{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T06:03:24Z","timestamp":1782281004897,"version":"3.54.5"},"posted":{"date-parts":[[2026,1,2]]},"group-title":"Biochemistry","reference-count":86,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T00:00:00Z","timestamp":1767312000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,1,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                  is a major cause of invasive aspergillosis in immunocompromised patients, where current antifungal therapies are limited by toxicity, drug resistance, and lack of durable protection, and no vaccines are available. A mutant lacking the sterylglucosidase-encoding gene (\n                  <jats:italic>sglA<\/jats:italic>\n                  ) has emerged as a candidate that induces protective immune responses, but the structural basis for this phenotype remains unclear. Here, we use cellular solid-state NMR spectroscopy to compare the organization of the conidial cell wall in \u0394\n                  <jats:italic>sglA<\/jats:italic>\n                  and its wild-type counterpart. The \u0394\n                  <jats:italic>sglA<\/jats:italic>\n                  conidial cell wall displays extensive remodeling, including increased \u03b1-1,3-glucan content and structural polymorphism, strengthened interactions with \u03b2-glucans, reduced hydration, and restricted molecular motion, together consolidating a more rigid scaffold with limited \u03b2-glucan accessibility. These structural changes are associated with altered neutrophil responses and a shift in innate immune signaling. This work links cell-wall reorganization to altered immune recognition in this vaccine candidate, with implications for future immunotherapeutic strategies.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>TEASER<\/jats:title>\n                  <jats:p>Molecular-level Insights from a fungal vaccine candidate show how cell-wall remodeling could affect immune response.<\/jats:p>\n                <\/jats:sec>","DOI":"10.64898\/2025.12.31.697239","type":"posted-content","created":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T10:40:14Z","timestamp":1767350414000},"source":"Crossref","is-referenced-by-count":0,"title":["\u03b1-1,3-Glucan-Driven Remodeling of the Conidial Cell Wall in an\n                  <i>Aspergillus fumigatus<\/i>\n                  Vaccine Strain Alters Innate Immune Recognition"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-2685-1091","authenticated-orcid":false,"given":"Kalpana","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Chemistry, Michigan State University, East Lansing, MI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ankur","family":"Ankur","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Michigan State University, East Lansing, MI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jayasubba Reddy","family":"Yarava","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Michigan State University, East Lansing, MI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline Mota","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Stony Brook University, Stony Brook, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianluca","family":"Vascelli","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery, University of Perugia, Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessia","family":"Sulla","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery, University of Perugia, Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Zelante","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery, University of Perugia, Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8418-3120","authenticated-orcid":false,"given":"Maurizio","family":"Del Poeta","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Stony Brook University, Stony Brook, NY, USA"},{"name":"Division of Infectious Diseases, Stony Brook University, Stony Brook, NY, USA"},{"name":"Veterans Affairs Medical Center, Northport, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuo","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Michigan State University, East Lansing, MI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026010502500442000_2025.12.31.697239v1.1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3004404"},{"key":"2026010502500442000_2025.12.31.697239v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(23)00692-8"},{"key":"2026010502500442000_2025.12.31.697239v1.3","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-024-01062-w"},{"key":"2026010502500442000_2025.12.31.697239v1.4","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00055-08"},{"key":"2026010502500442000_2025.12.31.697239v1.5","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.2.310"},{"key":"2026010502500442000_2025.12.31.697239v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2011.07.006"},{"key":"2026010502500442000_2025.12.31.697239v1.7","doi-asserted-by":"crossref","unstructured":"Wishart, W. 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