{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T06:04:21Z","timestamp":1782281061539,"version":"3.54.5"},"posted":{"date-parts":[[2026,1,7]]},"group-title":"Genetics","reference-count":34,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T00:00:00Z","timestamp":1767744000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["1R35GM118127"],"award-info":[{"award-number":["1R35GM118127"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["5T32GM007092"],"award-info":[{"award-number":["5T32GM007092"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"crossref","award":["1F31AG074637"],"award-info":[{"award-number":["1F31AG074637"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,1,7]]},"abstract":"<jats:p>\n                  Failures in chromosome segregation during meiosis result in aneuploid gametes and are the leading cause of miscarriage. The position and number of crossovers, genetic exchanges between homologous chromosomes, are essential to their accurate disjunction in meiosis. Previous research analyzing nondisjunction of acro- and telocentric chromosomes in human and\n                  <jats:italic>Drosophila<\/jats:italic>\n                  has identified altered positioning and number of crossovers that differs between meiosis I and meiosis II nondisjunction. Limited data from positioning in chromosomes that segregated normally in metacentric trisomies has suggested discrepancies between the behavior of these chromosomes and the acro\/telocentrics in nondisjunction, which warrants further investigation. Here, we employ whole-genome sequencing to study spontaneous meiotic nondisjunction of the metacentric chromosome\n                  <jats:italic>2<\/jats:italic>\n                  in\n                  <jats:italic>Drosophila melanogaster<\/jats:italic>\n                  . Several patterns of recombination may differentially impact telo- versus metacentric chromosomes: lack of a crossover, distal crossovers, and proximal crossovers. We find that unlike meiotic nondisjunction of the\n                  <jats:italic>Drosophila X<\/jats:italic>\n                  chromosome, nondisjunction of chromosome\n                  <jats:italic>2<\/jats:italic>\n                  is not associated with dramatic changes in crossover landscape, but is associated with reduced recombination. Differences in the proportions of NDJ events with altered recombination patterns between chromosomes\n                  <jats:italic>X<\/jats:italic>\n                  and\n                  <jats:italic>2<\/jats:italic>\n                  suggest that abnormal crossover positions disparately affect chromosomes with different shapes. Taken together, these findings highlight that the underlying triggers of meiotic nondisjunction are chromosome-specific.\n                <\/jats:p>","DOI":"10.64898\/2026.01.06.697974","type":"posted-content","created":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T23:05:10Z","timestamp":1767827110000},"source":"Crossref","is-referenced-by-count":1,"title":["Chromosome-specific differences in the recombination landscape of spontaneous meiotic nondisjunction"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6607-131X","authenticated-orcid":false,"given":"Carolyn A.","family":"Turcotte","sequence":"first","affiliation":[{"name":"Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4424-677X","authenticated-orcid":false,"given":"Jeff","family":"Sekelsky","sequence":"additional","affiliation":[{"name":"Integrative Program for Biological and Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA"},{"name":"Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"issue":"5461","key":"2026010907350822000_2026.01.06.697974v1.1","doi-asserted-by":"crossref","first-page":"2185","DOI":"10.1126\/science.287.5461.2185","article-title":"The genome sequence of Drosophila melanogaster","volume":"287","year":"2000","journal-title":"Science (New York, N.Y"},{"key":"2026010907350822000_2026.01.06.697974v1.2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/71.2.255"},{"key":"2026010907350822000_2026.01.06.697974v1.3","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/7.4.661"},{"issue":"4","key":"2026010907350822000_2026.01.06.697974v1.4","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1002\/pd.1123","article-title":"A comparison of maternal age, sex ratio and associated major anomalies among fetal trisomy 18 cases with different cell division of error","volume":"25","year":"2005","journal-title":"Prenatal Diagnosis"},{"issue":"10","key":"2026010907350822000_2026.01.06.697974v1.5","doi-asserted-by":"crossref","first-page":"e1002905","DOI":"10.1371\/journal.pgen.1002905","article-title":"The many landscapes of recombination in Drosophila melanogaster","volume":"8","year":"2012","journal-title":"PLOS Genetics"},{"key":"2026010907350822000_2026.01.06.697974v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2018.07.004"},{"issue":"2","key":"2026010907350822000_2026.01.06.697974v1.7","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1508\/cytologia.3.169","article-title":"The origin and behavior of chiasmata, II. Stenobothrus parallelus","volume":"3","year":"1932","journal-title":"Cytologia"},{"issue":"3","key":"2026010907350822000_2026.01.06.697974v1.8","first-page":"669","article-title":"Trisomy 18: studies of the parent and cell division of origin and the effect of aberrant recombination on nondisjunction","volume":"56","year":"1995","journal-title":"American Journal of Human Genetics"},{"key":"2026010907350822000_2026.01.06.697974v1.9","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.119.302509"},{"issue":"4","key":"2026010907350822000_2026.01.06.697974v1.10","first-page":"867","article-title":"Recombination and maternal age-dependent nondisjunction: molecular studies of trisomy 16","volume":"57","year":"1995","journal-title":"American Journal of Human Genetics"},{"key":"2026010907350822000_2026.01.06.697974v1.11","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.28.3.159"},{"key":"2026010907350822000_2026.01.06.697974v1.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2020.11.010"},{"key":"2026010907350822000_2026.01.06.697974v1.13","doi-asserted-by":"publisher","DOI":"10.1038\/ng1296-406"},{"key":"2026010907350822000_2026.01.06.697974v1.14","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/5.supplement_1.1495"},{"key":"2026010907350822000_2026.01.06.697974v1.15","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/6.9.1391"},{"key":"2026010907350822000_2026.01.06.697974v1.16","doi-asserted-by":"publisher","DOI":"10.1038\/ng1296-400"},{"issue":"9","key":"2026010907350822000_2026.01.06.697974v1.17","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1038\/nmeth.3930","article-title":"Real-time selective sequencing using nanopore technology","volume":"13","year":"2016","journal-title":"Nature Methods"},{"key":"2026010907350822000_2026.01.06.697974v1.18","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/24.3.413"},{"key":"2026010907350822000_2026.01.06.697974v1.19","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddt433"},{"key":"2026010907350822000_2026.01.06.697974v1.20","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.115.186486"},{"issue":"2","key":"2026010907350822000_2026.01.06.697974v1.21","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1534\/g3.111.001396","article-title":"A whole-chromosome analysis of meiotic recombination in Drosophila melanogaster","volume":"2","year":"2012","journal-title":"G3"},{"key":"2026010907350822000_2026.01.06.697974v1.22","doi-asserted-by":"publisher","DOI":"10.1126\/science.32.812.120"},{"issue":"2","key":"2026010907350822000_2026.01.06.697974v1.23","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1093\/genetics\/98.2.257","article-title":"The entire compound autosomes of Drosophila melanogaster","volume":"98","year":"1981","journal-title":"Genetics"},{"key":"2026010907350822000_2026.01.06.697974v1.24","doi-asserted-by":"publisher","DOI":"10.1038\/ng1296-374"},{"key":"2026010907350822000_2026.01.06.697974v1.25","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyad211"},{"key":"2026010907350822000_2026.01.06.697974v1.26","doi-asserted-by":"publisher","DOI":"10.4161\/fly.4720"},{"issue":"3","key":"2026010907350822000_2026.01.06.697974v1.27","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1093\/genetics\/73.3.465","article-title":"A meiotic mutant affecting recombination in female Drosophila melanogaster","volume":"73","year":"1973","journal-title":"Genetics"},{"issue":"4","key":"2026010907350822000_2026.01.06.697974v1.28","doi-asserted-by":"crossref","first-page":"iyaf029","DOI":"10.1093\/genetics\/iyaf029","article-title":"Suppression of meiotic crossovers in pericentromeric heterochromatin requires synaptonemal complex and meiotic recombination factors in Drosophila melanogaster","volume":"229","year":"2025","journal-title":"Genetics"},{"key":"2026010907350822000_2026.01.06.697974v1.29","doi-asserted-by":"crossref","unstructured":"Pazhayam NM , Turcotte CA , Sekelsky J. 2021. Meiotic crossover patterning. Frontiers in Cell and Developmental Biology 9.","DOI":"10.3389\/fcell.2021.681123"},{"key":"2026010907350822000_2026.01.06.697974v1.30","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/7.6.1011"},{"key":"2026010907350822000_2026.01.06.697974v1.31","doi-asserted-by":"crossref","unstructured":"Sen S , Sarkar S , Menon G , Nambiar M. 2024. Centromere-proximal crossovers disrupt proper homologous chromosome disjunction during meiosis.","DOI":"10.1101\/2024.11.18.624114"},{"key":"2026010907350822000_2026.01.06.697974v1.32","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1400140104"},{"key":"2026010907350822000_2026.01.06.697974v1.33","doi-asserted-by":"publisher","DOI":"10.1007\/bf01792906"},{"key":"2026010907350822000_2026.01.06.697974v1.34","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2024.05.002"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2026.01.06.697974","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T05:14:15Z","timestamp":1782278055000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2026.01.06.697974"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,7]]},"references-count":34,"URL":"https:\/\/doi.org\/10.64898\/2026.01.06.697974","relation":{"is-preprint-of":[{"id-type":"doi","id":"10.1093\/genetics\/iyag076","asserted-by":"subject"}]},"subject":[],"published":{"date-parts":[[2026,1,7]]},"subtype":"preprint"}}