{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:09:11Z","timestamp":1785424151062,"version":"3.56.0"},"posted":{"date-parts":[[2025,12,2]]},"group-title":"Developmental Biology","reference-count":56,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nd\/4.0\/"}],"funder":[{"award":["227294\/Z\/23\/Z"],"award-info":[{"award-number":["227294\/Z\/23\/Z"]}],"id":[{"id":"https:\/\/ror.org\/029chgv08","id-type":"ROR","asserted-by":"publisher"}]},{"name":"NIH NINDS","award":["NINDS R01NS092096"],"award-info":[{"award-number":["NINDS R01NS092096"]}]},{"name":"NIH NINDS","award":["K99\/R00 NS112605-01"],"award-info":[{"award-number":["K99\/R00 NS112605-01"]}]},{"name":"NIH NIMH","award":["R37MH085726"],"award-info":[{"award-number":["R37MH085726"]}]},{"name":"National Cancer Institute Cancer Center Support Grant","award":["P30 CA008748-48"],"award-info":[{"award-number":["P30 CA008748-48"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,12,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  There is a crucial need for strategies that stimulate repair in the adult brain. The neonatal mouse cerebellum can regenerate via the adaptive reprogramming of nestin (Nes)-expressing progenitors (NEPs). However, analysis of\n                  <jats:italic>Nes<\/jats:italic>\n                  + cells of the adult cerebellum is limited. Using reporter lines and genetic inducible fate mapping, we show that adult\n                  <jats:italic>Nes<\/jats:italic>\n                  + cells are mainly Bergmann glia (\n                  <jats:italic>Nes<\/jats:italic>\n                  + Bg) that have\n                  <jats:italic>in vitro<\/jats:italic>\n                  sphere-forming ability. Following injury,\n                  <jats:italic>Nes<\/jats:italic>\n                  + Bg increase in number due to upregulation of\n                  <jats:italic>Nes<\/jats:italic>\n                  in\n                  <jats:italic>Hopx<\/jats:italic>\n                  -expressing Bg, but the cells exhibit limited regeneration. ATAC-seq of\n                  <jats:italic>Nes<\/jats:italic>\n                  + Bg reveals that silencing of developmental genes compared to neonatal NEPs contributes to the impaired regeneration. Activating sonic hedgehog signalling augments the number of\n                  <jats:italic>Nes<\/jats:italic>\n                  + Bg after injury but not neurogenesis, showing additional cues are required. Our results demonstrate an age-dependent decline in the regenerative potential of NEPs and highlight\n                  <jats:italic>Nes+<\/jats:italic>\n                  Bg as potential injury-responsive cells that could facilitate regeneration.\n                <\/jats:p>","DOI":"10.64898\/2025.12.01.691510","type":"posted-content","created":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T12:45:11Z","timestamp":1765197911000},"source":"Crossref","is-referenced-by-count":0,"title":["The regenerative potential of adult\n                  <i>Nestin+<\/i>\n                  cerebellar astroglia is limited compared to in neonates"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4371-855X","authenticated-orcid":false,"given":"N. Sumru","family":"Bayin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel N.","family":"Stephen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Koche","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7090-9605","authenticated-orcid":false,"given":"Alexandra 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