{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:50:08Z","timestamp":1785408608739,"version":"3.56.0"},"posted":{"date-parts":[[2026,1,12]]},"group-title":"Microbiology","reference-count":89,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"award":["5P30GM145393"],"award-info":[{"award-number":["5P30GM145393"]}],"id":[{"id":"https:\/\/ror.org\/04q48ey07","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Center for Microbial Pathogenesis and Host Inflammatory Responses, Arkansas, US"},{"award":["BX006469"],"award-info":[{"award-number":["BX006469"]}],"id":[{"id":"https:\/\/ror.org\/05rsv9s98","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01AI119380"],"award-info":[{"award-number":["R01AI119380"]}],"id":[{"id":"https:\/\/ror.org\/043z4tv69","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,1,12]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                <jats:p>\n                  <jats:italic>Staphylococcus aureus<\/jats:italic>\n                  secretes lipases that hydrolyze glycerol esters to mitigate host detection and scavenge fatty acids for membrane synthesis, but exposure to host-derived polyunsaturated fatty acids (PUFA) can be toxic. Here, we show that host hyperlipidemia protects against\n                  <jats:italic>S. aureus<\/jats:italic>\n                  osteomyelitis by engaging this vulnerability. Using a mouse model of severe hyperlipidemia, we show that PUFA-enriched plasma triacylglycerides are associated with reduced bacterial burden and bone destruction from injury-associated osteomyelitis.\n                  <jats:italic>In vitro<\/jats:italic>\n                  , human triacylglyceride-rich lipoproteins (TRL) exhibit potent bactericidal activity against post-exponential\n                  <jats:italic>S. aureus<\/jats:italic>\n                  that requires lipase activity of glycerol ester hydrolase (\n                  <jats:italic>geh<\/jats:italic>\n                  ). Pre-exponential exposure to TRL suppressed\n                  <jats:italic>agr<\/jats:italic>\n                  quorum-sensing, blunting\n                  <jats:italic>geh<\/jats:italic>\n                  expression and toxicity; however, bactericidal activity of TRL could be restored by exogenous lipoprotein lipase. Together, these findings reveal TRL as active antimicrobial particles whose toxicity is unmasked by lipolysis and suggest that modulation of host lipid metabolism may provide new therapeutic opportunities for chronic\n                  <jats:italic>S. aureus<\/jats:italic>\n                  infection.\n                <\/jats:p>","DOI":"10.64898\/2026.01.12.699058","type":"posted-content","created":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T05:45:34Z","timestamp":1768283134000},"source":"Crossref","is-referenced-by-count":0,"title":["Lipolysis of host triacylglyceride-rich lipoproteins creates a toxic microenvironment for\n                  <i>Staphylococcus aureus<\/i>"],"prefix":"10.64898","author":[{"given":"Trushali","family":"Vansadia","sequence":"first","affiliation":[{"name":"Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camron N.","family":"Collins","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anisha","family":"Neupane","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathaniel J.","family":"Torres","sequence":"additional","affiliation":[{"name":"University of Arkansas for Medical Sciences, Department of Microbiology and Immunology, Little Rock, AR 72205"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahsan","family":"Hameed","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205; Arkansas Children\u2019s Nutrition Research Center, Little Rock, AR 72205"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Morris","sequence":"additional","affiliation":[{"name":"Arkansas Children\u2019s Nutrition Research Center and Central Arkansas Veterans Affairs Healthcare System, Little Rock, AR 72205"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen E.","family":"Beenken","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR 72205; Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark S.","family":"Smeltzer","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR 72205; Department of Orthopaedic Surgery, University of Arkansas for Medical Sciences, Little Rock, AR 72205"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8057-3436","authenticated-orcid":false,"given":"Ryan M.","family":"Allen","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205; Winthrop P. 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