{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:54:47Z","timestamp":1785412487531,"version":"3.56.0"},"posted":{"date-parts":[[2026,2,11]]},"group-title":"Cell Biology","reference-count":53,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:00:00Z","timestamp":1770768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["514894665"],"award-info":[{"award-number":["514894665"]}],"id":[{"id":"https:\/\/ror.org\/018mejw64","id-type":"ROR","asserted-by":"publisher"}]},{"name":"NIH \/ NCI","award":["P30CA0088748"],"award-info":[{"award-number":["P30CA0088748"]}]},{"name":"NIH \/ NCI","award":["P30CA013696"],"award-info":[{"award-number":["P30CA013696"]}]},{"name":"NIH Shared Instrumentation Grants","award":["S10OD012351"],"award-info":[{"award-number":["S10OD012351"]}]},{"name":"NIH Shared Instrumentation Grants","award":["S10OD021764"],"award-info":[{"award-number":["S10OD021764"]}]},{"name":"NIH Shared Instrumentation Grants","award":["S10OD032433"],"award-info":[{"award-number":["S10OD032433"]}]},{"name":"NIH","award":["5P30DK132710"],"award-info":[{"award-number":["5P30DK132710"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,2,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Ferroptosis is a form of regulated cell death that is characterized by iron-dependent lipid peroxidation. This process is regulated by specific metabolites, the lipid composition of the cells, redox-active iron, and antioxidant mechanisms. Although numerous regulators have been identified over the past decade, exploring other mechanisms, particularly from non-coding genomic regions, can build a thorough understanding of the multifaceted regulatory processes underlying ferroptosis. MicroRNAs (miRNAs) play a crucial role in gene regulation and cellular functions. Through a CRISPR KO screen, we identified miR-940 as a negative regulator of ferroptosis. Overexpression of miR-940 in several cell lines consistently suppressed ferroptosis induced by system x\n                  <jats:sub>c<\/jats:sub>\n                  <jats:sup>-<\/jats:sup>\n                  inhibition. Notably, multiple cancer patient cohorts with elevated miR-940 levels exhibit reduced survival. Integrated bioinformatic, transcriptomic, and proteomic analyses revealed that miR-940 decreases the expression of ACSL4, LPCAT3, DMT1, and NCOA4, and simultaneously increases levels of GPX4. Pharmacological inhibition of GPX4 attenuated the protective effect of miR-940, indicating that its primary anti-ferroptotic activity is mediated through GPX4. Overall, this gene rewiring is associated with reduced levels of redox-active iron and diminished lipid peroxidation, consistent with ferroptosis suppression. These findings suggest that miR-940 coordinates ferroptosis inhibition, which presents a novel regulatory layer for therapeutic exploration in susceptible cancers.\n                <\/jats:p>","DOI":"10.64898\/2026.02.09.704912","type":"posted-content","created":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T16:47:05Z","timestamp":1770828425000},"source":"Crossref","is-referenced-by-count":0,"title":["miR-940 suppresses ferroptosis by controlling expression of key regulatory genes"],"prefix":"10.64898","author":[{"given":"Andrea","family":"Kolak","sequence":"first","affiliation":[{"name":"Cell Signaling and Chemical Biology, Research Unit Signaling and Translation, Helmholtz Zentrum M\u00fcnchen, Neuherberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliane","family":"Tschuck","sequence":"additional","affiliation":[{"name":"Cell Signaling and Chemical Biology, Research Unit Signaling and Translation, Helmholtz Zentrum M\u00fcnchen, Neuherberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanie A. 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