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Correlative light and electron microscopy (CLEM) methods have been developed to preserve biological samples to withstand the harsh environments necessary for electron microscopy. After first being imaged using widefield (WF) and super-resolution structured illumination fluorescence microscopy (SIM), a NanoSuit chemical treatment was applied to a mammalian testis sample before imaging with scanning electron microscopy (SEM). This was done to compare the image quality and resolution of each technique. SEM yields higher resolution and offers validation of results from SIM.<\/jats:p>","DOI":"10.64898\/2026.02.09.704937","type":"posted-content","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T00:40:15Z","timestamp":1770856815000},"source":"Crossref","is-referenced-by-count":0,"title":["Correlative scanning electron and super-resolution structured illumination microscopy"],"prefix":"10.64898","author":[{"given":"Joseph R.","family":"Hamiliton","sequence":"first","affiliation":[{"name":"UCCS BioFrontiers Center, University of Colorado Colorado Springs, 1420 Austin Bluffs Parkway, Colorado Springs, Colorado, 80918, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Summer K.","family":"Levis","sequence":"additional","affiliation":[{"name":"UCCS BioFrontiers Center, University of Colorado Colorado Springs, 1420 Austin Bluffs Parkway, Colorado Springs, Colorado, 80918, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4802-9481","authenticated-orcid":false,"given":"Guy M.","family":"Hagen","sequence":"additional","affiliation":[{"name":"UCCS BioFrontiers Center, University of Colorado Colorado Springs, 1420 Austin Bluffs Parkway, Colorado Springs, Colorado, 80918, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026021400354306000_2026.02.09.704937v1.1","doi-asserted-by":"crossref","unstructured":"Y. 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