{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:27:39Z","timestamp":1785421659553,"version":"3.56.0"},"posted":{"date-parts":[[2026,2,5]]},"group-title":"Bioengineering","reference-count":49,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T00:00:00Z","timestamp":1770249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"NIH","award":["T32GM145735"],"award-info":[{"award-number":["T32GM145735"]}]},{"name":"NIH","award":["EY031710"],"award-info":[{"award-number":["EY031710"]}]},{"name":"NIH","award":["R01EY030871"],"award-info":[{"award-number":["R01EY030871"]}]},{"name":"NIH","award":["R21EY035468"],"award-info":[{"award-number":["R21EY035468"]}]},{"name":"NIH","award":["P30EY006360"],"award-info":[{"award-number":["P30EY006360"]}]},{"name":"NIH","award":["R01EY028608"],"award-info":[{"award-number":["R01EY028608"]}]},{"DOI":"10.13039\/100008065","name":"Georgia Research Alliance","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100008065","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/04drjs621","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000879","name":"Alfred P. Sloan Foundation","doi-asserted-by":"crossref","award":["G-2019-11435"],"award-info":[{"award-number":["G-2019-11435"]}],"id":[{"id":"10.13039\/100000879","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,2,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:sec>\n                  <jats:title>Purpose<\/jats:title>\n                  <jats:p>Elevated intraocular pressure (IOP) due to increased outflow resistance through the trabecular meshwork (TM) is a major risk factor for primary open-angle glaucoma. Outflow through the TM is segmental, consisting of high flow (HF) and low flow (LF) regions. Here, we investigate how ocular hypertension impacts segmental outflow using a dexamethasone (DEX) mouse model and compare TM stiffness between HF and LF regions.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>\n                    Nanoparticles containing DEX or vehicle were injected twice weekly in 2\u20134-month-old C57BL\/6J mice (n=14), and IOP was measured weekly. At week 4, mouse eyes were perfused\n                    <jats:italic>in vivo<\/jats:italic>\n                    with fluorescent nanospheres to assess flow patterns and the circumferential percentage of high, intermediate, and low flow regions in each eye. Sagittal sections were collected from HF and LF regions, and atomic force microscopy (AFM) was used to measure tissue stiffness. Immunofluorescent labeling was used to compare fibronectin and \u03b1-SMA protein levels.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>DEX treatment significantly elevated IOP by an average of 33.3% and altered tracer distribution but not the percentage of HF and LF regions around the circumference. No significant differences in TM stiffness were detected between DEX-treated and control mice, or between HF and LF regions. Increased fibronectin in LF regions of DEX-treated eyes suggested subtle TM structural changes that were not detected by AFM.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Dexamethasone alters segmental flow distribution and may impact cell contractility rather than ECM stiffness to cause IOP elevation in young mice. These findings better characterize the nature of segmental outflow and TM mechanics in this model of steroid-induced glaucoma.<\/jats:p>\n                <\/jats:sec>","DOI":"10.64898\/2026.02.03.703547","type":"posted-content","created":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T09:09:07Z","timestamp":1770368947000},"source":"Crossref","is-referenced-by-count":0,"title":["Segmental outflow and trabecular meshwork stiffness in an ocular hypertensive mouse model"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9381-5847","authenticated-orcid":false,"given":"Cydney A.","family":"Wong","sequence":"first","affiliation":[{"name":"Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA"},{"name":"Department of Ophthalmology, Emory University, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. Thomas","family":"Read","sequence":"additional","affiliation":[{"name":"Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guorong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Duke University, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amia","family":"Loveless","sequence":"additional","affiliation":[{"name":"Department of Psychology, Spelman College, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6148-0429","authenticated-orcid":false,"given":"Nina Sara","family":"Fraticelli-Guzm\u00e1n","sequence":"additional","affiliation":[{"name":"George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Feola","sequence":"additional","affiliation":[{"name":"Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA"},{"name":"Department of Ophthalmology, Emory University, Atlanta, Georgia, USA"},{"name":"Center for Visual and Neurocognitive Rehabilitation, Atlanta VA Medical Center Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd","family":"Sulchek","sequence":"additional","affiliation":[{"name":"Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. 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