{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T01:04:25Z","timestamp":1771981465528,"version":"3.50.1"},"posted":{"date-parts":[[2026,2,22]]},"group-title":"Cell Biology","reference-count":75,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T00:00:00Z","timestamp":1771718400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,2,22]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Trogocytosis is a form of cellular cannibalism in which a cell \u201cbites\u201d off pieces of another cell. Here, we investigate the molecular mechanisms of a developmentally programmed\n                  <jats:italic>C. elegans<\/jats:italic>\n                  trogocytic event that occurs when endodermal cells bite off and digest pieces of primordial germ cells (PGCs) called lobes. Through a genetic screen, we identify the Rab family small GTPase\n                  <jats:italic>rab-35<\/jats:italic>\n                  as a central regulator of trogocytosis and show that its function is required in both biting (endodermal) and bitten (PGC) cells. Within endodermal cells, RAB-35 enriches around trogocytosed PGC lobes, promotes the removal of phosphatidylinositol 4,5-bisphosphate (PIP\n                  <jats:sub>2<\/jats:sub>\n                  ), and is required for lobe digestion. By contrast, we show that RAB-35 within PGCs works with the ESCRT complex to promote scission of the PGC lobe from the cell body. Our findings identify a new regulator of trogocytosis that has distinct functions in the biting and bitten cells and provide evidence that the bitten cell contributes to the scission of its own membrane.\n                <\/jats:p>","DOI":"10.64898\/2026.02.20.707090","type":"posted-content","created":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T21:10:14Z","timestamp":1771794614000},"source":"Crossref","is-referenced-by-count":0,"title":["RAB-35 regulates distinct steps of trogocytosis in the biting and bitten cell"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0966-6012","authenticated-orcid":false,"given":"Julie","family":"Manikas","sequence":"first","affiliation":[{"name":"Department of Cell Biology, NYU Grossman School of Medicine, New York, NY 10016"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liam","family":"Popovsky","sequence":"additional","affiliation":[{"name":"Center for Quantitative Cell Imaging, U. of Wisconsin, Madison, WI 53706"},{"name":"Department of Cell and Regenerative Biology, U. of Wisconsin, Madison, WI 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4574-4455","authenticated-orcid":false,"given":"Yusuff","family":"Abdu","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, NYU Grossman School of Medicine, New York, NY 10016"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4212-7731","authenticated-orcid":false,"given":"Jeremy","family":"Nance","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, NYU Grossman School of Medicine, New York, NY 10016"},{"name":"Center for Quantitative Cell Imaging, U. of Wisconsin, Madison, WI 53706"},{"name":"Department of Cell and Regenerative Biology, U. of Wisconsin, Madison, WI 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2026022416250910000_2026.02.20.707090v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3439"},{"key":"2026022416250910000_2026.02.20.707090v1.2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201694613"},{"key":"2026022416250910000_2026.02.20.707090v1.3","doi-asserted-by":"publisher","DOI":"10.1242\/dev.115048"},{"key":"2026022416250910000_2026.02.20.707090v1.4","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.2.875"},{"key":"2026022416250910000_2026.02.20.707090v1.5","doi-asserted-by":"crossref","unstructured":"Bettadapur, A. , H.W. 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