{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:28:26Z","timestamp":1770748106959,"version":"3.49.0"},"posted":{"date-parts":[[2026,2,5]]},"group-title":"Immunology","reference-count":43,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T00:00:00Z","timestamp":1770249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,2,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Resident memory T cells (T\n                  <jats:sub>RM<\/jats:sub>\n                  ) mediate localized immunity in barrier tissues while central memory T cells (T\n                  <jats:sub>CM<\/jats:sub>\n                  ) recirculate through lymphoid organs to surveil for reinfection. Although T\n                  <jats:sub>RM<\/jats:sub>\n                  are classically associated with peripheral non-lymphoid tissues, they have also been identified within lymph nodes (LN\n                  <jats:sub>RM<\/jats:sub>\n                  ) where the mechanisms guiding their formation and functional differences remain poorly understood.\n                <\/jats:p>\n                <jats:p>\n                  Here we used longitudinal antibody labeling to track the migratory history of memory T cells after influenza infection and demonstrate that CD69\n                  <jats:sup>+<\/jats:sup>\n                  CD103\n                  <jats:sup>+<\/jats:sup>\n                  T cells are resident in the lymph node. LN\n                  <jats:sub>RM<\/jats:sub>\n                  accumulate evenly throughout the lung-draining lymph node and are present within all analyzed LN compartments including, the sub capsular sinus, T cell zone and germinal centers. Epigenetic and transcriptional profiling reveal that LN\n                  <jats:sub>RM<\/jats:sub>\n                  are uniquely poised for cytotoxicity whereas T\n                  <jats:sub>RM<\/jats:sub>\n                  in the lung (Lung\n                  <jats:sub>RM<\/jats:sub>\n                  ) resemble exhausted cells with elevated expression of inhibitory receptors and increased chromatin accessibility at the Pdcd1 locus. Regulatory network analysis of transcription factors, combined with target gene expression and chromatin accessibility, identified key regulons differentiating T\n                  <jats:sub>CM<\/jats:sub>\n                  , LN\n                  <jats:sub>RM<\/jats:sub>\n                  and Lung\n                  <jats:sub>RM<\/jats:sub>\n                  states. Upon antigen re-encounter, LN\n                  <jats:sub>RM<\/jats:sub>\n                  are more proliferative, cytotoxic, and produce more IFN\u03b3 compared to Lung\n                  <jats:sub>RM<\/jats:sub>\n                  . Notably, we find that LN\n                  <jats:sub>RM<\/jats:sub>\n                  represent the most prevalent subset of memory T cells in human thoracic lymph nodes. These findings highlight functional heterogeneity in T\n                  <jats:sub>RM<\/jats:sub>\n                  and establish LN\n                  <jats:sub>RM<\/jats:sub>\n                  as a distinct and durable memory T cell population bridging features of circulating and tissue-resident cells.\n                <\/jats:p>","DOI":"10.64898\/2026.02.03.703569","type":"posted-content","created":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T07:15:13Z","timestamp":1770362113000},"source":"Crossref","is-referenced-by-count":0,"title":["Lymph node resident memory T cells retain effector capabilities by evading lung resident memory dysfunction"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4128-5754","authenticated-orcid":false,"given":"Taylor A","family":"Heim","sequence":"first","affiliation":[{"name":"Koch Institute for Integrative Cancer Research, Massachusetts Institute for Technology, Cambridge, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zachary J","family":"Rogers","sequence":"additional","affiliation":[{"name":"Koch Institute for Integrative Cancer Research, Massachusetts Institute for Technology, Cambridge, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-9178-5604","authenticated-orcid":false,"given":"Sarah","family":"Duquette","sequence":"additional","affiliation":[{"name":"Koch Institute for Integrative Cancer Research, Massachusetts Institute for Technology, Cambridge, MA, USA"},{"name":"Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Molly Y","family":"Carney","sequence":"additional","affiliation":[{"name":"Koch Institute for Integrative Cancer Research, Massachusetts Institute for Technology, Cambridge, MA, USA"},{"name":"Department of Biology, Massachusetts Institute for Technology, Cambridge, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6526-4360","authenticated-orcid":false,"given":"Vidit","family":"Bhandarkar","sequence":"additional","affiliation":[{"name":"Koch Institute for Integrative Cancer Research, Massachusetts Institute for Technology, Cambridge, MA, USA"},{"name":"Department of Biology, Massachusetts Institute for Technology, Cambridge, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2511-9353","authenticated-orcid":false,"given":"Fiona","family":"Chatterjee","sequence":"additional","affiliation":[{"name":"Koch Institute for Integrative Cancer Research, Massachusetts Institute for Technology, Cambridge, MA, USA"},{"name":"Department of Biology, Massachusetts Institute for Technology, Cambridge, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4764-501X","authenticated-orcid":false,"given":"Teresa","family":"Bhandarkar","sequence":"additional","affiliation":[{"name":"Koch Institute for Integrative Cancer Research, Massachusetts Institute for Technology, Cambridge, MA, USA"},{"name":"Department of Biology, Massachusetts Institute for Technology, Cambridge, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4641-6872","authenticated-orcid":false,"given":"Jason M","family":"Schenkel","sequence":"additional","affiliation":[{"name":"Department of Immunology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA"},{"name":"Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA"},{"name":"Department of Chemical Engineering, MIT, Cambridge, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott","family":"Manalis","sequence":"additional","affiliation":[{"name":"Koch Institute for Integrative Cancer Research, Massachusetts Institute for Technology, Cambridge, MA, USA"},{"name":"Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J Christopher","family":"Love","sequence":"additional","affiliation":[{"name":"Koch Institute for Integrative Cancer Research, Massachusetts Institute for Technology, Cambridge, MA, USA"},{"name":"Department of Chemical Engineering, MIT, Cambridge, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3257-4546","authenticated-orcid":false,"given":"Stefani","family":"Spranger","sequence":"additional","affiliation":[{"name":"Koch Institute for Integrative Cancer Research, Massachusetts Institute for Technology, Cambridge, 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