{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T03:58:07Z","timestamp":1780459087928,"version":"3.54.1"},"posted":{"date-parts":[[2026,2,13]]},"group-title":"Bioengineering","reference-count":134,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"name":"NSF-CAREER","award":["1651385"],"award-info":[{"award-number":["1651385"]}]},{"name":"NSF CBET","award":["1805157"],"award-info":[{"award-number":["1805157"]}]},{"name":"NIH","award":["1 R01 HL141909-01A1"],"award-info":[{"award-number":["1 R01 HL141909-01A1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,2,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Cardiovascular disease (CVD) is the leading cause of death in the United States and worldwide. While most of these deaths are the result of chronic heart diseases, some CVDs are induced artificially. Doxorubicin (DOX) is a chemotherapeutic that is commonly used to treat breast cancer which is one of the most common types of cancer in the United States. While DOX is an effective anti-cancer agent, over 10% of treated women show signs of acute cardiotoxicity immediately following treatment, and approximately 2% develop severe cardiotoxicity up to 10 years after the end of treatment. Despite this prevalence, the mechanism by which the onset of this cardiotoxicity occurs over time is not well understood. Here, we show that treatment of cardiac cells with DOX changes the cardiac function and the resulting paracrine signaling profile. Subsequent exposure of healthy cells to these altered paracrine agents can recapitulate the effects of direct DOX exposure in 2D and 3D\n                  <jats:italic>in vitro<\/jats:italic>\n                  models. We suggest that this is the result of an altered paracrine miRNA profile and other paracrine factors that propagate the initial disruption caused by direct DOX exposure. Plasma EV miRNA profiling of blinded patient samples revealed distinct clustering by DOX-cardiotoxicity risk, with high-risk patients exhibiting miRNA signatures similar to those from DOX-treated tissue-engineered models. Pathway analysis of the most distinguishing miRNAs linked them to cardiac homeostasis and cardiotoxicity-related mechanisms, supporting the potential of plasma EV miRNAs as noninvasive biomarkers for early risk stratification and personalized cardioprotective interventions in oncological care, and the targeting of key clusters of miRNAs to enhance both understanding of and intervention strategies for preventing the onset of DOX cardiotoxicity.\n                <\/jats:p>","DOI":"10.64898\/2026.02.11.705398","type":"posted-content","created":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T08:05:13Z","timestamp":1771056313000},"source":"Crossref","is-referenced-by-count":1,"title":["Doxorubicin-induced Cardiotoxicity is Propagated by Paracrine Signaling through Small Extracellular Vesicles"],"prefix":"10.64898","author":[{"given":"George","family":"Ronan","sequence":"first","affiliation":[{"name":"Bioengineering Graduate Program, University of Notre Dame, Notre Dame, IN, 46556, USA"},{"name":"Department of Aerospace and Mechanical Engineering, University of Notre Dame, Notre Dame, IN, 46556, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara Ece","family":"Celebi","sequence":"additional","affiliation":[{"name":"Bioengineering Graduate Program, University of Notre Dame, Notre Dame, IN, 46556, USA"},{"name":"Department of Aerospace and Mechanical Engineering, University of Notre Dame, Notre Dame, IN, 46556, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Kowalczyk","sequence":"additional","affiliation":[{"name":"Department of Aerospace and Mechanical Engineering, University of Notre Dame, Notre Dame, IN, 46556, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noor","family":"Behnam","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN, 46556, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Yang","sequence":"additional","affiliation":[{"name":"Bioengineering Graduate Program, University of Notre Dame, Notre Dame, IN, 46556, USA"},{"name":"Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN, 46556, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8079-7663","authenticated-orcid":false,"given":"Lauren","family":"Hawthorne","sequence":"additional","affiliation":[{"name":"Bioengineering Graduate Program, University of Notre Dame, Notre Dame, IN, 46556, USA"},{"name":"Department of Aerospace and Mechanical Engineering, University of Notre Dame, Notre Dame, IN, 46556, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Ketchum","sequence":"additional","affiliation":[{"name":"Bioengineering Graduate Program, University of Notre Dame, Notre Dame, IN, 46556, USA"},{"name":"Department of Aerospace and Mechanical Engineering, University of Notre Dame, Notre Dame, IN, 46556, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aoife J.","family":"Lowery","sequence":"additional","affiliation":[{"name":"Discipline of Surgery, Lambe Institute for Translational Research, University of Galway, Galway, H91 V4AY, IRL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Kerin","sequence":"additional","affiliation":[{"name":"Discipline of Surgery, Lambe Institute for Translational Research, University of Galway, Galway, H91 V4AY, IRL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pinar","family":"Zorlutuna","sequence":"additional","affiliation":[{"name":"Bioengineering Graduate Program, University of Notre Dame, Notre Dame, IN, 46556, USA"},{"name":"Department of Aerospace and Mechanical Engineering, University of Notre Dame, Notre Dame, IN, 46556, USA"},{"name":"Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN, 46556, USA"},{"name":"Harper Cancer Research Institute, University of Notre Dame, Notre Dame, 46556, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026021610100981000_2026.02.11.705398v1.1","first-page":"e93","article-title":"Heart Disease and Stroke Statistics-2023 Update: A Report From the American Heart Association","volume":"147","year":"2023","journal-title":"Circulation"},{"key":"2026021610100981000_2026.02.11.705398v1.2","unstructured":"Olvera Lopez, E. , Ballard, B. 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