{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:11:06Z","timestamp":1785427866910,"version":"3.56.0"},"posted":{"date-parts":[[2026,1,8]]},"group-title":"Molecular Biology","reference-count":14,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"award":["U01HL175444"],"award-info":[{"award-number":["U01HL175444"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01HL145372"],"award-info":[{"award-number":["R01HL145372"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01HG011886"],"award-info":[{"award-number":["R01HG011886"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,1,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Spatial transcriptomics faces a trade-off between the number of genes assayed and depth of per-gene sensitivity. We developed a \u2018dual chemistry\u2019 method that combines the high sensitivity of a 10X Genomics Xenium V1 custom panel (up to 480 genes) with the broad coverage of the Prime 5K panel (5001 genes) on a single tissue section. This involved co-hybridizing Prime and V1 probes and sequentially running the V1 and Prime decoding chemistries. Applied to a human lung tissue microarray, we observed high concordance between the V1 and Prime chemistries when run independently (on serial sections) and the dual chemistry runs. Overlapping genes (profiled on both V1 and Prime chemistries) showed similar expression patterns in the dual run demonstrating the fidelity of the assay. By combining information from both the V1 and Prime chemistries within the same cell, we retain more cells, gain valuable additional information, and enable both high sensitivity profiling and discovery.<\/jats:p>","DOI":"10.64898\/2026.01.07.698201","type":"posted-content","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T21:00:28Z","timestamp":1767906028000},"source":"Crossref","is-referenced-by-count":0,"title":["Fishing with Two Lines: A Hybrid Approach to Spatial Transcriptomic Discovery"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-2725-0221","authenticated-orcid":false,"given":"Arianna L.","family":"Williams-Katek","sequence":"first","affiliation":[{"name":"Division of Bioinnovation and Genome Sciences, Translational Genomics Research Institute (TGen), Phoenix, Arizona, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4188-9608","authenticated-orcid":false,"given":"Saahithi","family":"Mallapragada","sequence":"additional","affiliation":[{"name":"Division of Bioinnovation and Genome Sciences, Translational Genomics Research Institute (TGen), Phoenix, Arizona, United States"},{"name":"School of Life Sciences, Arizona State University, Tempe, AZ, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0919-3155","authenticated-orcid":false,"given":"Evan D.","family":"Mee","sequence":"additional","affiliation":[{"name":"Division of Bioinnovation and Genome Sciences, Translational Genomics Research Institute (TGen), Phoenix, Arizona, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6448-2965","authenticated-orcid":false,"given":"Brandon K.","family":"Fischer","sequence":"additional","affiliation":[{"name":"Division of Bioinnovation and Genome Sciences, Translational Genomics Research Institute (TGen), Phoenix, Arizona, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6606-5366","authenticated-orcid":false,"given":"Laurie C.","family":"Eldredge","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Sleep Medicine, Department of Pediatrics, University of Washington School of Medicine, Seattle, Washington, United States"},{"name":"Seattle Children\u2019s Hospital, Seattle, Washington, United States"},{"name":"Center for Respiratory Biology and Therapeutics, Seattle Children\u2019s Research Institute, Seattle, Washington, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0571-0285","authenticated-orcid":false,"given":"Gail H.","family":"Deutsch","sequence":"additional","affiliation":[{"name":"Seattle Children\u2019s Hospital, Seattle, Washington, United States"},{"name":"Center for Respiratory Biology and Therapeutics, Seattle Children\u2019s Research Institute, Seattle, Washington, United States"},{"name":"Department of Laboratory Medicine and Pathology, University of Washington and Seattle Children\u2019s Hospital, Seattle, Washington, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8923-1344","authenticated-orcid":false,"given":"Jonathan A.","family":"Kropski","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology, Vanderbilt University, Nashville, Tennessee, United States"},{"name":"Division of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, United States"},{"name":"Department of Veterans Affairs Medical Center, Nashville, Tennessee, United States"},{"name":"Biodevelopmental Origins of Lung Disease (BOLD) Center, Vanderbilt University School of Medicine, Nashville, Tennessee, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6613-1439","authenticated-orcid":false,"given":"Jennifer M. 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