{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T14:04:04Z","timestamp":1771855444068,"version":"3.50.1"},"posted":{"date-parts":[[2026,2,18]]},"group-title":"Genetics","reference-count":33,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T00:00:00Z","timestamp":1771372800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,2,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Non-obstructive azoospermia (NOA) is the absence of sperm in the ejaculate due to spermatogenic failure. Fifty percent of NOA cases are unexplained but may arise from unidentified genetic mutations. Variants in\n                  <jats:italic>TEX11<\/jats:italic>\n                  have been identified in men with NOA; and\n                  <jats:italic>Tex11<\/jats:italic>\n                  knockout in mice causes NOA. Here we attempt to validate three\n                  <jats:italic>TEX11<\/jats:italic>\n                  variants discovered in NOA patients by knocking them into the orthologous region of the mouse genome using CRISPR\/Cas9 gene editing. Compared to wild type (144.2 \u00b1 9.87 mg; 1.7 \u00b1 0.5 million sperm\/cauda epididymis 4.8 \u00b1 1.3 pups\/breeding),\n                  <jats:italic>Tex11D<\/jats:italic>\n                  mice (frameshift mutation) had reduced testis weight (28.33 \u00b1 1.16 mg); no sperm in the epididymis; and were infertile with a maturation arrest testicular phenotype. We did not observe any spermatogenesis or fertility defects\n                  <jats:italic>Tex11A<\/jats:italic>\n                  mice (missense mutation).\n                  <jats:italic>Tex11L<\/jats:italic>\n                  mice had reduced testis weight (87.5 \u00b1 14.79 mg) and epididymal sperm counts (0.33\u00b10.13 million\/cauda epididymis) but an incompletely penetrant infertility phenotype (5.4 \u00b1 1.13 pups\/breeding) with one third of mice being infertile. Infertile\n                  <jats:italic>Tex11L<\/jats:italic>\n                  mice also had a distinct epididymal phenotype with reduced sperm density in the caput and no sperm in the cauda, which was filled with amorphous material.\n                <\/jats:p>","DOI":"10.64898\/2026.02.17.706385","type":"posted-content","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T08:05:13Z","timestamp":1771488313000},"source":"Crossref","is-referenced-by-count":0,"title":["<i>Tex11<\/i>\n                  Mutant Mouse Models of Human Azoospermia"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7808-7914","authenticated-orcid":false,"given":"Georgia Rae","family":"Atkins","sequence":"first","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States"},{"name":"Molecular Genetics and Developmental Biology PhD Program, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2562-5898","authenticated-orcid":false,"given":"Rachel L.","family":"Hvasta-Gloria","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5438-7421","authenticated-orcid":false,"given":"Chatchanan","family":"Ausavarungnirun","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States"},{"name":"Molecular Genetics and Developmental Biology PhD Program, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0555-6123","authenticated-orcid":false,"given":"Christopher R.","family":"Pombar","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States"},{"name":"Human Genetics PhD Program, University of Pittsburgh School of Public Health, Pittsburgh, PA, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9292-3894","authenticated-orcid":false,"given":"Jimmaline J.","family":"Hardy","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meena","family":"Sukhwani","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2531-2433","authenticated-orcid":false,"given":"Emily P.","family":"Barnard","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nijole","family":"Pollock","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Malizio","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Sheng","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8806-0918","authenticated-orcid":false,"given":"Miguel A.","family":"Brie\u00f1o-Enr\u00edquez","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Castro","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9644-771X","authenticated-orcid":false,"given":"Tianjiao","family":"Chu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1690-0172","authenticated-orcid":false,"given":"Alexander 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