{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:09:08Z","timestamp":1785409748557,"version":"3.56.0"},"posted":{"date-parts":[[2026,1,9]]},"group-title":"Genomics","reference-count":78,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:00:00Z","timestamp":1767916800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"id":[{"id":"https:\/\/ror.org\/036jqmy94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"NIH","award":["R35 GM058939-19S1"],"award-info":[{"award-number":["R35 GM058939-19S1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,1,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Organisms that thrive in extreme environments provide natural experiments in evolution, revealing the genetic regulators that orchestrate complex phenotypic change. Wine yeast are specialized strains that are adapted to survive in the wine making environment while producing high concentrations of ethanol. In addition to large genomic changes that differentiate wine yeast from yeast used in other industries, single nucleotide and polyglutamine tract polymorphisms in the transcriptional regulator Med15 are associated with the fermentation efficiency and stress response phenotypes of wine yeast. In this study we investigated the transcriptional differences during wine fermentation in transgenic lab strain yeast having integrated wine yeast\n                  <jats:italic>MED15<\/jats:italic>\n                  alleles. Compared to the unmodified lab strain (LAB or\n                  <jats:italic>MED15<\/jats:italic>\n                  <jats:sub>LAB<\/jats:sub>\n                  ), the same strain in which the\n                  <jats:italic>MED15<\/jats:italic>\n                  locus was replaced with a\n                  <jats:italic>MED15<\/jats:italic>\n                  allele from yeast isolated from palm wine, the fermented sap of palm (oil, date, coconut) trees, (WY23, or\n                  <jats:italic>MED15<\/jats:italic>\n                  <jats:sub>WY23<\/jats:sub>\n                  ) exhibited enhanced expression of glycolytic, fermentation, and amino acid biosynthesis genes. Our experimental data confirms the importance of arginine biosynthetic genes during the fermentation process and suggests that the improvement in fermentation efficiency in strains with\n                  <jats:italic>MED15<\/jats:italic>\n                  alleles from some wine yeast strains may be related to the role of Med15 in expression of the genes of the arginine biosynthetic pathway. The global benefit conferred by polymorphisms in a single transcriptional regulator, makes Med15 a prime target for engineering of strains devoted to various types of alcohol production.\n                <\/jats:p>","DOI":"10.64898\/2026.01.08.698492","type":"posted-content","created":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T03:05:15Z","timestamp":1768014315000},"source":"Crossref","is-referenced-by-count":0,"title":["Transcriptomic shift in ethanol and amino acid metabolic genes regulated by Med15 during alcoholic fermentation"],"prefix":"10.64898","author":[{"given":"David G.","family":"Cooper","sequence":"first","affiliation":[{"name":"Department of Biology, University of Iowa, Iowa City, IA"},{"name":"Department of Pharmaceutical Sciences, Butler University, Indianapolis, IN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2756-1455","authenticated-orcid":false,"given":"Emma","family":"Grunkemeyer","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Iowa, Iowa City, IA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8281-6024","authenticated-orcid":false,"given":"Jan S.","family":"Fassler","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Iowa, Iowa City, IA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026011100450447000_2026.01.08.698492v1.1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.741572"},{"key":"2026011100450447000_2026.01.08.698492v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2019.03.008"},{"key":"2026011100450447000_2026.01.08.698492v1.3","doi-asserted-by":"publisher","DOI":"10.1093\/femsyr\/fov067"},{"key":"2026011100450447000_2026.01.08.698492v1.4","doi-asserted-by":"publisher","DOI":"10.1101\/gr.10.4.403"},{"key":"2026011100450447000_2026.01.08.698492v1.5","unstructured":"Fleet GH . 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