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Because DRG neurons are heterogeneous with overlapping electrophysiological properties, methods to distinguish neuron subtypes are valuable for properly interpreting the measurements and drawing conclusions. Automated patch clamp recording offers an approach for conducting these experiments at higher throughput than conventional recording methods, but identification of neuron subtypes is challenging. We developed a method for recording from acutely isolated mouse DRG neurons using automated patch clamp recording coupled to optogenetic stimulation that was capable of discerning Na\n                  <jats:sub>V<\/jats:sub>\n                  1.8 and TRPV1 expressing neuron subpopulations. This approach can facilitate physiological and pharmacological studies of DRG neurons with potential value in developing and testing targeted analgesic agents.\n                <\/jats:p>","DOI":"10.64898\/2026.03.05.709933","type":"posted-content","created":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T20:00:22Z","timestamp":1773086422000},"source":"Crossref","is-referenced-by-count":0,"title":["Combining automated patch clamp with optogenetics enables selective recording of DRG neurons subtypes"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4935-1122","authenticated-orcid":false,"given":"Carlos G.","family":"Vanoye","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2919-1051","authenticated-orcid":false,"given":"Dongjun","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2989-1938","authenticated-orcid":false,"given":"Abdelhak","family":"Belmadani","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1428-0384","authenticated-orcid":false,"given":"Anne-Marie","family":"Malfait","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Rheumatology, Rush University Medical Center, Chicago, IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard J.","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3993-966X","authenticated-orcid":false,"suffix":"Jr.","given":"Alfred L.","family":"George","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2026031119351459000_2026.03.05.709933v1.1","doi-asserted-by":"crossref","unstructured":"Wood JN , Yan N , Huang J , Zhao J , Akopian A , Cox JJ , Woods CG and Nassar MA . 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