{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T02:35:23Z","timestamp":1783823723364,"version":"3.55.0"},"reference-count":0,"publisher":"Editorial Office of Chinese Journal of Applied Physiology","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CJAP"],"published-print":{"date-parts":[[2025]]},"abstract":"<jats:p>Traditional Chinese medicine (TCM) bioactives display wide pharmacological effects, yet bulk transcriptomic studies mask their cell-specific actions. This study applied single-cell RNA sequencing to delineate immune, neural and hepatic responses to chemically characterized TCM constituents. Murine splenic, hippocampal and hepatic tissues were dissociated into single-cell suspensions, processed on a 10\u00d7 Genomics platform, sequenced at high depth and analyzed with Seurat for clustering, marker annotation, differential expression and pathway enrichment; ligand\u2013receptor interactions were inferred by CellChat, with RT-qPCR and immunofluorescence validation. From over 42,000 high-quality cells, we resolved 15 immune, 12 neural and 10 hepatic subtypes. TCM exposure induced NF-\u03baB modulators in macrophages, neurotrophic gene expression in astrocytes and xenobiotic-metabolism programs in hepatocytes, while pathway analysis highlighted coordinated immune\u2013hepatic detoxification and neuroprotective signaling. Ligand\u2013receptor mapping revealed strengthened IL-10\u2013STAT3 and hepatocyte\u2013Kupffer cross-talk. These findings demonstrate that single-cell transcriptomics exposes previously hidden, cell-type-specific pharmacodynamics of TCM bioactives, laying a mechanistic foundation for precision-oriented herbal pharmacology and rational drug development.<\/jats:p>","DOI":"10.62958\/j.cjap.2025.028","type":"journal-article","created":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T00:54:10Z","timestamp":1762736050000},"page":"e20250028","source":"Crossref","is-referenced-by-count":3,"title":["Single Cell Transcriptomics of Traditional Chinese Medicine Bioactives: Mapping Immune, Neural and Hepatic Responses"],"prefix":"10.62958","author":[{"name":"School of Pharmacy, Rai University, Ahmedabad, Gujarat-382260 India","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shubham","family":"Singh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakshi","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"United Institute of Technology, Prayagraj, Uttar Pradesh, India","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayli","family":"Saw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"School of Pharmacy, Rai University, Ahmedabad, Gujarat-382260 India","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjesh","family":"Rathi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"School of Pharmacy, Rai University, Ahmedabad, Gujarat-382260 India","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhawna","family":"Sharma3","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"KM University, School of Pharmacy, Mathura, Uttar Pradesh, India","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"49313","published-online":{"date-parts":[[2025]]},"container-title":["Chinese Journal of Applied Physiology"],"original-title":[],"deposited":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T00:54:10Z","timestamp":1762736050000},"score":1,"resource":{"primary":{"URL":"http:\/\/47.94.85.12:8889\/article\/1987684400746426368"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":0,"URL":"https:\/\/doi.org\/10.62958\/j.cjap.2025.028","relation":{},"ISSN":["1000-6834"],"issn-type":[{"value":"1000-6834","type":"print"}],"subject":[],"published":{"date-parts":[[2025]]}}}