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To address these challenges, we developed IntravChip, a continuously perfused microfluidic platform containing a vascularized primary tumor microenvironment (TME) enabling the observation of TC intravasation, and a downstream chamber to collect intravasated TCs. The IntravChip can support a high TC concentration in the TME while maintaining complete vascular perfusion, which we found was necessary to collect intravasated cells. Using MDA-MB-231 breast TCs, we identified an optimal initial TC seeding density that, by day 9, yields a densely populated TME and 100-440 collected intravasated TCs. We validated the IntravChip across several TC types, showing that MDA-MB-231 and MV3 TCs have the highest intravasation rates while MCF-7 TCs have low intravasation efficiency. We also show that the IntravChip is compatible with super-resolution nano-imaging. Our devices enabled high-quality STORM imaging, which revealed that H3K9me3 nanodomains are significantly differentially distributed in intravasated MDA-MB-231 tumor cells compared to those residing in the TME. Finally, the IntravChip was validated as a platform to test the effects of anti-cancer drugs on tumor cells and on the vasculature. We showed that a 5 \u03bcM concentration of sorafenib reduced intravasation events by 69% without impacting the morphology of the microvascular networks (MVNs), while a 10 \u03bcM concentration led to a significant decrease in vessel diameter. This platform enables quantitative analysis of TC intravasation, collection of intravasated TCs for characterization, and screening of anti-metastatic therapies.\n                <\/jats:p>","DOI":"10.64898\/2026.02.19.706805","type":"posted-content","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T18:55:17Z","timestamp":1771613717000},"source":"Crossref","is-referenced-by-count":2,"title":["IntravChip: a vascularized and perfused microfluidic model of the primary tumor microenvironment to collect intravasated tumor cells"],"prefix":"10.64898","author":[{"given":"Marie","family":"Floryan","sequence":"first","affiliation":[{"name":"Department of Mechanical Engineering, Massachusetts Institute of Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Cordiale","sequence":"additional","affiliation":[{"name":"Department of Electronics, Information and Bioengineering, Politecnico di Milano"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Jensen","sequence":"additional","affiliation":[{"name":"Department of Biological Engineering, Massachusetts Institute of Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biological Engineering, Massachusetts Institute of Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixian","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinayak","family":"Vinayak","sequence":"additional","affiliation":[{"name":"Department of Materials Science and Engineering, University of Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sina","family":"Kheiri","sequence":"additional","affiliation":[{"name":"Department of Biological Engineering, Massachusetts Institute of Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ritu","family":"Raman","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, Massachusetts Institute of Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivek","family":"Shenoy","sequence":"additional","affiliation":[{"name":"Department of Materials Science and Engineering, University of Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Cambria","sequence":"additional","affiliation":[{"name":"Department of Biological Engineering, Massachusetts Institute of Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Kamm","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, Massachusetts Institute of Technology"},{"name":"Department of Biological Engineering, Massachusetts Institute of Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026022310402962000_2026.02.19.706805v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2013.377"},{"key":"2026022310402962000_2026.02.19.706805v1.2","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-15-0012"},{"key":"2026022310402962000_2026.02.19.706805v1.3","first-page":"1627","article-title":"Tumor angiogenesis and its possible role in intravasation of colorectal epithelial cells","volume":"7","year":"2001","journal-title":"Clin. 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