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We have shown previously that MR1 traffics through endosomal compartments via soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins, including Syntaxin 4 and vesicle-associated membrane protein (VAMP) 4. Here, we investigate the role of synaptosome-associated proteins (SNAPs), which pair with Syntaxins and VAMPs to form functional SNARE complexes, in MR1-mediated antigen presentation. Among SNAP homologs, we identify that SNAP23 contributes to the presentation of\n                  <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                  (Mtb)-derived antigens and loss of SNAP23 reduces the number of MR1-containing vesicles during infection. In contrast, SNAP25 suppresses MR1 presentation for both intracellular pathogens Mtb and\n                  <jats:italic>Mycobacterium avium<\/jats:italic>\n                  , as well as extracellular pathogen\n                  <jats:italic>Candida albicans<\/jats:italic>\n                  . This study demonstrates opposing roles for SNAP23 and SNAP25 in MR1 antigen presentation to MAIT cells, and extends our understanding of how SNAP family proteins regulate MR1 trafficking.\n                <\/jats:p>","DOI":"10.64898\/2026.02.18.706493","type":"posted-content","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T02:05:19Z","timestamp":1771553119000},"source":"Crossref","is-referenced-by-count":0,"title":["Opposing roles for SNAP23 and SNAP25 in mediating MR1 trafficking and antigen presentation"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6704-4644","authenticated-orcid":false,"given":"Se-Jin","family":"Kim","sequence":"first","affiliation":[{"name":"Division of Pulmonary, Allergy, and Critical Care Medicine, Oregon Health & Science University, Portland, OR 97239, USA"},{"name":"Department of Molecular Microbiology and Immunology, Oregon Health & Science University, Portland, OR 97239, 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