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Transmitted through ticks and caused by the bacterium\n                  <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                  (\n                  <jats:italic>Bb<\/jats:italic>\n                  ), Lyme disease (LD) is the most common vector-borne disease in the Northern hemisphere. There are no effective vaccines, making it suitable for testing the concept of rational adjuvant selection. Here, we formulated our previously developed and effective LD vaccine antigen, CspZ-YA\n                  <jats:sub>C187S,<\/jats:sub>\n                  with different adjuvants suitable for human use; we analyzed the immune response by transcriptomics and tested the vaccine efficacy after\n                  <jats:italic>Bb<\/jats:italic>\n                  infection. We identified Alum-CpG and Alum-\u03b1Gal to elicit the highest titers of CspZ-YA\n                  <jats:sub>C187S<\/jats:sub>\n                  -dependent protective antibodies and robust levels of protection but through distinct mechanisms of immunomodulation. We demonstrated that immunization with Alum-CpG formulated CspZ-YA\n                  <jats:sub>C187S<\/jats:sub>\n                  provided up to nine months of protective bactericidal antibody titers, as well as recall-memory response to prevent LD after natural infection. Immunity was linked to elevated levels of IgG1 memory cells in the vaccine-triggered immune responses. This work thus identified a durable, memory immunity-driven LD vaccine, ultimately paving the road to understanding the mechanisms of rationale adjuvant selection for vaccine development.\n                <\/jats:p>","DOI":"10.64898\/2026.01.08.698480","type":"posted-content","created":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T02:50:18Z","timestamp":1768013418000},"source":"Crossref","is-referenced-by-count":1,"title":["Develop a durable, memory-driven, CspZ-targeting Lyme disease vaccine by rationale adjuvant selection"],"prefix":"10.64898","author":[{"given":"Miranda","family":"McCarty","sequence":"first","affiliation":[{"name":"Department of Infectious Disease and Global Health, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio A.","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease and Global Health, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA, USA"},{"name":"Department of Molecular Biology and Microbiology, School of Medicine, Tufts University, Boston; MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jill","family":"Malfetano","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Wadsworth Center, NYSDOH, Albany, NY, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Carolina","family":"Le\u00e3o","sequence":"additional","affiliation":[{"name":"Department of Pediatrics (Tropical Medicine), National School of Tropical Medicine, Baylor College of Medicine, Houston, TX, USA"},{"name":"Texas Children\u2019s Hospital Center for Vaccine Development, Houston, TX, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Jose","family":"Villar","sequence":"additional","affiliation":[{"name":"Department of Pediatrics (Tropical Medicine), National School of Tropical Medicine, Baylor College of Medicine, Houston, TX, USA"},{"name":"Texas Children\u2019s Hospital Center for Vaccine Development, Houston, TX, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuli","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi-Lin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pediatrics (Tropical Medicine), National School of Tropical Medicine, Baylor College of Medicine, Houston, TX, USA"},{"name":"Texas Children\u2019s Hospital Center for Vaccine Development, Houston, TX, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jungsoon","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Pediatrics (Tropical Medicine), National School of Tropical Medicine, Baylor College of Medicine, Houston, TX, USA"},{"name":"Texas Children\u2019s Hospital Center for Vaccine Development, Houston, TX, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuyun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics (Tropical Medicine), National School of Tropical Medicine, Baylor College of Medicine, Houston, TX, USA"},{"name":"Texas Children\u2019s Hospital Center for Vaccine Development, Houston, TX, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Strych","sequence":"additional","affiliation":[{"name":"Department of Pediatrics (Tropical Medicine), National School of Tropical Medicine, Baylor College of Medicine, Houston, TX, USA"},{"name":"Texas Children\u2019s Hospital Center for Vaccine Development, Houston, TX, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Elena","family":"Bottazzi","sequence":"additional","affiliation":[{"name":"Department of Pediatrics (Tropical Medicine), National School of Tropical Medicine, Baylor College of Medicine, Houston, TX, USA"},{"name":"Texas Children\u2019s Hospital Center for Vaccine Development, Houston, TX, USA"},{"name":"Department of Biology, Baylor University, Waco, TX, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Utpal","family":"Pal","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klemen","family":"Strle","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Microbiology, School of Medicine, Tufts University, Boston; 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