{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T06:29:21Z","timestamp":1793687361456,"version":"4.5.1"},"posted":{"date-parts":[[2026,1,12]]},"group-title":"Neuroscience","reference-count":0,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"HHMI Hanna Gray Fellowship"},{"name":"NIH","award":["MIRA R35 GM146960"],"award-info":[{"award-number":["MIRA R35 GM146960"]}]},{"name":"NIH","award":["NIGMS-T32-GM149363-02"],"award-info":[{"award-number":["NIGMS-T32-GM149363-02"]}]},{"name":"NIH","award":["1R01 AT011447-01"],"award-info":[{"award-number":["1R01 AT011447-01"]}]},{"name":"NIH","award":["R35 5R35NS097344-05"],"award-info":[{"award-number":["R35 5R35NS097344-05"]}]},{"name":"NIH","award":["R35 5R35NS132196-02"],"award-info":[{"award-number":["R35 5R35NS132196-02"]}]},{"name":"Vanderbilt Seeding Success Grant","award":["FF_300627"],"award-info":[{"award-number":["FF_300627"]}]},{"name":"Vanderbilt CCSB Accelerator Fund"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,11,2]]},"abstract":"<jats:p>The spinal cord integrates diverse somatosensory inputs and executes motor outputs through anatomically and functionally distinct circuits. Here, we employed spatially resolved single-cell transcriptomic profiling of the adult mouse spinal cord to gain insights into the organizational logic of the spinal cord. Our results reveal distinct spatial and laminar distributions of neuronal subtypes, including axial level and sex-specific differences. Many neuronal subtypes exhibit close spatial proximity, implicating regionally specific patterns of connectivity and circuit functions. Additionally, neuronal subtypes within the dorsal horn exhibit a wide range of predicted cell-cell communication motifs, as assessed by the spatial distribution of neuropeptide- and other ligand-receptor pairs. Finally, we identified several neuronal subtypes with altered transcriptomic and predicted cell-cell communications in a model of neuropathic pain. This spatially resolved cellular and molecular map of the spinal cord will facilitate the decoding of circuit mechanisms underlying somatosensory and motor functions in health and disease.<\/jats:p>","DOI":"10.64898\/2026.01.10.698734","type":"posted-content","created":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T16:20:12Z","timestamp":1768234812000},"source":"Crossref","is-referenced-by-count":8,"title":["Spatial transcriptomics reveals organizational properties of the mouse spinal cord and alterations in neuropathic pain"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6453-7358","authenticated-orcid":false,"given":"Qimeng","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Vanderbilt University, Nashville, TN 37240, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunfei","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Computer Science, Vanderbilt University, Nashville, TN 37235, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuling","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 37235, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuella","family":"Osei-Asante","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gautham","family":"Chelliah","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Vanderbilt University, Nashville, TN 37240, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Sobanko","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Vanderbilt University, Nashville, TN 37240, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brianna","family":"Sanchez","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9723-8530","authenticated-orcid":false,"given":"David D","family":"Ginty","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin Maizie","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 37235, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7927-7253","authenticated-orcid":false,"given":"Shan","family":"Meltzer","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Vanderbilt University, Nashville, TN 37240, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2026.01.10.698734","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T06:20:11Z","timestamp":1793686811000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2026.01.10.698734"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,12]]},"references-count":0,"URL":"https:\/\/doi.org\/10.64898\/2026.01.10.698734","relation":{},"subject":[],"published":{"date-parts":[[2026,1,12]]},"subtype":"preprint"}}