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Here, we identify a role for functional amyloid fibrils in seminal fluid that bind and traffic RNA to mammalian sperm and, upon fertilization, the zygote. We show that highly cationic peptides in seminal fluid phase separate with RNA to form amyloid fibrils. Cryo-electron tomography reveals that these amyloid fibrils are internalized by sperm. Furthermore, biophysical and\n                  <jats:italic>in vitro<\/jats:italic>\n                  fertilization experiments show that amyloid-bound RNA reaches the zygote where molecular chaperones control release of RNA for translation. Our work uncovers a mechanism for RNA trafficking from seminal fluid to the zygote, establishing amyloid fibrils as potential tools for RNA delivery during fertilization.\n                <\/jats:p>","DOI":"10.64898\/2025.12.10.693493","type":"posted-content","created":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T05:25:17Z","timestamp":1765517117000},"source":"Crossref","is-referenced-by-count":0,"title":["Amyloid-mediated RNA uptake by sperm for embryonic delivery"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3981-9702","authenticated-orcid":false,"given":"William S.","family":"Mallen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nataliya","family":"Zabello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan","family":"Brenner-Morton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9073-6641","authenticated-orcid":false,"given":"Simone","family":"Sidoli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0025-8549","authenticated-orcid":false,"given":"Isabella D.","family":"Pirozzolo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng-Xia","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8585-175X","authenticated-orcid":false,"given":"Joseph","family":"Sall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8637-3423","authenticated-orcid":false,"given":"Jason","family":"Yin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priyanka","family":"Mathews","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolyn L.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3527-7961","authenticated-orcid":false,"given":"Anthony W.P.","family":"Fitzpatrick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025121310201748000_2025.12.10.693493v1.1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad6780"},{"key":"2025121310201748000_2025.12.10.693493v1.2","doi-asserted-by":"crossref","first-page":"31794","DOI":"10.1038\/srep31794","article-title":"Characterisation of mouse epididymosomes reveals a complex profile of microRNAs and a potential mechanism for modification of the sperm epigenome","volume":"6","year":"2016","journal-title":"Scientific Reports"},{"key":"2025121310201748000_2025.12.10.693493v1.3","doi-asserted-by":"crossref","first-page":"5889","DOI":"10.3390\/ijms24065889","article-title":"Sperm RNA Payload: Implications for Intergenerational Epigenetic Inheritance","volume":"24","year":"2023","journal-title":"International Journal of Molecular Sciences"},{"key":"2025121310201748000_2025.12.10.693493v1.4","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad7977"},{"key":"2025121310201748000_2025.12.10.693493v1.5","doi-asserted-by":"publisher","DOI":"10.1038\/nrg.2016.106"},{"key":"2025121310201748000_2025.12.10.693493v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.06.023"},{"key":"2025121310201748000_2025.12.10.693493v1.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.06.024"},{"key":"2025121310201748000_2025.12.10.693493v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.10.014"},{"key":"2025121310201748000_2025.12.10.693493v1.9","doi-asserted-by":"crossref","unstructured":"N. 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