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Here we report on an alternative gene-editing approach using small numbers of hematopoietic stem and progenitor cells (HSPCs) to direct long-term, high-level expression of antibodies or cargo proteins. Edited B lymphocyte offspring can be activated by cognate antigen to undergo clonal expansion and develop into specific antibody or cargo protein-synthesizing plasma cells. These cells produce long-lasting, therapeutic levels of serum antibody against HIV-1 or malaria and an anti-influenza virus bNAb that mediated universal protection from heterologous lethal challenge. Our data provide a paradigm for cell therapy approaches to prevent or treat disease using self-amplifying B cell protein factories.\n                <\/jats:p>","DOI":"10.64898\/2026.01.16.699998","type":"posted-content","created":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T20:20:15Z","timestamp":1768767615000},"source":"Crossref","is-referenced-by-count":0,"title":["B Lymphocyte Protein Factories produced by Hematopoietic Stem Cell Gene Editing"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1118-1818","authenticated-orcid":false,"given":"Harald","family":"Hartweger","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Immunology, Rockefeller University; New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Ruprecht","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Rockefeller University; New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai-Hui","family":"Yao","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Rockefeller University; New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6977-7009","authenticated-orcid":false,"given":"Philippe","family":"Laffont","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Rockefeller University; New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7511-1379","authenticated-orcid":false,"given":"Gabriella","family":"Lima Dos Reis","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Rockefeller University; New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengcheng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Rockefeller University; New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7751-0928","authenticated-orcid":false,"given":"Thomas","family":"H\u00e4ggl\u00f6f","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Rockefeller University; 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