memory T cell differentiation","volume":"15","author":"JT","year":"2014","unstructured":"Chang JT, Wherry EJ and Goldrath AW. Molecular regulation of effector and memory T cell differentiation. Nat Immunol 2014; 15: 1104-15.","journal-title":"Nat Immunol"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"102057","DOI":"10.1016\/j.berh.2025.102057","article-title":"Musculoskeletal manifestations in Tuberculosis","volume":"39","author":"Subramanian","year":"2025","unstructured":"Subramanian R, Prasad S and Mamadapur M. Musculoskeletal manifestations in Tuberculosis. Best Pract Res Clin Rheumatol 2025; 39: 102057.","journal-title":"Best Pract Res Clin Rheumatol"},{"key":"ref30","doi-asserted-by":"publisher","first-page":"1147","DOI":"10.3389\/fimmu.2017.01147","article-title":"The recombinant bacille calmette-guérin vaccine VPM1002: ready for clinical efficacy testing","volume":"8","author":"Nieuwenhuizen","year":"2017","unstructured":"Nieuwenhuizen NE, Kulkarni PS, Shaligram U, Cotton MF, Rentsch CA, Eisele B, Grode L and Kaufmann SHE. The recombinant bacille calmette-guérin vaccine VPM1002: ready for clinical efficacy testing. Front Immunol 2017; 8: 1147.","journal-title":"Front Immunol"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1007\/s10096-005-1377-8","article-title":"Sensitivity of a new commercial enzyme-linked immunospot assay (T SPOT-TB) for diagnosis of tuberculosis in clinical practice","volume":"24","author":"Meier","year":"2005","unstructured":"Meier T, Eulenbruch HP, Wrighton-Smith P, Enders G and Regnath T. Sensitivity of a new commercial enzyme-linked immunospot assay (T SPOT-TB) for diagnosis of tuberculosis in clinical practice. Eur J Clin Microbiol Infect Dis 2005; 24: 529-36.","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"ref32","doi-asserted-by":"publisher","first-page":"117030","DOI":"10.1016\/j.biopha.2024.117030","article-title":"Mycobacterium tuberculosis infection induces a novel type of cell death: ferroptosis","volume":"177","author":"Wang","year":"2024","unstructured":"Wang J, Cao H, Xie Y, Xu Z, Li Y and Luo H. Mycobacterium tuberculosis infection induces a novel type of cell death: ferroptosis. Biomed Pharmacother 2024; 177: 117030.","journal-title":"Biomed Pharmacother"},{"key":"ref33","doi-asserted-by":"publisher","first-page":"1215","DOI":"10.1080\/13543776.2024.2419826","article-title":"Mycobacterium tuberculosis inhibitors: an updated patent review (2021-present)","volume":"34","author":"Benedetta","year":"2024","unstructured":"Benedetta S, Vallini F, Guida M, Tammaro C, Biava M and Poce G. Mycobacterium tuberculosis inhibitors: an updated patent review (2021-present). Expert Opin Ther Pat 2024; 34: 1215-1230.","journal-title":"Expert Opin Ther Pat"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"6658","DOI":"10.1038\/s41467-021-26934-0","article-title":"A mycobacterium tuberculosis-specific subunit vaccine that provides synergistic immunity upon co-administration with Bacillus Calmette-Guérin","volume":"12","author":"JS","year":"2021","unstructured":"Woodworth JS, Clemmensen HS, Battey H, Dijkman K, Lindenstrøm T, Laureano RS, Taplitz R, Morgan J, Aagaard C, Rosenkrands I, Lindestam Arlehamn CS, Andersen P and Mortensen R. A mycobacterium tuberculosis-specific subunit vaccine that provides synergistic immunity upon co-administration with Bacillus Calmette-Guérin. Nat Commun 2021; 12: 6658.","journal-title":"Nat Commun"},{"key":"ref35","doi-asserted-by":"publisher","unstructured":"Mohammadnabi N, Shamseddin J, Emadi M, Bodaghi AB, Varseh M, Shariati A, Rezaei M, Dastranj M and Farahani A. Mycobacterium tuberculosis: the mechanism of pathogenicity, immune responses, and diagnostic challenges. J Clin Lab Anal 2024; 38: e25122.","DOI":"10.1002\/jcla.25122"},{"key":"ref36","doi-asserted-by":"publisher","first-page":"121","DOI":"10.3389\/fimmu.2018.00121","article-title":"Next-generation vaccines based on bacille calmette-guerin","volume":"9","author":"Nieuwenhuizen","year":"2018","unstructured":"Nieuwenhuizen NE and Kaufmann SHE. Next-generation vaccines based on bacille calmette-guerin. Front Immunol 2018; 9: 121.","journal-title":"Front Immunol"},{"key":"ref37","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1016\/j.ijid.2016.10.018","article-title":"Novel approaches to tuberculosis vaccine development","volume":"56","author":"SH","year":"2017","unstructured":"Kaufmann SH, Weiner J and von Reyn CF. Novel approaches to tuberculosis vaccine development. Int J Infect Dis 2017; 56: 263-267.","journal-title":"Int J Infect Dis"}],"container-title":["American Journal of Translational Research"],"original-title":[],"link":[{"URL":"https:\/\/e-century.us\/files\/ajtr\/17\/11\/ajtr0167589.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T06:10:53Z","timestamp":1769148653000},"score":1,"resource":{"primary":{"URL":"https:\/\/e-century.us\/files\/ajtr\/17\/11\/ajtr0167589.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":38,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.62347\/kmui2028","relation":{},"ISSN":["1943-8141"],"issn-type":[{"value":"1943-8141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]}}}