{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T07:53:25Z","timestamp":1767858805618,"version":"3.49.0"},"posted":{"date-parts":[[2025,12,2]]},"group-title":"Biochemistry","reference-count":81,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"U.S. National Science Foundation","doi-asserted-by":"crossref","award":["IIP-1143342"],"award-info":[{"award-number":["IIP-1143342"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000001","name":"U.S. National Science Foundation","doi-asserted-by":"crossref","award":["IIP-1330140"],"award-info":[{"award-number":["IIP-1330140"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Cancer Institute","award":["1R41CA203457-01A1"],"award-info":[{"award-number":["1R41CA203457-01A1"]}]},{"name":"National Cancer Institute","award":["1R44CA233128-01"],"award-info":[{"award-number":["1R44CA233128-01"]}]},{"name":"National Cancer Institute","award":["4R44CA233128-02"],"award-info":[{"award-number":["4R44CA233128-02"]}]},{"name":"National Cancer Institute","award":["4R44CA233128-03"],"award-info":[{"award-number":["4R44CA233128-03"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,12,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>Despite progress made in the development of anticancer therapeutics, traditional small-molecule chemotherapeutics often struggle to overcome toxicity, efficacy, and off-target effects. These specific issues can be overcome by either encapsulating the drug or by targeting it directly to the tumor cell. Here, we describe a novel targeted nanoparticle (referred to as a nano-antibody-drug conjugate Targeted Nanosphere or nADC\/TNS), based cancer therapeutic platform that can improve the efficacy of a broad range of existing therapeutics. Targeting is antibody-directed, as with antibody-drug conjugates (ADCs). Still, the payload per antibody is vastly greater by orders of magnitude (a thousand for nADC\/TNS versus two to eight for ADCs). The nADC\/TNS consists of an approximately 80 nm drug-filled nanoparticle composed of phospholipids, cholesterol, and UV cross-linkable diacetylene lipids. We describe the preparation, characterization, and evaluation of nADC\/TNS as a novel, versatile, and effective treatment modality for cancer and potentially other diseases. This report focuses on data with nADC\/TNS variants NV101 (anti-CD99 targeted, doxorubicin-filled), NV102 (anti-CD19 targeted, doxorubicin-filled), and NV103 (anti-CD99 targeted, irinotecan-filled). We investigated NV101 and NV103 in a mouse model with implanted and metastatic Ewing tumors (ES). NV101 demonstrated significant tumor burden reduction while NV103 induced complete ablation of ES tumors. NV102 demonstrated complete ablation of chemotherapy-resistant relapsed adult lymphocytic leukemia (ALL). These results document the potential superior efficacy of antibody-targeted nanoparticles containing a variety of small-molecule payloads, compared to their free molecule equivalents.<\/jats:p>","DOI":"10.64898\/2025.12.01.691687","type":"posted-content","created":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T12:47:45Z","timestamp":1765198065000},"source":"Crossref","is-referenced-by-count":1,"title":["Targeted Nanoparticles: an Innovative Modality in the Treatment of Cancer"],"prefix":"10.64898","author":[{"given":"Hyung-Gyoo","family":"Kang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryon","family":"Upton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan 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