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While mouse models of\n                  <jats:italic>Chd8<\/jats:italic>\n                  haploinsufficiency recapitulate brain overgrowth, the specific cellular mechanisms and developmental timing that lead to these anatomical abnormalities remain poorly understood. Here, we conducted 3D imaging of\n                  <jats:italic>Chd8<\/jats:italic>\n                  <jats:sup>V986*\/+<\/jats:sup>\n                  mouse brains using magnetic resonance imaging followed by tissue clearing and cellular resolution light-sheet microscopy across embryonic and postnatal developmental stages. We found that brain overgrowth occurs postnatally, driven by an increase in non-neuronal cells prior to volumetric expansion. Unexpectedly, we identified prevalent molecular layer heterotopias (MLH) within the frontal cortex of\n                  <jats:italic>Chd8<\/jats:italic>\n                  <jats:sup>V986*\/+<\/jats:sup>\n                  mice composed of neurons breaking through the pial surface during embryonic development and persisting throughout life. Increased incidence of MLH, previously identified in individuals with idiopathic autism, was replicated across three independent\n                  <jats:italic>\n                    Chd8\n                    <jats:sup>+\/-<\/jats:sup>\n                  <\/jats:italic>\n                  mouse models and present across multiple genomic backgrounds, establishing aberrant neuronal migration as a core feature of\n                  <jats:italic>Chd8<\/jats:italic>\n                  haploinsufficiency.\n                <\/jats:p>","DOI":"10.64898\/2026.02.28.708624","type":"posted-content","created":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T15:25:19Z","timestamp":1772551519000},"source":"Crossref","is-referenced-by-count":3,"title":["<i>Chd8<\/i>\n                  haploinsufficiency leads to molecular layer heterotopias and age-dependent cortical expansion"],"prefix":"10.64898","author":[{"given":"Felix A","family":"Kyere","sequence":"first","affiliation":[{"name":"UNC Neuroscience Center, University of North Carolina, Chapel Hill, NC, 27599, USA"},{"name":"Department of Genetics, University of North Carolina, Chapel Hill, NC, 27599, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6650-7574","authenticated-orcid":false,"given":"Ian","family":"Curtin","sequence":"additional","affiliation":[{"name":"UNC Neuroscience Center, University of North Carolina, Chapel Hill, NC, 27599, USA"},{"name":"Department of Genetics, University of North Carolina, Chapel Hill, NC, 27599, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziquan","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Computer Science, University of North Carolina, Chapel Hill, NC, 27599, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cesar P","family":"Canales","sequence":"additional","affiliation":[{"name":"UC Davis Center for Neuroscience, Davis, CA 95618, USA"},{"name":"Department of Psychiatry and Behavioral Sciences, University of California Davis, Sacramento, CA 95817, USA"},{"name":"The M.I.N.D. 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