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Despite the myriad of biotechnology applications that arose from characterization of the\n                  <jats:italic>lac<\/jats:italic>\n                  system, the explanation for the importance of a functional\n                  <jats:italic>lac<\/jats:italic>\n                  operon in normal bladder colonization by uropathogenic\n                  <jats:italic>E. coli<\/jats:italic>\n                  remains unknown given that the prototypical substrate, lactose, is not normally known to be readily available within the urinary tract. Here, we identified a unique uropathogenic clinical isolate (5.3r) that has a two codon deletion in the LacY permease, leading to impaired \u03b2-galactoside metabolism and attenuation of development of critical intracellular bacterial communities (IBCs) in which UPEC replicates to high numbers, and then disseminates during urinary tract infection. Further, we show that expression of a functional\n                  <jats:italic>lacY<\/jats:italic>\n                  permease gene is sufficient to rescue defects in IBC size and number in 5.3r. In addition, we demonstrate that UPEC are able to utilize the disaccharide galactose \u03b2-1,4 N-acetylglucosamine (LacNAc) \u2013 which appears as the terminal glycan in a subset of the glycoproteins that decorate the apical surface of bladder epithelial cells \u2013 as a sole carbon source in a\n                  <jats:italic>lac<\/jats:italic>\n                  system dependent manner. These data suggest that the\n                  <jats:italic>lac<\/jats:italic>\n                  operon is important to the growth and development of intracellular\n                  <jats:italic>E. coli<\/jats:italic>\n                  through metabolism of host bladder cell glycans.\n                <\/jats:p>","DOI":"10.64898\/2025.12.03.691977","type":"posted-content","created":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T12:43:26Z","timestamp":1765197806000},"source":"Crossref","is-referenced-by-count":0,"title":["The\n                  <i>lac<\/i>\n                  operon in uropathogenic\n                  <i>Escherichia coli<\/i>\n                  enhances intracellular growth by enabling host glycan utilization"],"prefix":"10.64898","author":[{"given":"Jesus Bazan","family":"Villicana","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Devina","family":"Puri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taylor M.","family":"Nye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca L.","family":"Hurto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennie","family":"Hazen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathaniel C.","family":"Gualberto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward D. 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