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Robust protection of the balance between stem cell quiescence and proliferation (Q-&gt;P) is critical for long-term tissue health. Proliferation without proper resources drives disease states including cancer or birth defects. Conversely, extended periods of quiescence can lead to irreversible senescence, causing stem cell loss, aging symptoms, and vulnerability to oncogenic transformation. Diet is a central regulator of Q-&gt;P. Tissue stem cells are particularly impacted, entering periods of quiescence during nutrient restriction, with rapid induction of proliferation upon feeding. We demonstrated previously that the Hedgehog (Hh) signaling pathway is necessary and sufficient for controlling Q-&gt;P responses to dietary changes in epithelial Follicle Stem Cells (FSCs) in the fly ovary. The Hh effector, Cubitus Interruptus (Ci), is a transcriptional regulator that mediates the feeding response. To identify Ci-induced Q-&gt;P regulators, we labeled transcripts that are induced in FSCs during the 6-hour Q-&gt;P timecourse using thiouracil tagging (TU-tagging), and sequenced TU-tagged messages versus Input to prioritize candidates. Unexpectedly, cell cycle regulators were not induced, suggesting that other mechanisms control Q-&gt;P in FSCs. We describe a sequential screening approach that uncovered seven novel, feeding-dependent Q-&gt;P regulators, including a cholesterol transporter and, surprisingly, glial and neuronal regulators. Our results highlight the importance of dynamic regulation of gene expression for translation of dietary signals by stem cells, uncovering new pathways for mechanistic investigation.<\/jats:p>","DOI":"10.64898\/2026.01.22.701111","type":"posted-content","created":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T05:50:12Z","timestamp":1769406612000},"source":"Crossref","is-referenced-by-count":1,"title":["Starve-Feed Cycles Direct Quiescence to Proliferation Transitions in\n                  <i>Drosophila<\/i>\n                  Follicle Stem Cells via Transcriptional Regulation"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0335-6956","authenticated-orcid":false,"given":"Eric H.","family":"Lee","sequence":"first","affiliation":[{"name":"Fox Chase Cancer Center, Program in Cancer Signaling and Microenvironment, Philadelphia, 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