{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T19:35:16Z","timestamp":1768332916097,"version":"3.49.0"},"posted":{"date-parts":[[2026,1,10]]},"group-title":"Developmental Biology","reference-count":81,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T00:00:00Z","timestamp":1768003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"crossref","award":["ANR-24-CE14-3347"],"award-info":[{"award-number":["ANR-24-CE14-3347"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100015326","name":"NIH Common Fund","doi-asserted-by":"crossref","award":["R01 EY021505"],"award-info":[{"award-number":["R01 EY021505"]}],"id":[{"id":"10.13039\/100015326","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100015326","name":"NIH Common Fund","doi-asserted-by":"crossref","award":["R01 EY036923"],"award-info":[{"award-number":["R01 EY036923"]}],"id":[{"id":"10.13039\/100015326","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,1,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Precise post-transcriptional regulation of gene expression is essential for vertebrate lens development. Disruption of the gene encoding the RNA-binding protein CELF1 leads to early-onset cataract in mice. Here, using iCLIP-seq in lenses, we mapped transcriptome-wide CELF1 binding sites, revealing interactions with the 3\u2032UTRs of key transcripts involved in lens development and pathology like\n                  <jats:italic>Gja8<\/jats:italic>\n                  ,\n                  <jats:italic>Jag1<\/jats:italic>\n                  ,\n                  <jats:italic>Maf<\/jats:italic>\n                  ,\n                  <jats:italic>Pax6<\/jats:italic>\n                  , or\n                  <jats:italic>Prox1<\/jats:italic>\n                  . Integrated analysis with transcriptomic data and luciferase reporter assays demonstrated that binding of CELF1 protein represses its target mRNAs by destabilizing transcripts and\/or inhibiting their translation. Indeed, the cataract-linked genes\n                  <jats:italic>Maf<\/jats:italic>\n                  and\n                  <jats:italic>Gja8<\/jats:italic>\n                  are upregulated in\n                  <jats:italic>Celf1<\/jats:italic>\n                  <jats:sup>cKO<\/jats:sup>\n                  lenses. In\n                  <jats:italic>Xenopus laevis<\/jats:italic>\n                  , overexpression of\n                  <jats:italic>maf<\/jats:italic>\n                  resulted in abnormal lens structure and eye morphology, confirming the developmental relevance of CELF1-mediated repression. Our findings uncover a post-transcriptional network in which CELF1 controls lens morphogenesis by limiting the expression of critical genes at the mRNA level to achive their proper dosage.\n                <\/jats:p>","DOI":"10.64898\/2026.01.09.698617","type":"posted-content","created":{"date-parts":[[2026,1,11]],"date-time":"2026-01-11T04:25:11Z","timestamp":1768105511000},"source":"Crossref","is-referenced-by-count":0,"title":["Mapping of CELF1-RNA interactions reveals post-transcriptional control of lens development"],"prefix":"10.64898","author":[{"given":"Justine","family":"Viet","sequence":"first","affiliation":[{"name":"Univ Rennes, CNRS, IGDR (Institut de G\u00e9n\u00e9tique et D\u00e9veloppement de Rennes), UMR 6290, F-35000 Rennes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4255-6093","authenticated-orcid":false,"given":"Matthieu","family":"Duot","sequence":"additional","affiliation":[{"name":"Univ Rennes, CNRS, 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