{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T06:13:23Z","timestamp":1770358403184,"version":"3.49.0"},"reference-count":41,"publisher":"e-Century Publishing Corporation","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am J Transl Res"],"DOI":"10.62347\/rqhy5963","type":"journal-article","created":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T03:45:35Z","timestamp":1719459935000},"page":"2190-2211","source":"Crossref","is-referenced-by-count":2,"title":["Integrating network pharmacology with experimental validation to investigate the mechanism of Wuwei Zishen formula in improving perimenopausal syndrome"],"prefix":"10.62347","volume":"16","author":[{"given":"Xuewen","family":"Fu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"48694","published-online":{"date-parts":[[2024]]},"reference":[{"key":"ref0","doi-asserted-by":"publisher","first-page":"20220754","DOI":"10.1515\/biol-2022-0754","article-title":"Advances in diagnosis and treatment of perimenopausal syndrome","volume":"18","author":"Chen","year":"2023","unstructured":"Chen W, Chen M, Tang H, Wei W, Shao P, Dou S, Wu J, Lu B, Shi R and Chen J. Advances in diagnosis and treatment of perimenopausal syndrome. Open Life Sci 2023; 18: 20220754.","journal-title":"Open Life Sci"},{"key":"ref1","doi-asserted-by":"publisher","first-page":"855","DOI":"10.1007\/s00404-021-06139-y","article-title":"Assessment of the climacteric syndrome: a narrative review","volume":"304","author":"Sourouni","year":"2021","unstructured":"Sourouni M, Zangger M, Honermann L, Foth D and Stute P. Assessment of the climacteric syndrome: a narrative review. Arch Gynecol Obstet 2021; 304: 855-862.","journal-title":"Arch Gynecol Obstet"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"332","DOI":"10.1089\/jwh.2015.5556","article-title":"Perimenopause: from research to practice","volume":"25","author":"Santoro","year":"2016","unstructured":"Santoro N. Perimenopause: from research to practice. J Womens Health (Larchmt) 2016; 25: 332-339.","journal-title":"J Womens Health (Larchmt)"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"528","DOI":"10.1097\/GRF.0000000000000639","article-title":"Menopause: a global perspective and clinical guide for practice","volume":"64","author":"Voedisch","year":"2021","unstructured":"Voedisch AJ, Dunsmoor-Su R and Kasirsky J. Menopause: a global perspective and clinical guide for practice. Clin Obstet Gynecol 2021; 64: 528-554.","journal-title":"Clin Obstet Gynecol"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"46","DOI":"10.5582\/bst.2021.01418","article-title":"Update on hormone therapy for the management of postmenopausal women","volume":"16","author":"Pan","year":"2022","unstructured":"Pan M, Pan X, Zhou J, Wang J, Qi Q and Wang L. Update on hormone therapy for the management of postmenopausal women. Biosci Trends 2022; 16: 46-57.","journal-title":"Biosci Trends"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"720","DOI":"10.1210\/endrev\/bnab011","article-title":"Hormone therapy in menopause: concepts, controversies, and approach to treatment","volume":"42","author":"VA","year":"2021","unstructured":"Flores VA, Pal L and Manson JE. Hormone therapy in menopause: concepts, controversies, and approach to treatment. Endocr Rev 2021; 42: 720-752.","journal-title":"Endocr Rev"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1016\/j.jep.2019.01.010","article-title":"Erxian decoction, a Chinese herbal formula, for menopausal syndrome: an updated systematic review","volume":"234","author":"Wang","year":"2019","unstructured":"Wang Y, Lou XT, Shi YH, Tong Q and Zheng GQ. Erxian decoction, a Chinese herbal formula, for menopausal syndrome: an updated systematic review. J Ethnopharmacol 2019; 234: 8-20.","journal-title":"J Ethnopharmacol"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"230","DOI":"10.5694\/j.1326-5377.2001.tb143119.x","article-title":"The effects of Chinese medicinal herbs on postmenopausal vasomotor symptoms of Australian women","volume":"175","author":"Nguyen","year":"2001","unstructured":"Nguyen TV and Wong FW. The effects of Chinese medicinal herbs on postmenopausal vasomotor symptoms of Australian women. Med J Aust 2001; 175: 230.","journal-title":"Med J Aust"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"1193","DOI":"10.1097\/GME.0000000000001380","article-title":"Effectiveness of Chinese herbal medicine granules and traditional Chinese medicine-based psychotherapy for perimenopausal depression in Chinese women: a randomized controlled trial","volume":"26","author":"XJ","year":"2019","unstructured":"Cao XJ, Huang XC and Wang X. Effectiveness of Chinese herbal medicine granules and traditional Chinese medicine-based psychotherapy for perimenopausal depression in Chinese women: a randomized controlled trial. Menopause 2019; 26: 1193-1203.","journal-title":"Menopause"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"115057","DOI":"10.1016\/j.jep.2022.115057","article-title":"Network pharmacology integrated with experimental validation reveals the regulatory mechanism of action of Hehuan Yin decoction in polycystic ovary syndrome with insulin resistance","volume":"289","author":"YY","year":"2022","unstructured":"Wu YY, Li SY, Zhu HQ, Zhuang ZM, Shao M, Chen FL, Liu CS and Tang QF. Network pharmacology integrated with experimental validation reveals the regulatory mechanism of action of Hehuan Yin decoction in polycystic ovary syndrome with insulin resistance. J Ethnopharmacol 2022; 289: 115057.","journal-title":"J Ethnopharmacol"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"317","DOI":"10.14715\/cmb\/2021.67.4.43","article-title":"Effect of Tiaojingzhixue Fang on the expression of sex hormone and endometrial tissue mRNA in perimenopausal patients with abnormal uterine bleeding","volume":"67","author":"Guan","year":"2022","unstructured":"Guan L, Xue L, Chu J, Xue J, Zhang S and Zhu L. Effect of Tiaojingzhixue Fang on the expression of sex hormone and endometrial tissue mRNA in perimenopausal patients with abnormal uterine bleeding. Cell Mol Biol (Noisy-le-grand) 2022; 67: 317-323.","journal-title":"Cell Mol Biol (Noisy-le-grand)"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"574","DOI":"10.1080\/13697137.2016.1238453","article-title":"A randomized, double-blind, placebo-controlled trial of Schisandra chinensis for menopausal symptoms","volume":"19","author":"Park","year":"2016","unstructured":"Park JY and Kim KH. A randomized, double-blind, placebo-controlled trial of Schisandra chinensis for menopausal symptoms. Climacteric 2016; 19: 574-580.","journal-title":"Climacteric"},{"key":"ref12","doi-asserted-by":"publisher","first-page":"109060","DOI":"10.1016\/j.biopha.2019.109060","article-title":"Leonurus japonicus (Chinese motherwort), an excellent traditional medicine for obstetrical and gynecological diseases: a comprehensive overview","volume":"117","author":"Miao","year":"2019","unstructured":"Miao LL, Zhou QM, Peng C, Liu ZH and Xiong L. Leonurus japonicus (Chinese motherwort), an excellent traditional medicine for obstetrical and gynecological diseases: a comprehensive overview. Biomed Pharmacother 2019; 117: 109060.","journal-title":"Biomed Pharmacother"},{"key":"ref13","unstructured":"Pei MR, Meng S and Li HF. Comparative study of antiperspirant effect and content of 5-undecylresorcinol and other compounds between floating wheat and wheat. Chinese Archives of Traditional Chinese Medicine 2013; 31: 2030-2032."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"822","DOI":"10.1080\/10408390902773052","article-title":"Cinnamon and health","volume":"50","author":"Gruenwald","year":"2010","unstructured":"Gruenwald J, Freder J and Armbruester N. Cinnamon and health. Crit Rev Food Sci Nutr 2010; 50: 822-834.","journal-title":"Crit Rev Food Sci Nutr"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"3713","DOI":"10.3390\/nu15173713","article-title":"Combined treatment of mori folium and mori cortex radicis ameliorate obesity in mice via UCP-1 in brown adipocytes","volume":"15","author":"Kim","year":"2023","unstructured":"Kim DS, Lee HY, Kim HJ, Lee GH, Lim YJ, Ko BM, Kim JH, Kim TW, Kim HK, Kim TY, Hwang DI, Choi HK, Ju SM, Chung MJ and Chae HJ. Combined treatment of mori folium and mori cortex radicis ameliorate obesity in mice via UCP-1 in brown adipocytes. Nutrients 2023; 15: 3713.","journal-title":"Nutrients"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1016\/j.jep.2014.09.033","article-title":"Inhibitory effect of Ecliptae herba extract and its component wedelolactone on pre-osteoclastic proliferation and differentiation","volume":"157","author":"Liu","year":"2014","unstructured":"Liu YQ, Zhan LB, Liu T, Cheng MC, Liu XY and Xiao HB. Inhibitory effect of Ecliptae herba extract and its component wedelolactone on pre-osteoclastic proliferation and differentiation. J Ethnopharmacol 2014; 157: 206-211.","journal-title":"J Ethnopharmacol"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1016\/j.tips.2021.11.004","article-title":"Network pharmacology: curing causal mechanisms instead of treating symptoms","volume":"43","author":"Nogales","year":"2022","unstructured":"Nogales C, Mamdouh ZM, List M, Kiel C, Casas AI and Schmidt HHHW. Network pharmacology: curing causal mechanisms instead of treating symptoms. Trends Pharmacol Sci 2022; 43: 136-150.","journal-title":"Trends Pharmacol Sci"},{"key":"ref18","doi-asserted-by":"publisher","first-page":"110","DOI":"10.3724\/SP.J.1009.2013.00110","article-title":"Traditional Chinese medicine network pharmacology: theory, methodology and application","volume":"11","author":"Li","year":"2013","unstructured":"Li S and Zhang B. Traditional Chinese medicine network pharmacology: theory, methodology and application. Chin J Nat Med 2013; 11: 110-120.","journal-title":"Chin J Nat Med"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"501","DOI":"10.2174\/1389450119666181022153016","article-title":"Molecular docking: challenges, advances and its use in drug discovery perspective","volume":"20","author":"Saikia","year":"2019","unstructured":"Saikia S and Bordoloi M. Molecular docking: challenges, advances and its use in drug discovery perspective. Curr Drug Targets 2019; 20: 501-521.","journal-title":"Curr Drug Targets"},{"key":"ref20","doi-asserted-by":"publisher","first-page":"6311","DOI":"10.3390\/ijms20246311","article-title":"Acupuncture reduces apoptosis of granulosa cells in rats with premature ovarian failure via restoring the PI3K\/Akt signaling pathway","volume":"20","author":"Wang","year":"2019","unstructured":"Wang S, Lin S, Zhu M, Li C, Chen S, Pu L, Lin J, Cao L and Zhang Y. Acupuncture reduces apoptosis of granulosa cells in rats with premature ovarian failure via restoring the PI3K\/Akt signaling pathway. Int J Mol Sci 2019; 20: 6311.","journal-title":"Int J Mol Sci"},{"key":"ref21","doi-asserted-by":"publisher","unstructured":"McLean AC, Valenzuela N, Fai S and Bennett SA. Performing vaginal lavage, crystal violet staining, and vaginal cytological evaluation for mouse estrous cycle staging identification. J Vis Exp 2012; e4389.","DOI":"10.3791\/4389"},{"key":"ref22","doi-asserted-by":"publisher","first-page":"90","DOI":"10.5114\/pm.2016.61191","article-title":"Hematologic parameters as the predictors for metabolic syndrome in perimenopausal and postmenopausal women living in urban area: a preliminary report","volume":"15","author":"Vichinsartvichai","year":"2016","unstructured":"Vichinsartvichai P and Sirirat S. Hematologic parameters as the predictors for metabolic syndrome in perimenopausal and postmenopausal women living in urban area: a preliminary report. Prz Menopauzalny 2016; 15: 90-5.","journal-title":"Prz Menopauzalny"},{"key":"ref23","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1002\/ijc.29427","article-title":"Prospective study of body size throughout the life-course and the incidence of endometrial cancer among premenopausal and postmenopausal women","volume":"137","author":"Dougan","year":"2015","unstructured":"Dougan MM, Hankinson SE, Vivo ID, Tworoger SS, Glynn RJ and Michels KB. Prospective study of body size throughout the life-course and the incidence of endometrial cancer among premenopausal and postmenopausal women. Int J Cancer 2015; 137: 625-637.","journal-title":"Int J Cancer"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1007\/s00109-021-02055-5","article-title":"Premature ovarian insufficiency: pathogenesis and therapeutic potential of mesenchymal stem cell","volume":"99","author":"Takahashi","year":"2021","unstructured":"Takahashi A, Yousif A, Hong L and Chefetz I. Premature ovarian insufficiency: pathogenesis and therapeutic potential of mesenchymal stem cell. J Mol Med (Berl) 2021; 99: 637-650.","journal-title":"J Mol Med (Berl)"},{"key":"ref25","doi-asserted-by":"publisher","first-page":"682","DOI":"10.1038\/nchembio.118","article-title":"Network pharmacology: the next paradigm in drug discovery","volume":"4","author":"Hopkins","year":"2008","unstructured":"Hopkins AL. Network pharmacology: the next paradigm in drug discovery. Nat Chem Biol 2008; 4: 682-690.","journal-title":"Nat Chem Biol"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/j.neulet.2015.03.003","article-title":"Pratensein ameliorates β-amyloid-induced cognitive impairment in rats via reducing oxidative damage and restoring synapse and BDNF levels","volume":"592","author":"Liang","year":"2015","unstructured":"Liang C, Tan S, Huang Q, Lin J, Lu Z and Lin X. Pratensein ameliorates β-amyloid-induced cognitive impairment in rats via reducing oxidative damage and restoring synapse and BDNF levels. Neurosci Lett 2015; 592: 48-53.","journal-title":"Neurosci Lett"},{"key":"ref27","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1007\/s12272-017-0909-y","article-title":"Methyl caffeate and some plant constituents inhibit age-related inflammation: effects on senescence-associated secretory phenotype (SASP) formation","volume":"40","author":"Lim","year":"2017","unstructured":"Lim H, Park BK, Shin SY, Kwon YS and Kim HP. Methyl caffeate and some plant constituents inhibit age-related inflammation: effects on senescence-associated secretory phenotype (SASP) formation. Arch Pharm Res 2017; 40: 524-535.","journal-title":"Arch Pharm Res"},{"key":"ref28","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1186\/s12906-022-03829-1","article-title":"Anxiolytic-like effect of Suanzaoren-Wuweizi herb-pair and evidence for the involvement of the monoaminergic system in mice based on network pharmacology","volume":"23","author":"Liu","year":"2023","unstructured":"Liu J, Shi JL, Guo JY, Chen Y, Ma XJ, Wang SN, Zheng ZQ, Lin MX and He S. Anxiolytic-like effect of Suanzaoren-Wuweizi herb-pair and evidence for the involvement of the monoaminergic system in mice based on network pharmacology. BMC Complement Med Ther 2023; 23: 7.","journal-title":"BMC Complement Med Ther"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"1053","DOI":"10.1038\/s41419-020-03253-8","article-title":"Peroxiredoxin 4 protects against ovarian ageing by ameliorating D-galactose-induced oxidative damage in mice","volume":"11","author":"Liang","year":"2020","unstructured":"Liang X, Yan Z, Ma W, Qian Y, Zou X, Cui Y, Liu J and Meng Y. Peroxiredoxin 4 protects against ovarian ageing by ameliorating D-galactose-induced oxidative damage in mice. Cell Death Dis 2020; 11: 1053.","journal-title":"Cell Death Dis"},{"key":"ref30","doi-asserted-by":"publisher","first-page":"913","DOI":"10.1016\/j.bcp.2004.12.005","article-title":"Mitochondrial-dependent, reactive oxygen species-independent apoptosis by myricetin: roles of protein kinase C, cytochrome c, and caspase cascade","volume":"69","author":"CH","year":"2005","unstructured":"Ko CH, Shen SC, Hsu CS and Chen YC. Mitochondrial-dependent, reactive oxygen species-independent apoptosis by myricetin: roles of protein kinase C, cytochrome c, and caspase cascade. Biochem Pharmacol 2005; 69: 913-927.","journal-title":"Biochem Pharmacol"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"2089586","DOI":"10.1155\/2019\/2089586","article-title":"Polysaccharides of fructus corni improve ovarian function in mice with aging-associated perimenopause symptoms","volume":"2019","author":"Wang","year":"2019","unstructured":"Wang Y, Wu JZ, Li Y and Qi X. Polysaccharides of fructus corni improve ovarian function in mice with aging-associated perimenopause symptoms. Evid Based Complement Alternat Med 2019; 2019: 2089586.","journal-title":"Evid Based Complement Alternat Med"},{"key":"ref32","unstructured":"Zhang S, Zhou HF, Liu YN, Liu B, Yuan YZ, Shan JJ and Ji JJ. Modified Dihuang decoction improves ovarian reserve in mice by regulating Bcl-2-related mitochondrial apoptosis pathway. Zhongguo Zhong Yao Za Zhi 2021; 46: 6493-6501."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"3700","DOI":"10.3390\/molecules24203700","article-title":"PI3K-AKT signaling activation and icariin: the potential effects on the perimenopausal depression-like rat model","volume":"24","author":"Cao","year":"2019","unstructured":"Cao LH, Qiao JY, Huang HY, Fang XY, Zhang R, Miao MS and Li XM. PI3K-AKT signaling activation and icariin: the potential effects on the perimenopausal depression-like rat model. Molecules 2019; 24: 3700.","journal-title":"Molecules"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"8210","DOI":"10.3390\/ijms24098210","article-title":"Red ginseng improves d-galactose-induced premature ovarian failure in mice based on network pharmacology","volume":"24","author":"Shang","year":"2023","unstructured":"Shang Z, Fan M, Zhang J, Wang Z, Jiang S and Li W. Red ginseng improves d-galactose-induced premature ovarian failure in mice based on network pharmacology. Int J Mol Sci 2023; 24: 8210.","journal-title":"Int J Mol Sci"},{"key":"ref35","doi-asserted-by":"publisher","unstructured":"Zhang F, Cao J, Zhang X, Wang J and Yi X. Efficacy of self-made Gengnian decoction on phosphatidylinositol 3-kinases\/protein kinase B\/mammalian target of rapamycin signaling pathway in perimenopausal rats. J Tradit Chin Med 2019; 39: 861-866.","DOI":"10.3892\/etm.2018.5805"},{"key":"ref36","doi-asserted-by":"publisher","first-page":"634","DOI":"10.1038\/s43587-021-00098-4","article-title":"Autophagy in healthy aging and disease","volume":"1","author":"Aman","year":"2021","unstructured":"Aman Y, Schmauck-Medina T, Hansen M, Morimoto RI, Simon AK, Bjedov I, Palikaras K, Simonsen A, Johansen T, Tavernarakis N, Rubinsztein DC, Partridge L, Kroemer G, Labbadia J and Fang EF. Autophagy in healthy aging and disease. Nat Aging 2021; 1: 634-650.","journal-title":"Nat Aging"},{"key":"ref37","doi-asserted-by":"publisher","unstructured":"Tabibzadeh S. Role of autophagy in aging: the good, the bad, and the ugly. Aging Cell 2023; 22: e13753.","DOI":"10.1111\/acel.13753"},{"key":"ref38","doi-asserted-by":"publisher","first-page":"2532","DOI":"10.1016\/j.fertnstert.2009.11.021","article-title":"The role of autophagy in follicular development and atresia in rat granulosa cells","volume":"93","author":"Choi","year":"2010","unstructured":"Choi JY, Jo MW, Lee EY, Yoon BK and Choi DS. The role of autophagy in follicular development and atresia in rat granulosa cells. Fertil Steril 2010; 93: 2532-2537.","journal-title":"Fertil Steril"},{"key":"ref39","doi-asserted-by":"publisher","first-page":"2706","DOI":"10.1016\/j.fertnstert.2009.02.038","article-title":"The variable expression of lectin-like oxidized low-density lipoprotein receptor (LOX-1) and signs of autophagy and apoptosis in freshly harvested human granulosa cells depend on gonadotropin dose, age, and body weight","volume":"93","author":"Vilser","year":"2010","unstructured":"Vilser C, Hueller H, Nowicki M, Hmeidan FA, Blumenauer V and Spanel-Borowski K. The variable expression of lectin-like oxidized low-density lipoprotein receptor (LOX-1) and signs of autophagy and apoptosis in freshly harvested human granulosa cells depend on gonadotropin dose, age, and body weight. Fertil Steril 2010; 93: 2706-2715.","journal-title":"Fertil Steril"},{"key":"ref40","unstructured":"Jiang Y, Wang H, Yu X and Ding Y. Lycium barbarum polysaccharides regulate AMPK\/Sirt autophagy pathway to delay D-gal-induced premature ovarian failure. Zhongguo Zhong Yao Za Zhi 2022; 47: 6175-6182."}],"container-title":["American Journal of Translational Research"],"original-title":[],"link":[{"URL":"https:\/\/e-century.us\/files\/ajtr\/16\/6\/ajtr0155669.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T01:51:31Z","timestamp":1766022691000},"score":1,"resource":{"primary":{"URL":"https:\/\/e-century.us\/files\/ajtr\/16\/6\/ajtr0155669.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"references-count":41,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.62347\/rqhy5963","relation":{},"ISSN":["1943-8141"],"issn-type":[{"value":"1943-8141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024]]}}}