{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:05:34Z","timestamp":1785423934891,"version":"3.56.0"},"posted":{"date-parts":[[2026,3,3]]},"group-title":"Developmental Biology","reference-count":82,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T00:00:00Z","timestamp":1772496000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["R35GM119458"],"award-info":[{"award-number":["R35GM119458"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"id":[{"id":"https:\/\/ror.org\/01hcyya48","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,3,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Tissue-resident macrophages are increasingly recognized for their roles in promoting organogenesis, yet how macrophages are involved in fetal ovarian development remains unclear. In particular, little is known about ovarian macrophage ontogeny and how it relates to germ cell entry into meiosis and establishment of the oocyte reserve. Here we combine temporally-controlled lineage tracing of yolk-sac erythro-myeloid progenitors, fetal HSC-derived progenitors, and postnatal monocytes to map multi-wave seeding and remodeling of ovarian macrophages across fetal and early postnatal life. We identify three major resident subsets defined by MHCII and CSF1R that display distinct expansion kinetics and persistence, and we show that CCR2-dependent monocyte recruitment is required for efficient maturation of postnatal macrophage populations. Functionally, transient or sustained depletion of CSF1R\u207a fetal macrophages perturbs ovarian vascular growth and triggers precocious meiotic initiation without overt loss of germ cells, leading to persistent, premature meiotic progression. Extending macrophage depletion into late gestation disrupts perinatal physiological germ cell attrition despite rapid postnatal macrophage repopulation. Together, our findings establish ovarian macrophages as stage-specific regulators that couple immune ontogeny to ovarian morphogenesis and germ cell quality control during establishment of the oocyte reserve.<\/jats:p>\n                <jats:sec>\n                  <jats:title>One Sentence Summary<\/jats:title>\n                  <jats:p>Ovarian macrophages are required for the proper timing of germ cell meiotic entry and progression, vascular growth, and for the physiological clearance of germ cells during establishment of the oocyte reserve in perinatal stages.<\/jats:p>\n                <\/jats:sec>","DOI":"10.64898\/2026.02.28.708733","type":"posted-content","created":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T17:35:17Z","timestamp":1772559317000},"source":"Crossref","is-referenced-by-count":0,"title":["Macrophages regulate meiotic initiation and germ cell clearance in the developing ovary"],"prefix":"10.64898","author":[{"given":"Xiaowei","family":"Gu","sequence":"first","affiliation":[{"name":"Reproductive Sciences Center, Division of Developmental Biology, Cincinnati Children\u2019s Hospital Medical Center, Cincinnati, OH, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoko","family":"Matsuyama","sequence":"additional","affiliation":[{"name":"Reproductive Sciences Center, Division of Developmental Biology, Cincinnati Children\u2019s Hospital Medical Center, Cincinnati, OH, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-Yun","family":"Li","sequence":"additional","affiliation":[{"name":"Reproductive Sciences Center, Division of Developmental Biology, Cincinnati Children\u2019s Hospital Medical Center, Cincinnati, OH, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4133-7040","authenticated-orcid":false,"given":"Tony","family":"DeFalco","sequence":"additional","affiliation":[{"name":"Reproductive Sciences Center, Division of Developmental Biology, Cincinnati Children\u2019s Hospital Medical Center, Cincinnati, OH, U.S.A."},{"name":"Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026030521053244000_2026.02.28.708733v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2016.02.024"},{"key":"2026030521053244000_2026.02.28.708733v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2016.02.015"},{"key":"2026030521053244000_2026.02.28.708733v1.3","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.add1967"},{"key":"2026030521053244000_2026.02.28.708733v1.4","doi-asserted-by":"publisher","DOI":"10.1111\/febs.15848"},{"key":"2026030521053244000_2026.02.28.708733v1.5","doi-asserted-by":"crossref","first-page":"1282658","DOI":"10.3389\/fendo.2023.1282658","article-title":"New insight into the role of macrophages in ovarian function and ovarian aging","volume":"14","year":"2023","journal-title":"Front Endocrinol 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