{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T06:00:33Z","timestamp":1782280833393,"version":"3.54.5"},"posted":{"date-parts":[[2025,12,29]]},"group-title":"Physiology","reference-count":101,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T00:00:00Z","timestamp":1766966400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"U.S. Department of Agriculture National Institute of Food and Agriculture [Research Capacity Fund","award":["7004451"],"award-info":[{"award-number":["7004451"]}]},{"name":"National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health","award":["R01DK132819"],"award-info":[{"award-number":["R01DK132819"]}]},{"name":"Laboratory start-up funding provided by the Purdue University College of Agriculture","award":["NA"],"award-info":[{"award-number":["NA"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,12,29]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Excessive oxidative stress drives lipid peroxidation and contributes to skeletal muscle atrophy in a range of musculoskeletal diseases. Polyunsaturated fatty acids (PUFAs) are essential components of muscle cell membrane phospholipids and are especially susceptible to peroxidation due to the presence of double bonds. Currently, therapeutic options targeting lipid peroxidation to prevent muscle wasting are limited. Substituting the hydrogen atom at the bis-allylic position with deuterium could conceivably limit lipid peroxidation while retaining enzymatic PUFA metabolism. Here we investigated the potential role of deuterated PUFAs (D-PUFAs) in protecting against muscle cell dysfunction under conditions of elevated oxidative stress. Both native (H-) and deuterated (D-) forms of long chain PUFAs including arachidonic acid (ARA), eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA) stimulated\n                  <jats:italic>in vitro<\/jats:italic>\n                  muscle cell growth and development in the absence of oxidative stress. D-ARA, D-EPA, D-DPA, and D-DHA each protected cultured myotubes against the deleterious effects of direct exposure to reactive oxygen species (ROS) by limiting lipid peroxidation. In contrast, H-ARA, H-EPA, H-DPA, and H-DHA each increased sensitivity to ROS-induced lipid peroxidation and exacerbated oxidative stress-induced muscle cell dysfunction. Deuterated short chain linoleic acid (D-LA), alpha linolenic acid (D-ALA), as well as D-ARA and D-EPA (but not D-DPA or D-DHA) also protected against the deleterious effects of ferroptosis inducer erastin on myogenic differentiation. Finally, D-PUFAs modulated local expression of endogenous antioxidant enzymes and muscle-specific protein ligases. Overall, our study suggests a promising role of D-PUFAs as novel therapeutics to protect against skeletal muscle dysfunction induced by oxidative stress.\n                <\/jats:p>","DOI":"10.64898\/2025.12.29.696779","type":"posted-content","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T23:55:13Z","timestamp":1767052513000},"source":"Crossref","is-referenced-by-count":0,"title":["Deuterated Polyunsaturated Fatty Acids Alleviate\n                  <i>In Vitro<\/i>\n                  Skeletal Muscle Dysfunction Induced by Oxidative Stress"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-9011-8711","authenticated-orcid":false,"given":"Xinyue","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Animal Sciences, College of Agriculture, Purdue University, West Lafayette, IN, USA"},{"name":"Interdepartmental Nutrition Program (INP), College of Health and Human Sciences, Purdue University, West Lafayette, IN, USA"},{"name":"Center for Aging and the Life Course (CALC), Purdue University, West Lafayette, IN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3472-0711","authenticated-orcid":false,"given":"Olga L.","family":"Sharko","sequence":"additional","affiliation":[{"name":"Institute of Physical Organic Chemistry, National Academy of Science, Minsk, Belarus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1775-8101","authenticated-orcid":false,"given":"Vadim V.","family":"Shmanai","sequence":"additional","affiliation":[{"name":"Institute of Physical Organic Chemistry, National Academy of Science, Minsk, Belarus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6154-1212","authenticated-orcid":false,"given":"Mikhail S.","family":"Shchepinov","sequence":"additional","affiliation":[{"name":"Center for Molecular and Cellular Biology, Moscow, Russia"},{"name":"Institut des Biomol\u00e9cules Max Mousseron, Universit\u00e9 de Montpellier, CNRS, ENSCM, Montpellier, France"},{"name":"Environmental and Occupational Health, School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5348-1464","authenticated-orcid":false,"given":"James F.","family":"Markworth","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, College of Agriculture, Purdue University, West Lafayette, IN, USA"},{"name":"Interdepartmental Nutrition Program (INP), College of Health and Human Sciences, Purdue University, West Lafayette, IN, USA"},{"name":"Center for Aging and the Life Course (CALC), Purdue University, West Lafayette, IN, USA"},{"name":"Purdue Institute of Inflammation, Immunology, and Infectious Disease (PI4D), Purdue University, West Lafayette, IN, USA"},{"name":"Indiana Center for Musculoskeletal Health (ICMH), Indiana University, Indianapolis, IN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025123112200475000_2025.12.29.696779v1.1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-014-9915-y"},{"key":"2025123112200475000_2025.12.29.696779v1.2","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/s13539-012-0074-6","article-title":"Molecular and cellular mechanisms of skeletal muscle atrophy: an update","volume":"3","year":"2012","journal-title":"J Cachexia Sarcopenia Muscle"},{"key":"2025123112200475000_2025.12.29.696779v1.3","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/2063179"},{"key":"2025123112200475000_2025.12.29.696779v1.4","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.redox.2015.09.005","article-title":"Reactive oxygen species and mitochondria: A nexus of cellular homeostasis","volume":"6","year":"2015","journal-title":"Redox Biol"},{"key":"2025123112200475000_2025.12.29.696779v1.5","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1007\/s10974-015-9438-9","article-title":"The role of oxidative stress in skeletal muscle injury and regeneration: focus on antioxidant enzymes","volume":"36","year":"2015","journal-title":"J Muscle Res Cell Motil"},{"key":"2025123112200475000_2025.12.29.696779v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2023.115664"},{"key":"2025123112200475000_2025.12.29.696779v1.7","doi-asserted-by":"crossref","first-page":"e13569","DOI":"10.1111\/acel.13569","article-title":"Scavenging mitochondrial hydrogen peroxide by peroxiredoxin 3 overexpression attenuates contractile dysfunction and muscle atrophy in a murine model of accelerated sarcopenia","volume":"21","year":"2022","journal-title":"Aging Cell"},{"key":"2025123112200475000_2025.12.29.696779v1.8","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-008-0216-7"},{"key":"2025123112200475000_2025.12.29.696779v1.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2014.02.023"},{"key":"2025123112200475000_2025.12.29.696779v1.10","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2017.7249"},{"key":"2025123112200475000_2025.12.29.696779v1.11","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-03613-y"},{"key":"2025123112200475000_2025.12.29.696779v1.12","doi-asserted-by":"crossref","first-page":"1226044","DOI":"10.3389\/fcell.2023.1226044","article-title":"ROS induced lipid peroxidation and their role in ferroptosis","volume":"11","year":"2023","journal-title":"Front Cell Dev Biol"},{"key":"2025123112200475000_2025.12.29.696779v1.13","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/360438"},{"key":"2025123112200475000_2025.12.29.696779v1.14","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-020-0565-1"},{"key":"2025123112200475000_2025.12.29.696779v1.15","doi-asserted-by":"publisher","DOI":"10.1021\/bi00181a003"},{"key":"2025123112200475000_2025.12.29.696779v1.16","doi-asserted-by":"publisher","DOI":"10.1016\/S0026-0495(98)90202-5"},{"key":"2025123112200475000_2025.12.29.696779v1.17","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R700051200"},{"key":"2025123112200475000_2025.12.29.696779v1.18","doi-asserted-by":"publisher","DOI":"10.1007\/BF02536034"},{"key":"2025123112200475000_2025.12.29.696779v1.19","first-page":"1406","article-title":"[Generation of the superoxide radical and lipid peroxidation in skeletal muscles]","volume":"50","year":"1985","journal-title":"Biokhimiia"},{"key":"2025123112200475000_2025.12.29.696779v1.20","doi-asserted-by":"crossref","first-page":"129487","DOI":"10.1016\/j.bbagen.2019.129487","article-title":"Lipid peroxidation is involved in calcium dependent upregulation of mitochondrial metabolism in skeletal muscle","volume":"1864","year":"2020","journal-title":"Biochim Biophys Acta Gen Subj"},{"key":"2025123112200475000_2025.12.29.696779v1.21","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2008.046045"},{"key":"2025123112200475000_2025.12.29.696779v1.22","doi-asserted-by":"crossref","first-page":"9551","DOI":"10.1096\/fj.201802724RR","article-title":"Iron accumulation causes impaired myogenesis correlated with MAPK signaling pathway inhibition by oxidative stress","volume":"33","year":"2019","journal-title":"FASEB J"},{"key":"2025123112200475000_2025.12.29.696779v1.23","doi-asserted-by":"crossref","unstructured":"Eshima, H. , Shahtout, J. L. , Siripoksup, P. , Pearson, M. J. , Mahmassani, Z. S. , Ferrara, P. J. , Lyons, A. W. , Maschek, J. A. , Peterlin, A. D. , Verkerke, A. R. P. , Johnson, J. M. , Salcedo, A. , Petrocelli, J. J. , Miranda, E. R. , Anderson, E. J. , Boudina, S. , Ran, Q. , Cox, J. E. , Drummond, M. J. , and Funai, K . (2023) Lipid hydroperoxides promote sarcopenia through carbonyl stress. Elife 12","DOI":"10.7554\/eLife.85289"},{"key":"2025123112200475000_2025.12.29.696779v1.24","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12700"},{"key":"2025123112200475000_2025.12.29.696779v1.25","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.12.4914-4923.2005"},{"key":"2025123112200475000_2025.12.29.696779v1.26","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.87.4.289"},{"key":"2025123112200475000_2025.12.29.696779v1.27","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200421146"},{"key":"2025123112200475000_2025.12.29.696779v1.28","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201800311"},{"key":"2025123112200475000_2025.12.29.696779v1.29","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2011.4391"},{"key":"2025123112200475000_2025.12.29.696779v1.30","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.12.010"},{"key":"2025123112200475000_2025.12.29.696779v1.31","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.02523"},{"key":"2025123112200475000_2025.12.29.696779v1.32","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaw9872"},{"key":"2025123112200475000_2025.12.29.696779v1.33","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)35980-5"},{"key":"2025123112200475000_2025.12.29.696779v1.34","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgi222"},{"key":"2025123112200475000_2025.12.29.696779v1.35","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63335-4"},{"key":"2025123112200475000_2025.12.29.696779v1.36","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.53126"},{"key":"2025123112200475000_2025.12.29.696779v1.37","doi-asserted-by":"publisher","DOI":"10.1016\/J.CMET.2023.02.001"},{"key":"2025123112200475000_2025.12.29.696779v1.38","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.680090"},{"key":"2025123112200475000_2025.12.29.696779v1.39","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1016\/j.drudis.2020.03.014","article-title":"Site-specifically deuterated essential lipids as new drugs against neuronal, retinal and vascular degeneration","volume":"25","year":"2020","journal-title":"Drug Discov Today"},{"key":"2025123112200475000_2025.12.29.696779v1.40","doi-asserted-by":"crossref","unstructured":"Westheimer, F. H. (1961) THE MAGNITUDE OF THE PRIMARY KINETIC ISOTOPE EFFECT FOR COMPOUNDS OF HYDROGEN AND DEUTERIUM. Chem. Revs. Vol: 61","DOI":"10.1021\/cr60211a004"},{"key":"2025123112200475000_2025.12.29.696779v1.41","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2020.01.010"},{"key":"2025123112200475000_2025.12.29.696779v1.42","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2015.00091"},{"key":"2025123112200475000_2025.12.29.696779v1.43","doi-asserted-by":"publisher","DOI":"10.1038\/s41583-019-0132-6"},{"key":"2025123112200475000_2025.12.29.696779v1.44","doi-asserted-by":"crossref","first-page":"461","DOI":"10.3233\/JPD-130230","article-title":"The role of oxidative stress in Parkinson\u2019s disease","volume":"3","year":"2013","journal-title":"J Parkinsons Dis"},{"key":"2025123112200475000_2025.12.29.696779v1.45","doi-asserted-by":"crossref","first-page":"4083","DOI":"10.1111\/febs.14291","article-title":"Deuterium-reinforced polyunsaturated fatty acids improve cognition in a mouse model of sporadic Alzheimer\u2019s disease","volume":"284","year":"2017","journal-title":"FEBS J"},{"key":"2025123112200475000_2025.12.29.696779v1.46","doi-asserted-by":"crossref","first-page":"641","DOI":"10.3389\/fphys.2019.00641","article-title":"Deuterated Polyunsaturated Fatty Acids Reduce Oxidative Stress and Extend the Lifespan of","volume":"10","year":"2019","journal-title":"Front Physiol"},{"key":"2025123112200475000_2025.12.29.696779v1.47","doi-asserted-by":"crossref","first-page":"111778","DOI":"10.1016\/j.mad.2023.111778","article-title":"Deuterated polyunsaturated fatty acids provided protection against oxidative stress in ocular fibroblasts derived from glaucoma patients","volume":"211","year":"2023","journal-title":"Mech Ageing Dev"},{"key":"2025123112200475000_2025.12.29.696779v1.48","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2017.06.916"},{"key":"2025123112200475000_2025.12.29.696779v1.49","doi-asserted-by":"crossref","unstructured":"Li, H. , Zhang, O. , Hui, C. , Huang, Y. , Shao, H. , Song, M. , Gao, L. , Jin, S. , Ding, C. , and Xu, L . (2022) Deuterium-Reinforced Polyunsaturated Fatty Acids Prevent Diet-Induced Nonalcoholic Steatohepatitis by Reducing Oxidative Stress. Medicina (Kaunas ) 58","DOI":"10.3390\/medicina58060790"},{"key":"2025123112200475000_2025.12.29.696779v1.50","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00038.2012"},{"key":"2025123112200475000_2025.12.29.696779v1.51","doi-asserted-by":"crossref","unstructured":"Risha, M. A. , Siengdee, P. , Dannenberger, D. , Wimmers, K. , and Ponsuksili, S . (2021) PUFA Treatment Affects C2C12 Myocyte Differentiation, Myogenesis Related Genes and Energy Metabolism. Genes (Basel ) 12","DOI":"10.3390\/genes12020192"},{"key":"2025123112200475000_2025.12.29.696779v1.52","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.00563"},{"key":"2025123112200475000_2025.12.29.696779v1.53","doi-asserted-by":"crossref","unstructured":"Zhang, J. , Xu, X. , Liu, Y. , Zhang, L. , Odle, J. , Lin, X. , Zhu, H. , and Wang, X . (2019) EPA and DHA Inhibit Myogenesis and Downregulate the Expression of Muscle-related Genes in C2C12 Myoblasts. Genes (Basel ) 10","DOI":"10.3390\/genes10010064"},{"key":"2025123112200475000_2025.12.29.696779v1.54","doi-asserted-by":"crossref","first-page":"116936","DOI":"10.1016\/j.taap.2024.116936","article-title":"AhR signaling modulates Ferroptosis by regulating SLC7A11 expression","volume":"486","year":"2024","journal-title":"Toxicol Appl Pharmacol"},{"key":"2025123112200475000_2025.12.29.696779v1.55","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-018-0178-0"},{"key":"2025123112200475000_2025.12.29.696779v1.56","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.5162"},{"key":"2025123112200475000_2025.12.29.696779v1.57","doi-asserted-by":"crossref","first-page":"109971","DOI":"10.1016\/j.celrep.2021.109971","article-title":"Integrated genomic and proteomic analyses identify stimulus-dependent molecular changes associated with distinct modes of skeletal muscle atrophy","volume":"37","year":"2021","journal-title":"Cell Rep"},{"key":"2025123112200475000_2025.12.29.696779v1.58","doi-asserted-by":"crossref","unstructured":"Lian, D. , Chen, M. M. , Wu, H. , Deng, S. , and Hu, X . (2022) The Role of Oxidative Stress in Skeletal Muscle Myogenesis and Muscle Disease. Antioxidants (Basel ) 11","DOI":"10.3390\/antiox11040755"},{"key":"2025123112200475000_2025.12.29.696779v1.59","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.freeradbiomed.2025.02.015","article-title":"Deuterium-reinforced polyunsaturated fatty acids protect against muscle atrophy by STZ-induced diabetic mice","volume":"230","year":"2025","journal-title":"Free Radic Biol Med"},{"key":"2025123112200475000_2025.12.29.696779v1.60","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/j.jbiosc.2020.12.010","article-title":"Effect of hydrogen peroxide concentration on the maintenance and differentiation of cultured skeletal muscle cells","volume":"131","year":"2021","journal-title":"J Biosci Bioeng"},{"key":"2025123112200475000_2025.12.29.696779v1.61","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-024-07244-x"},{"key":"2025123112200475000_2025.12.29.696779v1.62","doi-asserted-by":"publisher","DOI":"10.1007\/BF02533996"},{"key":"2025123112200475000_2025.12.29.696779v1.63","doi-asserted-by":"crossref","first-page":"2099","DOI":"10.1111\/febs.14807","article-title":"Threshold protective effect of deuterated polyunsaturated fatty acids on peroxidation of lipid bilayers","volume":"286","year":"2019","journal-title":"FEBS J"},{"key":"2025123112200475000_2025.12.29.696779v1.64","first-page":"845379","article-title":"Morphofunctional and Biochemical Approaches for Studying Mitochondrial Changes during Myoblasts Differentiation","volume":"2011","year":"2011","journal-title":"J Aging Res"},{"key":"2025123112200475000_2025.12.29.696779v1.65","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118350"},{"key":"2025123112200475000_2025.12.29.696779v1.66","doi-asserted-by":"crossref","first-page":"122356","DOI":"10.1016\/j.lfs.2023.122356","article-title":"Important roles of linoleic acid and \u03b1-linolenic acid in regulating cognitive impairment and neuropsychiatric issues in metabolic-related dementia","volume":"337","year":"2024","journal-title":"Life Sci"},{"key":"2025123112200475000_2025.12.29.696779v1.67","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00346.2016"},{"key":"2025123112200475000_2025.12.29.696779v1.68","doi-asserted-by":"crossref","unstructured":"Case, L. P. , Daristotle, L. , Hayek, M. G. , and and Raasch, M. F . (2011) Fat Requirements. In Canine and Feline Nutrition ( Case, L. P. , Daristotle, L. , Hayek, M. G. , and Raasch, M. F. , eds) pp. 81\u201388, Mosby, Saint Louis","DOI":"10.1016\/B978-0-323-06619-8.10011-8"},{"key":"2025123112200475000_2025.12.29.696779v1.69","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1603244113"},{"key":"2025123112200475000_2025.12.29.696779v1.70","doi-asserted-by":"crossref","unstructured":"Shchepinov, M. S. , Roginsky, V. A. , Brenna, J. T. , Molinari, R. J. , To, R. , Tsui, H. , Clarke, C. F. , and B, M.-B. A. (2014) Deuterium Protection of Polyunsaturated Fatty Acids against Lipid Peroxidation : A Novel Approach to Mitigating Mitochondrial Neurological Diseases. In Omega-3 Fatty Acids in Brain and Neurological Health ( Watson, R. R. , and De, M. F. , eds) pp. 373\u2013383, Academic Press","DOI":"10.1016\/B978-0-12-410527-0.00031-4"},{"key":"2025123112200475000_2025.12.29.696779v1.71","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-04273-8"},{"key":"2025123112200475000_2025.12.29.696779v1.72","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.110267"},{"key":"2025123112200475000_2025.12.29.696779v1.73","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0134227"},{"key":"2025123112200475000_2025.12.29.696779v1.74","doi-asserted-by":"crossref","first-page":"1381417","DOI":"10.3389\/fcell.2024.1381417","article-title":"Mitochondrial stress response and myogenic differentiation","volume":"12","year":"2024","journal-title":"Front Cell Dev Biol"},{"key":"2025123112200475000_2025.12.29.696779v1.75","first-page":"84","article-title":"How erastin assassinates cells by ferroptosis revealed","volume":"14","year":"2023","journal-title":"Protein Cell"},{"key":"2025123112200475000_2025.12.29.696779v1.76","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-022-00642-w"},{"key":"2025123112200475000_2025.12.29.696779v1.77","doi-asserted-by":"crossref","first-page":"101254","DOI":"10.1016\/j.gendis.2024.101254","article-title":"Regulation of SLC7A11 as an unconventional checkpoint in tumorigenesis through ferroptosis","volume":"12","year":"2025","journal-title":"Genes Dis"},{"key":"2025123112200475000_2025.12.29.696779v1.78","doi-asserted-by":"publisher","DOI":"10.1038\/srep30033"},{"key":"2025123112200475000_2025.12.29.696779v1.79","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-020-0496-x"},{"key":"2025123112200475000_2025.12.29.696779v1.80","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-39401-9"},{"key":"2025123112200475000_2025.12.29.696779v1.81","doi-asserted-by":"crossref","first-page":"833642","DOI":"10.3389\/fcvm.2022.833642","article-title":"Identification of HMOX1 as a Critical Ferroptosis-Related Gene in Atherosclerosis","volume":"9","year":"2022","journal-title":"Front Cardiovasc Med"},{"key":"2025123112200475000_2025.12.29.696779v1.82","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-020-00441-1"},{"key":"2025123112200475000_2025.12.29.696779v1.83","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20120936"},{"key":"2025123112200475000_2025.12.29.696779v1.84","doi-asserted-by":"publisher","DOI":"10.1172\/JCI99032"},{"key":"2025123112200475000_2025.12.29.696779v1.85","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.13.13.1800"},{"key":"2025123112200475000_2025.12.29.696779v1.86","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2017.12.025"},{"key":"2025123112200475000_2025.12.29.696779v1.87","doi-asserted-by":"publisher","DOI":"10.1111\/febs.12338"},{"key":"2025123112200475000_2025.12.29.696779v1.88","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2007.11.011"},{"key":"2025123112200475000_2025.12.29.696779v1.89","doi-asserted-by":"publisher","DOI":"10.1186\/s40064-016-2197-2"},{"key":"2025123112200475000_2025.12.29.696779v1.90","doi-asserted-by":"crossref","first-page":"155639","DOI":"10.1016\/j.metabol.2023.155639","article-title":"Sarcopenic obesity: emerging mechanisms and therapeutic potential","volume":"146","year":"2023","journal-title":"Metabolism"},{"key":"2025123112200475000_2025.12.29.696779v1.91","doi-asserted-by":"crossref","first-page":"154766","DOI":"10.1016\/j.phymed.2023.154766","article-title":"Saikosaponin A and D attenuate skeletal muscle atrophy in chronic kidney disease by reducing oxidative stress through activation of PI3K\/AKT\/Nrf2 pathway","volume":"114","year":"2023","journal-title":"Phytomedicine"},{"key":"2025123112200475000_2025.12.29.696779v1.92","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2022.0149"},{"key":"2025123112200475000_2025.12.29.696779v1.93","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00418.2001"},{"key":"2025123112200475000_2025.12.29.696779v1.94","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.12.10.871"},{"key":"2025123112200475000_2025.12.29.696779v1.95","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2020.06.001"},{"key":"2025123112200475000_2025.12.29.696779v1.96","doi-asserted-by":"crossref","first-page":"2606928","DOI":"10.1155\/2022\/2606928","article-title":"Reactive Oxygen Species Bridge the Gap between Chronic Inflammation and Tumor Development","volume":"2022","year":"2022","journal-title":"Oxid Med Cell Longev"},{"key":"2025123112200475000_2025.12.29.696779v1.97","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2007.10.047"},{"key":"2025123112200475000_2025.12.29.696779v1.98","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00249.2004"},{"key":"2025123112200475000_2025.12.29.696779v1.99","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2004.08.009"},{"key":"2025123112200475000_2025.12.29.696779v1.100","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2007.11.001"},{"key":"2025123112200475000_2025.12.29.696779v1.101","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2014.03.017"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2025.12.29.696779","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T05:07:30Z","timestamp":1782277650000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2025.12.29.696779"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,29]]},"references-count":101,"URL":"https:\/\/doi.org\/10.64898\/2025.12.29.696779","relation":{"is-preprint-of":[{"id-type":"doi","id":"10.1016\/j.freeradbiomed.2026.02.037","asserted-by":"subject"}]},"subject":[],"published":{"date-parts":[[2025,12,29]]},"subtype":"preprint"}}