{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T21:55:09Z","timestamp":1777758909685,"version":"3.51.4"},"posted":{"date-parts":[[2026,3,5]]},"group-title":"Cancer Biology","reference-count":35,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T00:00:00Z","timestamp":1772668800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"DOI":"10.13039\/100016764","name":"Fred and Pamela Buffett Cancer Center","doi-asserted-by":"crossref","award":["P30CA036727"],"award-info":[{"award-number":["P30CA036727"]}],"id":[{"id":"10.13039\/100016764","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["P20GM121316"],"award-info":[{"award-number":["P20GM121316"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R37CA262635"],"award-info":[{"award-number":["R37CA262635"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01AI53090"],"award-info":[{"award-number":["R01AI53090"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Cancer Institute","award":["P30CA042014"],"award-info":[{"award-number":["P30CA042014"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,4,27]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>Mantle cell lymphoma (MCL) is one of the deadliest forms of Non-Hodgkin\u2019s B-cell lymphoma. Typically, patients present with both overexpression of CyclinD1 and secondary mutations identified by genomic sequencing. Although MCL patients may initially respond to treatment, they eventually relapse and succumb to disease, highlighting the essential need to identify new targets for treatment. Here we performed proteomic profiling of healthy B cells and three different forms of B-cell malignancies, including MCL, to define the proteomic signature of MCL. We compared the proteome of each to MCL and identified 10 proteins that are specifically upregulated in MCL. Of these 10 proteins, seven of them show no transcriptional changes and have been overlooked by conventional RNA expression analysis. Further analysis of the proteomic signature reveals potential avenues for dual targeting in CAR T-cell therapy and provides guidance for personalized therapeutics based on protein expression.<\/jats:p>\n                <jats:sec>\n                  <jats:title>STATEMENT OF SIGNIFICANCE<\/jats:title>\n                  <jats:p>We present a resource defining the protein landscape of MCL, CLL, and FL as compared to healthy b cells identified utilizing quantitative proteomics from primary patient samples. Applied to MCL, our results identify 10 proteins specifically upregulated in MCL that may prove to be therapeutic targets to treat the disease.<\/jats:p>\n                <\/jats:sec>","DOI":"10.64898\/2026.03.02.709116","type":"posted-content","created":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T10:45:35Z","timestamp":1772707535000},"source":"Crossref","is-referenced-by-count":0,"title":["Proteome landscape of B-cell malignancies identifies mantle cell lymphoma protein signature"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5650-3841","authenticated-orcid":false,"given":"Samantha A.","family":"Swenson","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA"},{"name":"Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, NE, USA"},{"name":"Fred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. Bea","family":"Winship","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Hematology & Hematopoietic Malignancies, University of Utah, Salt Lake City, UT USA"},{"name":"Huntsman Cancer Institute, University of Utah, Salt Lake City, UT USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kasidy K.","family":"Dobish","sequence":"additional","affiliation":[{"name":"Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, NE, USA"},{"name":"Fred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE"},{"name":"Department of Internal Medicine, Division of Hematology & Hematopoietic Malignancies, University of Utah, Salt Lake City, UT USA"},{"name":"Huntsman Cancer Institute, University of Utah, Salt Lake City, UT USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karli J.","family":"Wittorf","sequence":"additional","affiliation":[{"name":"Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, NE, USA"},{"name":"Fred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE"},{"name":"Creighton University School of Medicine, Omaha, NE, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry Chun","family":"Hin Law","sequence":"additional","affiliation":[{"name":"Fred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE"},{"name":"Eppley Institute, University of Nebraska Medical Center, Omaha, NE, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie M.","family":"Vose","sequence":"additional","affiliation":[{"name":"Fred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE"},{"name":"Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy","family":"Greiner","sequence":"additional","affiliation":[{"name":"Fred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE"},{"name":"Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6309-9472","authenticated-orcid":false,"given":"Michael R.","family":"Green","sequence":"additional","affiliation":[{"name":"Department of Lymphoma and Myeloma, Division of Cancer Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX, USA"},{"name":"Department of Genomic Medicine, University of Texas MD Anderson Cancer Center, Houston, TX, USA"},{"name":"Center for Cancer Epigenetics, University of Texas MD Anderson Cancer Center, Houston, TX, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4962-9083","authenticated-orcid":false,"given":"Nicholas T.","family":"Woods","sequence":"additional","affiliation":[{"name":"Fred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE"},{"name":"Eppley Institute, University of Nebraska Medical Center, Omaha, NE, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shannon M.","family":"Buckley","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Hematology & Hematopoietic Malignancies, University of Utah, Salt Lake City, UT USA"},{"name":"Huntsman Cancer Institute, University of Utah, Salt Lake City, UT USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2026042906150697000_2026.03.02.709116v2.1","unstructured":"Mak TW , Saunders ME , and Jett BD . Primer to The immune response. 2nd edition \/ ed. Amsterdam ; Boston: Elsevier\/AP Cell, AP Cell is an imprint of Elsevier; 2014. xxvii, 674 pages p."},{"key":"2026042906150697000_2026.03.02.709116v2.2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-01-643569"},{"key":"2026042906150697000_2026.03.02.709116v2.3","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-019-0602-x"},{"key":"2026042906150697000_2026.03.02.709116v2.4","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-10-3411"},{"key":"2026042906150697000_2026.03.02.709116v2.5","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1914347"},{"key":"2026042906150697000_2026.03.02.709116v2.6","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.14122.1"},{"key":"2026042906150697000_2026.03.02.709116v2.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2016.11.005"},{"key":"2026042906150697000_2026.03.02.709116v2.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2010.03.010"},{"key":"2026042906150697000_2026.03.02.709116v2.9","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-10-3999"},{"key":"2026042906150697000_2026.03.02.709116v2.10","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.RA117.000539"},{"key":"2026042906150697000_2026.03.02.709116v2.11","doi-asserted-by":"publisher","DOI":"10.1038\/bcj.2016.80"},{"key":"2026042906150697000_2026.03.02.709116v2.12","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-10-532200"},{"key":"2026042906150697000_2026.03.02.709116v2.13","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2019002102"},{"key":"2026042906150697000_2026.03.02.709116v2.14","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2020.274258"},{"key":"2026042906150697000_2026.03.02.709116v2.15","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-01-478834"},{"key":"2026042906150697000_2026.03.02.709116v2.16","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-021-09061-3"},{"key":"2026042906150697000_2026.03.02.709116v2.17","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14051284"},{"key":"2026042906150697000_2026.03.02.709116v2.18","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfma.2024.04.002"},{"key":"2026042906150697000_2026.03.02.709116v2.19","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3654"},{"key":"2026042906150697000_2026.03.02.709116v2.20","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10010104"},{"key":"2026042906150697000_2026.03.02.709116v2.21","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-021-00555-4"},{"key":"2026042906150697000_2026.03.02.709116v2.22","doi-asserted-by":"publisher","DOI":"10.1593\/neo.121784"},{"key":"2026042906150697000_2026.03.02.709116v2.23","doi-asserted-by":"publisher","DOI":"10.3390\/cimb46030156"},{"key":"2026042906150697000_2026.03.02.709116v2.24","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2013.867486"},{"key":"2026042906150697000_2026.03.02.709116v2.25","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.25696"},{"key":"2026042906150697000_2026.03.02.709116v2.26","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.013359"},{"key":"2026042906150697000_2026.03.02.709116v2.27","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-19-0945"},{"key":"2026042906150697000_2026.03.02.709116v2.28","doi-asserted-by":"publisher","DOI":"10.1038\/nature11468"},{"key":"2026042906150697000_2026.03.02.709116v2.29","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-07-370122"},{"key":"2026042906150697000_2026.03.02.709116v2.30","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.17383"},{"key":"2026042906150697000_2026.03.02.709116v2.31","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0014085"},{"issue":"10","key":"2026042906150697000_2026.03.02.709116v2.32","first-page":"3951","article-title":"Inhibition of GST-pi nuclear transfer increases mantle cell lymphoma sensitivity to cisplatin, cytarabine, gemcitabine, bortezomib and doxorubicin","volume":"30","year":"2010","journal-title":"Anticancer Res"},{"key":"2026042906150697000_2026.03.02.709116v2.33","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-024-04093-5"},{"key":"2026042906150697000_2026.03.02.709116v2.34","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-28839-y"},{"key":"2026042906150697000_2026.03.02.709116v2.35","doi-asserted-by":"publisher","DOI":"10.62347\/IMFU7145"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2026.03.02.709116","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T13:16:00Z","timestamp":1777468560000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2026.03.02.709116"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,5]]},"references-count":35,"URL":"https:\/\/doi.org\/10.64898\/2026.03.02.709116","relation":{},"subject":[],"published":{"date-parts":[[2026,3,5]]},"subtype":"preprint"}}