{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T12:03:52Z","timestamp":1766232232352,"version":"3.48.0"},"posted":{"date-parts":[[2025,12,17]]},"group-title":"Cell Biology","reference-count":10,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"DOI":"10.13039\/100011661","name":"PNNL","doi-asserted-by":"crossref","award":["DE-AC05-76RL01830"],"award-info":[{"award-number":["DE-AC05-76RL01830"]}],"id":[{"id":"10.13039\/100011661","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Blood Cancer United","award":["Scholar Award"],"award-info":[{"award-number":["Scholar Award"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01DK118072"],"award-info":[{"award-number":["R01DK118072"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01AG069010"],"award-info":[{"award-number":["R01AG069010"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["U01AG077925"],"award-info":[{"award-number":["U01AG077925"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["P30CA034196"],"award-info":[{"award-number":["P30CA034196"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,12,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Total-body irradiation (TBI) is routinely used for myeloablation prior to mouse hematopoietic cell transplant. Widespread transition from\n                  <jats:sup>137<\/jats:sup>\n                  Cs\n                  <jats:italic>\u03b3<\/jats:italic>\n                  -irradiators to X-ray systems has raised questions about whether these modalities yield equivalent biological outcomes. Although prior studies compared\n                  <jats:italic>\u03b3<\/jats:italic>\n                  and X-ray irradiation in healthy syngeneic transplants, their performance in reciprocal congenic models and in primary acute myeloid leukemia (AML) transplant remains unclear. Here, we systematically evaluated\n                  <jats:italic>\u03b3<\/jats:italic>\n                  and X-ray irradiation across dose and dose-rate conditions, and tested dose equivalents in CD45.1\/CD45.2 reciprocal transplants and in AML transplant models. While each modality exhibited distinct early effects, both ultimately supported comparable long-term donor chimerism in congenic transplants and equivalent AML engraftment, leukemic burden, and disease progression. These findings indicate that, with proper dose calibration, X-ray irradiation is a functionally effective alternative to\n                  <jats:italic>\u03b3<\/jats:italic>\n                  -irradiation for normal and malignant transplant studies.\n                <\/jats:p>","DOI":"10.64898\/2025.12.15.694472","type":"posted-content","created":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T06:45:11Z","timestamp":1766040311000},"source":"Crossref","is-referenced-by-count":0,"title":["Functional Equivalence of Gamma- and X-Ray Irradiation for Long-Term Hematopoietic and AML Transplant Outcomes in Mice"],"prefix":"10.64898","author":[{"given":"Shawn","family":"David","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Griffin J.","family":"Nye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewelina","family":"Bolcun-Filas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer J.","family":"Trowbridge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kira A.","family":"Young","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025122004000741000_2025.12.15.694472v1.1","first-page":"165","article-title":"Comparison of Cesium-137 and X-ray Irradiators by Using Bone Marrow Transplant Reconstitution in C57BL\/6J Mice","volume":"65","year":"2015","journal-title":"Comp Med"},{"key":"2025122004000741000_2025.12.15.694472v1.2","first-page":"1559325820916572","article-title":"A Comparison of Cs-137 gamma Rays and 320-kV X-Rays in a Mouse Bone Marrow Transplantation Model","volume":"18","year":"2020","journal-title":"Dose Response"},{"key":"2025122004000741000_2025.12.15.694472v1.3","doi-asserted-by":"crossref","first-page":"e0247501","DOI":"10.1371\/journal.pone.0247501","article-title":"Comparison of gamma and x-ray irradiation for myeloablation and establishment of normal and autoimmune syngeneic bone marrow chimeras","volume":"16","year":"2021","journal-title":"PLoS One"},{"key":"2025122004000741000_2025.12.15.694472v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-018-0368-6"},{"key":"2025122004000741000_2025.12.15.694472v1.5","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1703766"},{"key":"2025122004000741000_2025.12.15.694472v1.6","doi-asserted-by":"crossref","unstructured":"Bae MJ , Kang MK , Kye YU , et al. Differential Effects of Low and High Radiation Dose Rates on Mouse Spermatogenesis. Int J Mol Sci. 2021;22.","DOI":"10.3390\/ijms222312834"},{"key":"2025122004000741000_2025.12.15.694472v1.7","doi-asserted-by":"crossref","first-page":"e73745","DOI":"10.1371\/journal.pone.0073745","article-title":"Effect of radiation dose-rate on hematopoietic cell engraftment in adult zebrafish","volume":"8","year":"2013","journal-title":"PLoS One"},{"key":"2025122004000741000_2025.12.15.694472v1.8","doi-asserted-by":"publisher","DOI":"10.1667\/RR3197"},{"key":"2025122004000741000_2025.12.15.694472v1.9","first-page":"14 42 11","article-title":"Murine Retrovirally-Transduced Bone Marrow Engraftment Models of MLL-Fusion-Driven Acute Myelogenous Leukemias (AML)","volume":"78","year":"2017","journal-title":"Curr Protoc Pharmacol"},{"key":"2025122004000741000_2025.12.15.694472v1.10","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2018022400"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2025.12.15.694472","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T12:00:13Z","timestamp":1766232013000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2025.12.15.694472"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,17]]},"references-count":10,"URL":"https:\/\/doi.org\/10.64898\/2025.12.15.694472","relation":{},"subject":[],"published":{"date-parts":[[2025,12,17]]},"subtype":"preprint"}}