{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:04:40Z","timestamp":1785434680772,"version":"3.56.0"},"posted":{"date-parts":[[2026,2,8]]},"group-title":"Cell Biology","reference-count":50,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T00:00:00Z","timestamp":1770508800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["R35GM136348"],"award-info":[{"award-number":["R35GM136348"]}],"id":[{"id":"https:\/\/ror.org\/04q48ey07","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","award":["T32HD007470"],"award-info":[{"award-number":["T32HD007470"]}]},{"name":"Bakar Aging Research Institute","award":["NA"],"award-info":[{"award-number":["NA"]}]},{"DOI":"10.13039\/100000001","name":"U.S. National Science Foundation","doi-asserted-by":"crossref","award":["2445150"],"award-info":[{"award-number":["2445150"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,2,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  The ovary is one of the first organs to lose functionality with age. We found that aging of the\n                  <jats:italic>Drosophila<\/jats:italic>\n                  ovary is characterized by an accumulation of phenotypes in the somatic compartment, including failure of the follicle cells to encapsulate germ-cell cysts, an extended S phase, and increased DNA damage. In aged ovaries, follicle encapsulation defects are associated with the lack of a germ-cell cyst checkpoint in early oogenesis. Single-cell RNA sequencing revealed that, across all cell types in the ovary, cells in the follicle lineage have the highest number of differentially expressed genes. Overexpression of\n                  <jats:italic>Atg8a<\/jats:italic>\n                  , a key autophagy machinery gene homologous to mammalian LC3, specifically in follicle cells prevents age-associated decline in the follicle epithelium and loss of reproductive capacity. Collectively, these findings demonstrate that genetic manipulation of a small population of ovarian somatic cells is sufficient to improve both cell-autonomous and non-autonomous features of reproductive aging.\n                <\/jats:p>","DOI":"10.64898\/2026.02.05.704044","type":"posted-content","created":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T03:50:12Z","timestamp":1770609012000},"source":"Crossref","is-referenced-by-count":0,"title":["A decline in follicle cell function is a major driver of\n                  <i>Drosophila<\/i>\n                  ovarian aging"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1073-7981","authenticated-orcid":false,"given":"Emily A.","family":"Wolfgram","sequence":"first","affiliation":[{"name":"UCSF, Department of Anatomy"},{"name":"UCSF, Bakar Aging Research Institute"},{"name":"UCSF, Broad Center of Regeneration Medicine and Stem Cell Research"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6250-2394","authenticated-orcid":false,"given":"Todd G.","family":"Nystul","sequence":"additional","affiliation":[{"name":"UCSF, Department of Anatomy"},{"name":"UCSF, Department of OB-GYN\/RS"},{"name":"UCSF, Bakar Aging Research Institute"},{"name":"UCSF, Broad Center of Regeneration Medicine and Stem Cell Research"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026021206402152000_2026.02.05.704044v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.05.039"},{"key":"2026021206402152000_2026.02.05.704044v1.2","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1055\/s-0037-1599086","article-title":"Genetics of reproductive aging from gonadal dysgenesis through menopause","volume":"35","year":"2017","journal-title":"Semin Reprod 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