{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T18:26:16Z","timestamp":1793643976596,"version":"4.5.1"},"posted":{"date-parts":[[2026,2,18]]},"group-title":"Microbiology","reference-count":56,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T00:00:00Z","timestamp":1771372800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"National Institute of Health","award":["R01GM141025"],"award-info":[{"award-number":["R01GM141025"]}]},{"name":"National Institute of Health","award":["R01AI155489"],"award-info":[{"award-number":["R01AI155489"]}]},{"award":["DRG 2459-22"],"award-info":[{"award-number":["DRG 2459-22"]}],"id":[{"id":"https:\/\/ror.org\/01gd7b947","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,2,18]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Intracellular pathogens must navigate the crowded cellular environment to establish infection.\n                  <jats:italic>Listeria monocytogenes<\/jats:italic>\n                  achieves this by recruiting host factors to its surface to hijack the host actin cytoskeleton for motility, form membrane protrusions, and spread from cell to cell. Although these types of\n                  <jats:italic>Listeria<\/jats:italic>\n                  -host interactions are critical for infection, systematic characterization of this interface has been limited. Here, we implement surface display of the promiscuous biotin ligase split-TurboID to profile host proteins recruited to the surface of\n                  <jats:italic>L. monocytogenes<\/jats:italic>\n                  during intracellular infection. This approach identified the host deubiquitinase CYLD as a protein selectively enriched at the pathogen surface. While CYLD promotes infection by suppressing autophagy and innate immunity in macrophages, how\n                  <jats:italic>L. monocytogenes<\/jats:italic>\n                  recruits and appropriates CYLD function in other cell types has remained unclear. We demonstrate that the E3 ligase RNF213 decorates bacterial poles with M1-linked linear ubiquitin, thereby redirecting CYLD to the bacterial surface. We further show ubiquitin is not sufficient to recruit CYLD but requires the\n                  <jats:italic>L. monocytogenes<\/jats:italic>\n                  secreted effector internalin C (InlC). Despite its presence at the bacterial surface, CYLD does not deubiquitinate bacteria or regulate autophagic bacterial clearance in infected epithelial cells. Instead, CYLD and InlC protect\n                  <jats:italic>L. monocytogenes<\/jats:italic>\n                  from IFN-\u03b3- and RNF213-dependent restriction of cell-to-cell spread. Overall, our work profiling the bacterial surface-host interactome has identified a new mechanism by which InlC spatially reprograms CYLD activity, uncoupling its canonical immune functions to promote cell-to-cell spread in epithelial cells. These findings highlight how\n                  <jats:italic>L. monocytogenes<\/jats:italic>\n                  exploits, a host deubiquitinase, to perform cell-type-specific functions during infection.\n                <\/jats:p>","DOI":"10.64898\/2026.02.17.706400","type":"posted-content","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T04:20:20Z","timestamp":1771474820000},"source":"Crossref","is-referenced-by-count":3,"title":["Proximity labeling of the\n                  <i>Listeria monocytogenes<\/i>\n                  surface reveals pathogen control of a host deubiquitinase"],"prefix":"10.64898","author":[{"given":"Patrick J.","family":"Woida","sequence":"first","affiliation":[{"name":"Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maisie W.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca L.","family":"Lamason","sequence":"additional","affiliation":[{"name":"Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026022021551182000_2026.02.17.706400v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.126"},{"key":"2026022021551182000_2026.02.17.706400v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2016.11.007"},{"key":"2026022021551182000_2026.02.17.706400v1.3","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03639.x"},{"key":"2026022021551182000_2026.02.17.706400v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(99)80337-6"},{"key":"2026022021551182000_2026.02.17.706400v1.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2009.08.006"},{"key":"2026022021551182000_2026.02.17.706400v1.6","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1967"},{"key":"2026022021551182000_2026.02.17.706400v1.7","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0125856"},{"key":"2026022021551182000_2026.02.17.706400v1.8","doi-asserted-by":"publisher","DOI":"10.1038\/nature12566"},{"key":"2026022021551182000_2026.02.17.706400v1.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.11.005"},{"key":"2026022021551182000_2026.02.17.706400v1.10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002168"},{"key":"2026022021551182000_2026.02.17.706400v1.11","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-021-00894-4"},{"key":"2026022021551182000_2026.02.17.706400v1.12","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00911-09"},{"key":"2026022021551182000_2026.02.17.706400v1.13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002856"},{"key":"2026022021551182000_2026.02.17.706400v1.14","unstructured":"Lopez-Jimenez AT , Thery F , Wright K , Painter H , Hoffmeister ST , Jarche L , et al. 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Listeria monocytogenes cell-to-cell spread bypasses nutrient limitation for replicating intracellular bacteria. bioRxiv. 2025.","DOI":"10.1101\/2025.01.31.635960"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2026.02.17.706400","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T05:55:22Z","timestamp":1771653322000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2026.02.17.706400"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,18]]},"references-count":56,"URL":"https:\/\/doi.org\/10.64898\/2026.02.17.706400","relation":{},"subject":[],"published":{"date-parts":[[2026,2,18]]},"subtype":"preprint"}}