{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"medRxiv"}],"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T21:19:51Z","timestamp":1788556791142,"version":"build-2803163510"},"posted":{"date-parts":[[2026,2,4]]},"group-title":"Transplantation","reference-count":37,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,2,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Kidney transplantation faces a critical paradox: while thousands await organs, approximately 30% of potential deceased donor kidneys are discarded for various reasons, including\n                  <jats:italic>subjective<\/jats:italic>\n                  assessments due to the lack of an objective molecular biomarker of preservation quality. Here, we applied novel \u201ctop-down\u201d proteoform imaging mass spectrometry across living donor (LD), deceased donor (brain death or cardiac death), and discarded human kidneys to quantify proteoforms correlating with post-transplant kidney function. This approach preserves post-translational modifications and splice variants, revealing molecular tissue variability beyond protein presence.\n                <\/jats:p>\n                <jats:p>LD kidneys displayed robust metabolic signatures, including L-xylulose reductase and cytochrome oxidase subunits, whereas deceased donor and discarded organs showed elevated cellular stress markers such as alpha-B-crystallin and peroxiredoxin 1. Post-transplant blood proteoform analysis validated tissue findings, demonstrating persistent cellular stress and immune activation in deceased donor recipients compared with physiologic wound healing in LD recipients. Consistent with these molecular predictions, serum creatinine levels were highest in DCD, intermediate in DBD, and lowest in LD recipients.<\/jats:p>\n                <jats:p>\n                  The intersection of tissue proteoform signatures across all marginal tissues identified four proteoforms consistently elevated in deceased and discarded kidneys: ACTG1, acetylated CRYAB, PARK7, and S100A4. Collectively, these proteoforms capture key molecular indicators of graft quality, reflecting oxidative stress, cellular injury, and immune activation pathways. As such, they represent promising point-of-care (POC) biomarker candidates for\n                  <jats:italic>objective<\/jats:italic>\n                  kidney classification, potentially improving donor kidney utilization.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Translational statement<\/jats:title>\n                  <jats:p>Current methods for evaluating donor kidney quality rely on subjective assessments, contributing to the discard of approximately 30% of potentially viable organs. This study demonstrates that \u201ctop-down\u201d proteomics can objectively identify molecular signatures distinguishing high-quality from marginal donor kidneys. Top-down proteomics analyzes intact proteins with their post-translational modifications or cleavage products, termed proteoforms to provide mechanistic insights into graft quality. We identified four proteoforms (ACTG1, acetylated CRYAB, PARK7, and S100A4) to be consistently elevated in deceased and discarded kidneys, reflecting oxidative stress, cellular injury, and immune activation. These molecular markers correlated with post-transplant kidney outcome, as measured by serum creatinine levels and recipient blood proteoforms. As a next step, validation in larger cohorts could establish these proteoforms as point-of-care biomarkers for real-time donor kidney assessment during procurement. This objective molecular stratification could reduce unnecessary organ discards and improve transplant outcomes by matching organ quality with recipient risk profiles.<\/jats:p>\n                <\/jats:sec>","DOI":"10.64898\/2026.02.02.26345404","type":"posted-content","created":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T01:40:54Z","timestamp":1770255654000},"source":"Crossref","is-referenced-by-count":1,"title":["Top-Down Proteomics in the Assessment of Kidney Donor Quality: a novel approach to increased organ utilization"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6886-8936","authenticated-orcid":false,"given":"Claudia","family":"Ctortecka","sequence":"first","affiliation":[{"name":"Chemistry of Life Processes Institute, Northwestern University, Evanston, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dinesh","family":"Jaishankar","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Organ Transplant, Feinberg School of Medicine, Northwestern University, Chicago, IL"},{"name":"Comprehensive Transplant Center, Feinberg School of Medicine, Northwestern University, Chicago, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Su","sequence":"additional","affiliation":[{"name":"Proteomics Center of Excellence, Northwestern University, Evanston, IL"},{"name":"Department of Chemistry, Northwestern University, Evanston, IL"},{"name":"Department of Biochemistry, University of California, Riverside, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5799-1533","authenticated-orcid":false,"given":"Che-Fan","family":"Huang","sequence":"additional","affiliation":[{"name":"Proteomics Center of Excellence, Northwestern University, Evanston, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Indira","family":"Pla","sequence":"additional","affiliation":[{"name":"Proteomics Center of Excellence, Northwestern University, Evanston, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathaniel","family":"Henning","sequence":"additional","affiliation":[{"name":"Proteomics Center of Excellence, Northwestern University, Evanston, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A. R.","family":"Hollas","sequence":"additional","affiliation":[{"name":"Proteomics Center of Excellence, Northwestern University, Evanston, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle A.","family":"Callegari","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Organ Transplant, Feinberg School of Medicine, Northwestern University, Chicago, IL"},{"name":"Comprehensive Transplant Center, Feinberg School of Medicine, Northwestern University, Chicago, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meredith E.","family":"Taylor","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Organ Transplant, Feinberg School of Medicine, Northwestern University, Chicago, IL"},{"name":"Comprehensive Transplant Center, Feinberg School of Medicine, Northwestern University, Chicago, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu Min","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Organ Transplant, Feinberg School of Medicine, Northwestern University, Chicago, IL"},{"name":"Comprehensive Transplant Center, Feinberg School of Medicine, Northwestern University, Chicago, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amna","family":"Daud","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Organ Transplant, Feinberg School of Medicine, Northwestern University, Chicago, IL"},{"name":"Comprehensive Transplant Center, Feinberg School of Medicine, Northwestern University, Chicago, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David F.","family":"Pinelli","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Organ Transplant, Feinberg School of Medicine, Northwestern University, Chicago, IL"},{"name":"Comprehensive Transplant Center, Feinberg School of Medicine, Northwestern University, Chicago, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinayak","family":"Rohan","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Organ Transplant, Feinberg School of Medicine, Northwestern University, Chicago, IL"},{"name":"Comprehensive Transplant Center, Feinberg School of Medicine, Northwestern University, Chicago, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Caldwell","sequence":"additional","affiliation":[{"name":"Chemistry of Life Processes Institute, Northwestern University, Evanston, IL"},{"name":"Proteomics Center of Excellence, Northwestern University, Evanston, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora","family":"Forte","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine, College of Medicine, University of Illinois Chicago, Chicago, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aniel","family":"Sanchez","sequence":"additional","affiliation":[{"name":"Proteomics Center of Excellence, Northwestern University, Evanston, IL"},{"name":"Department of Chemistry, Northwestern University, Evanston, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8815-3372","authenticated-orcid":false,"given":"Neil L.","family":"Kelleher","sequence":"additional","affiliation":[{"name":"Chemistry of Life Processes Institute, Northwestern University, Evanston, IL"},{"name":"Proteomics Center of Excellence, Northwestern University, Evanston, IL"},{"name":"Department of Chemistry, Northwestern University, Evanston, IL"},{"name":"Department of Molecular Biosciences, Northwestern University, Evanston, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satish N.","family":"Nadig","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Organ Transplant, Feinberg School of Medicine, Northwestern University, Chicago, IL"},{"name":"Comprehensive Transplant Center, Feinberg School of Medicine, Northwestern University, Chicago, IL"},{"name":"Department of Microbiology-Immunology and Pediatrics, Feinberg School of Medicine, Northwestern University, Chicago, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026020606460212000_2026.02.02.26345404v1.1","unstructured":"Data & Calculators | HRSA. https:\/\/www.hrsa.gov\/optn\/data-calculators."},{"key":"2026020606460212000_2026.02.02.26345404v1.2","doi-asserted-by":"crossref","first-page":"S22","DOI":"10.1016\/j.ajt.2025.01.020","article-title":"OPTN\/SRTR 2023 Annual Data Report: Kidney","volume":"25","year":"2025","journal-title":"American Journal of Transplantation"},{"key":"2026020606460212000_2026.02.02.26345404v1.3","unstructured":"Naik, R. 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