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The microdevice integrates deformable elastomeric channels with magnetic bead stacking to immobilize and concentrate AgNCs within confined nanoscopic junctions, thereby generating densely packed plasmonic hot-spot architectures that markedly enhance Surface-Enhanced Raman Scattering (SERS) efficiency. Time-resolved measurements revealed substantial increases in Raman intensity for both 4-mercaptobenzoic acid (4-MBA) and 4-aminothiophenol (4-ATP), outperforming corresponding off-chip assays and maintaining stable enhancement factors over a four-hour operational window. Furthermore, the platform successfully demonstrated multiplexed sensing capability: mixed-analyte experiments containing 4-MBA, 4-ATP, and cysteamine produced clearly distinguishable spectral fingerprints, even under serial dilution and dynamically pulsed sample delivery. Collectively, these results establish the bead-stacked nano-sieve as a versatile, label-free, and scalable diagnostic architecture capable of achieving high sensitivity and spectral fidelity under continuous-flow conditions. Our technology holds significant promise for real-time, non-invasive molecular surveillance, point-of-care diagnostics, and early disease monitoring in resource-limited or field-deployable settings.<\/jats:p>","DOI":"10.64898\/2025.12.17.695009","type":"posted-content","created":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T04:50:48Z","timestamp":1766206248000},"source":"Crossref","is-referenced-by-count":0,"title":["Nano-Sieve\u2013Enabled On-Chip Concentration and Multiplexed Detection of Raman-Dye\u2013Labeled 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