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Genome-wide loss-of-function screens can illuminate single genes\n                  <jats:italic>necessary<\/jats:italic>\n                  for virus entry, but are stymied by genetic redundancy. Here we report a genome-wide CRISPR activation screening strategy to discover single genes that are\n                  <jats:italic>sufficient<\/jats:italic>\n                  for viral entry into normally-uninfectable cells. Sequential rounds of viral infection vastly enhanced screening sensitivity. This sequential screening strategy was generalizable to two unrelated viruses\u2014Ebola and rabies viruses\u2014and could broadly accelerate the discovery of viral entry factors.\n                <\/jats:p>","DOI":"10.64898\/2026.03.06.710083","type":"posted-content","created":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T04:46:08Z","timestamp":1772858768000},"source":"Crossref","is-referenced-by-count":0,"title":["Elucidating genes sufficient for viral entry into cells through sequential genome-wide CRISPR activation screens"],"prefix":"10.64898","author":[{"given":"Timothy","family":"Chai","sequence":"first","affiliation":[{"name":"Institute for Stem Cell Biology & Regenerative Medicine, Department of Developmental Biology, Stanford University, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicia","family":"Wong","sequence":"additional","affiliation":[{"name":"Institute for Stem Cell Biology & Regenerative Medicine, Department of 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