{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T10:42:32Z","timestamp":1785494552142,"version":"3.56.0"},"posted":{"date-parts":[[2026,2,1]]},"group-title":"Neuroscience","reference-count":52,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"NIH","award":["NS078152"],"award-info":[{"award-number":["NS078152"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Large-conductance Ca\n                  <jats:sup>2+<\/jats:sup>\n                  - and voltage-activated K\n                  <jats:sup>+<\/jats:sup>\n                  (BK) channels are widely expressed, including in the brain where they shape neuronal excitability. Their physiological functions are strongly influenced by cell-type-specific auxiliary subunits. The auxiliary \u03b33 subunit (LRRC55) enhances BK-channel activation by shifting voltage-dependent gating toward more negative potentials; however, its protein distribution and in vivo function remain unclear. Here, we generated knock-in mice carrying a C-terminal epitope tag on endogenous LRRC55 to map its expression, and\n                  <jats:italic>Lrrc55<\/jats:italic>\n                  knockout mice to test its function. LRRC55 protein was selectively enriched in cerebellar Purkinje cells.\n                  <jats:italic>Lrrc55<\/jats:italic>\n                  deletion produced ataxia-like impairments in gait, balance, and coordination. In acute slices, pharmacological BK-channel block with paxilline altered Purkinje cell simple- and complex-spike firing in wild-type mice, whereas these BK-dependent effects were largely absent in\n                  <jats:italic>Lrrc55<\/jats:italic>\n                  knockouts, indicating that LRRC55 is required for BK channels to shape Purkinje cell firing under these conditions. Moreover, LRRC55 loss disrupted cerebellar synaptic plasticity, abolishing parallel fiber\u2013Purkinje cell long-term potentiation and eliminating climbing fiber\u2013Purkinje cell long-term depression, phenocopying paxilline in wild-type cells. Together, these results identify LRRC55 as a Purkinje-cell-enriched auxiliary subunit that is essential for BK-dependent excitability and plasticity and that supports normal cerebellar motor function.\n                <\/jats:p>","DOI":"10.64898\/2026.01.30.702921","type":"posted-content","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T07:15:23Z","timestamp":1770016523000},"source":"Crossref","is-referenced-by-count":1,"title":["LRRC55 modulates BK channels to support Purkinje cell plasticity and motor coordination"],"prefix":"10.64898","author":[{"given":"Xin","family":"Guan","sequence":"first","affiliation":[{"name":"Department of Anesthesiology & Perioperative Medicine, The University of Texas MD Anderson Cancer Center, Houston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiusheng","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology & Perioperative Medicine, The University of Texas MD Anderson Cancer Center, Houston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026040409051507000_2026.01.30.702921v3.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2010.09.015"},{"key":"2026040409051507000_2026.01.30.702921v3.2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00020.2009"},{"key":"2026040409051507000_2026.01.30.702921v3.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2005.10.007"},{"key":"2026040409051507000_2026.01.30.702921v3.4","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1992"},{"key":"2026040409051507000_2026.01.30.702921v3.5","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1992"},{"key":"2026040409051507000_2026.01.30.702921v3.6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.00135.x"},{"key":"2026040409051507000_2026.01.30.702921v3.7","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.2002.02171.x"},{"key":"2026040409051507000_2026.01.30.702921v3.8","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0192-20.2021"},{"key":"2026040409051507000_2026.01.30.702921v3.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2009.05.016"},{"key":"2026040409051507000_2026.01.30.702921v3.10","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-20-08789.1999"},{"key":"2026040409051507000_2026.01.30.702921v3.11","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.062661"},{"key":"2026040409051507000_2026.01.30.702921v3.12","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-24-09585.2001"},{"key":"2026040409051507000_2026.01.30.702921v3.13","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-014-1287-1"},{"key":"2026040409051507000_2026.01.30.702921v3.14","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1572-05.2005"},{"key":"2026040409051507000_2026.01.30.702921v3.15","doi-asserted-by":"crossref","unstructured":"X. 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