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Two of these, the\n                  <jats:italic>las<\/jats:italic>\n                  and\n                  <jats:italic>rhl<\/jats:italic>\n                  systems, involve acyl-homoserine lactone signals; the third, called\n                  <jats:italic>pqs<\/jats:italic>\n                  , primarily uses the signal 2-heptyl-3-hydroxy-4(1\n                  <jats:italic>H<\/jats:italic>\n                  )-quinolone (\u201cPQS\u201d). We aimed to identify how interbacterial interactions are regulated between\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  and\n                  <jats:italic>Stenotrophomonas maltophilia<\/jats:italic>\n                  , which co-occur in many environments, including the airways of people with cystic fibrosis. We explored\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  and\n                  <jats:italic>S. maltophilia<\/jats:italic>\n                  interactions using a co-culture model. In the conditions of our experiments,\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  kills\n                  <jats:italic>S. maltophilia<\/jats:italic>\n                  . Co-culture of\n                  <jats:italic>S. maltophilia<\/jats:italic>\n                  with\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  deficient in\n                  <jats:italic>las<\/jats:italic>\n                  ,\n                  <jats:italic>rhl<\/jats:italic>\n                  , or\n                  <jats:italic>pqs<\/jats:italic>\n                  QS resulted in greater\n                  <jats:italic>S. maltophilia<\/jats:italic>\n                  viability than co-culture with the wildtype. This inhibition was not generally attributable to\n                  <jats:italic>las<\/jats:italic>\n                  and\n                  <jats:italic>rhl<\/jats:italic>\n                  -regulated toxins. Therefore, we interrogated the role of\n                  <jats:italic>pqs<\/jats:italic>\n                  QS and found that co-culture of\n                  <jats:italic>S. maltophilia<\/jats:italic>\n                  with\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  deficient in PQS biosynthesis showed similar CFUs to monoculture. Exogenous PQS did not complement this phenotype, suggesting that another quinolone is the effector. We found that\n                  <jats:italic>S. maltophilia<\/jats:italic>\n                  killing is reduced in competition with a mutant that cannot make the quinolone HQNO (2-heptyl-4-quinoline\n                  <jats:italic>N<\/jats:italic>\n                  -oxide). We show that full killing of\n                  <jats:italic>S. maltophilia<\/jats:italic>\n                  by\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  requires three components: HQNO, the chaperone PqsE, and intact PQS biosynthesis that together form a tripartite AND logic gate. Our work identifies quinolone quorum sensing as a driver for interactions between the Gram-negative pathogens\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  and\n                  <jats:italic>S. maltophilia<\/jats:italic>\n                  .\n                <\/jats:p>","DOI":"10.64898\/2026.01.27.702039","type":"posted-content","created":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T03:10:11Z","timestamp":1769569811000},"source":"Crossref","is-referenced-by-count":0,"title":["A three-component logic gate governs quorum sensing-regulated killing of\n                  <i>Stenotrophomonas maltophilia<\/i>\n                  by\n                  <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3476-1345","authenticated-orcid":false,"given":"Andrew","family":"Frando","sequence":"first","affiliation":[{"name":"Departments of Medicine, University of Washington, Seattle, WA 98195 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert S.","family":"Parsek","sequence":"additional","affiliation":[{"name":"Microbiology, University of Washington, Seattle, WA 98195 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graham W.","family":"Roberts","sequence":"additional","affiliation":[{"name":"Microbiology, University of Washington, Seattle, WA 98195 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2992-6523","authenticated-orcid":false,"given":"Ajai A.","family":"Dandekar","sequence":"additional","affiliation":[{"name":"Departments of Medicine, University of Washington, Seattle, WA 98195 USA"},{"name":"Microbiology, University of Washington, Seattle, WA 98195 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026051503401301000_2026.01.27.702039v2.1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.87"},{"key":"2026051503401301000_2026.01.27.702039v2.2","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-09550-9_2"},{"key":"2026051503401301000_2026.01.27.702039v2.3","doi-asserted-by":"publisher","DOI":"10.1016\/J.MIB.2017.06.006"},{"key":"2026051503401301000_2026.01.27.702039v2.4","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.13226"},{"key":"2026051503401301000_2026.01.27.702039v2.5","doi-asserted-by":"publisher","DOI":"10.20517\/mrr.2023.10"},{"key":"2026051503401301000_2026.01.27.702039v2.6","doi-asserted-by":"publisher","DOI":"10.1038\/nature23292"},{"key":"2026051503401301000_2026.01.27.702039v2.7","doi-asserted-by":"publisher","DOI":"10.1017\/ice.2015.168"},{"key":"2026051503401301000_2026.01.27.702039v2.8","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.1.57-71.2004"},{"key":"2026051503401301000_2026.01.27.702039v2.9","doi-asserted-by":"publisher","DOI":"10.1017\/s0022172400046271"},{"key":"2026051503401301000_2026.01.27.702039v2.10","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.28.6.987-991.1974"},{"key":"2026051503401301000_2026.01.27.702039v2.11","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-143-11-200512060-00010"},{"key":"2026051503401301000_2026.01.27.702039v2.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.eimc.2015.05.006"},{"issue":"1","key":"2026051503401301000_2026.01.27.702039v2.13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40348-016-0067-1","article-title":"Acquisition and adaptation of the airway microbiota in the early life of cystic fibrosis patients","volume":"4","year":"2017","journal-title":"Mol Cell Pediatr"},{"key":"2026051503401301000_2026.01.27.702039v2.14","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-00477-5"},{"key":"2026051503401301000_2026.01.27.702039v2.15","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msaa226"},{"key":"2026051503401301000_2026.01.27.702039v2.16","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/30.5.615"},{"key":"2026051503401301000_2026.01.27.702039v2.17","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01211-09"},{"key":"2026051503401301000_2026.01.27.702039v2.18","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.2.554-566.2005"},{"key":"2026051503401301000_2026.01.27.702039v2.19","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00482-15"},{"key":"2026051503401301000_2026.01.27.702039v2.20","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0511323103"},{"key":"2026051503401301000_2026.01.27.702039v2.21","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.074203-0"},{"key":"2026051503401301000_2026.01.27.702039v2.22","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00059-15"},{"key":"2026051503401301000_2026.01.27.702039v2.23","doi-asserted-by":"publisher","DOI":"10.1111\/cei.13329"},{"key":"2026051503401301000_2026.01.27.702039v2.24","doi-asserted-by":"crossref","unstructured":"Pajon C , Fortoul MC , Diaz-Tang G , Marin Meneses E , Kalifa AR , Sevy E , et al. 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