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Among these secondary colonizers,\n                  <jats:italic>Fusobacterium<\/jats:italic>\n                  species have documented associations with several gastrointestinal cancers. We found that both\n                  <jats:italic>F. animalis<\/jats:italic>\n                  and\n                  <jats:italic>F. nucleatum<\/jats:italic>\n                  invade cultured human gastric adenocarcinoma cells, but\n                  <jats:italic>F. animalis<\/jats:italic>\n                  exhibited higher adherence and invasion, and hypoxic conditions promoted higher bacterial survival. Both adherence and invasion were inhibited by exogenous GalNAc, a glycan commonly observed in membrane glycoproteins of adenocarcinoma cells, and a target of the fusobacterial adhesin Fap2. Using a mouse model of gastric metaplasia, we found that\n                  <jats:italic>F. animalis<\/jats:italic>\n                  colonized gastric tissue only after metaplasia onset, growing in multispecies biofilms in the mucus layer, while\n                  <jats:italic>F. nucleatum<\/jats:italic>\n                  colonized neither healthy nor metaplastic gastric tissue. Metaplasia led to upregulation of Gal-GalNAc in the stomach, and reduced gastric acidity allowed higher\n                  <jats:italic>F. animalis<\/jats:italic>\n                  loads in this model. By contrast, inflammation and the presence of\n                  <jats:italic>H. pylori<\/jats:italic>\n                  did not significantly influence stomach colonization by\n                  <jats:italic>F. animalis<\/jats:italic>\n                  . Overall, our data support a model in which\n                  <jats:italic>H. pylori<\/jats:italic>\n                  -induced metaplasia makes the stomach susceptible to secondary infection by another cancer-associated microbe,\n                  <jats:italic>F. animalis<\/jats:italic>\n                  .\n                <\/jats:p>","DOI":"10.64898\/2025.12.16.694801","type":"posted-content","created":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T15:55:13Z","timestamp":1766073313000},"source":"Crossref","is-referenced-by-count":1,"title":["Metaplasia Enables Stomach Colonization by\n                  <i>Fusobacterium animalis<\/i>"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4971-8999","authenticated-orcid":false,"given":"C.","family":"G\u00f3mez-Garz\u00f3n","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Q.","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.P.","family":"O\u2019Brien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2762-1424","authenticated-orcid":false,"given":"N.R.","family":"Salama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025122214203061000_2025.12.16.694801v2.1","doi-asserted-by":"publisher","DOI":"10.3322\/CAAC.21834"},{"key":"2025122214203061000_2025.12.16.694801v2.2","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(19)30488-7"},{"key":"2025122214203061000_2025.12.16.694801v2.3","first-page":"3554","article-title":"A 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