{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T17:00:13Z","timestamp":1778778013370,"version":"3.51.4"},"reference-count":0,"publisher":"Tunisian Society of Medical Sciences","issue":"2","license":[{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tunis Med"],"abstract":"<jats:p>Introduction : A la naissance, le diagnostic biologique de la toxoplasmose cong\u00e9nitale (TC) est essentiellement s\u00e9rologique.\nObjectif\u00a0: Rapporter les r\u00e9sultats des techniques biologiques utilis\u00e9es dans le diagnostic n\u00e9onatal de la TC.\nM\u00e9thodes : Il s'agissait d'une \u00e9tude descriptive incluant les nouveau-n\u00e9s (NN) suspects de TC adress\u00e9s au laboratoire de Parasitologie-Mycologie de l'Institut Pasteur de Tunis durant 17 ans. Les NN dont les m\u00e8res ont pr\u00e9sent\u00e9 une infection toxoplasmique au cours de la grossesse et pour lesquelles, un diagnostic ant\u00e9natal \u00e9tait soit n\u00e9gatif soit non r\u00e9alis\u00e9, ont \u00e9t\u00e9 collig\u00e9s. La recherche des anticorps IgG et IgM a \u00e9t\u00e9 faite par les techniques ELISA et western blot.\nR\u00e9sultats : Parmi les 224 NN inclus, le premier pr\u00e9l\u00e8vement a \u00e9t\u00e9 effectu\u00e9 entre J1 et J3 de vie dans 58% des cas. Pour 181 NN de notre s\u00e9rie, la disparition des IgG a \u00e9t\u00e9 observ\u00e9e en moyenne \u00e0 l\u2019\u00e2ge de 4,8 mois \u00b1 1,6. Les IgM \u00e9taient positives au-del\u00e0 de J10 de vie dans 11 cas. Un western blot comparatif m\u00e8re-NN a \u00e9t\u00e9 r\u00e9alis\u00e9 pour 216 NN (96,4%). Le diagnostic de TC a \u00e9t\u00e9 retenu chez 43 NN (19,2%), soit devant un western blot positif dans 30 cas (69,8%), soit devant la pr\u00e9sence d\u2019IgM par ELISA au-del\u00e0 de J10 de vie dans 11 cas (25,6%), soit devant le non-infl\u00e9chissement des IgG au bout de 12 mois de suivi dans deux cas (4,6%).\nConclusion : Notre travail confirme l\u2019apport ind\u00e9niable du western blot comparatif dans le diagnostic de la TC \u00e0 la naissance.\n\u00a0<\/jats:p>","DOI":"10.62438\/tunismed.v103i2.5431","type":"journal-article","created":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T17:26:41Z","timestamp":1765906001000},"source":"Crossref","is-referenced-by-count":1,"title":["Place des m\u00e9thodes biologiques dans le diagnostic n\u00e9onatal de la toxoplasmose cong\u00e9nitale en Tunisie"],"prefix":"10.62438","volume":"103","author":[{"given":"Rym","family":"Ben-Abdallah","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ines","family":"Bouhaoula","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olfa","family":"Souissi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rania","family":"Maatoug","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karim","family":"Aoun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aida","family":"Bouratbine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"31112","published-online":{"date-parts":[[2025,2,1]]},"container-title":["La Tunisie M\u00e9dicale"],"original-title":[],"deposited":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T17:26:41Z","timestamp":1765906001000},"score":1,"resource":{"primary":{"URL":"https:\/\/latunisiemedicale.com\/index.php\/tunismed\/article\/view\/5431"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,2,1]]}},"URL":"https:\/\/doi.org\/10.62438\/tunismed.v103i2.5431","relation":{},"ISSN":["2724-7031","0041-4131"],"issn-type":[{"value":"2724-7031","type":"electronic"},{"value":"0041-4131","type":"print"}],"subject":[],"published":{"date-parts":[[2025,2,1]]}}}