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However, these platform technologies have for the most part failed to translate to broad use in the private sector due to a lack of high-throughput, user-friendly platforms. Here we present an automated high-throughput organ chip seeded with iPSC-derived cardiomyocytes transduced with GCaMP6f and interface with translational technologies to bridge the current academia-industry gap. Cardiomyocytes were seeded on-chip fully hands-free using an entry-level fluid handling robot to significantly reduce user handling requirements. Pipette interfaces were paramount to facilitating seeding and feeding through improved tolerances for establishing a functional connection to dispense and collect small fluidic volumes. Following successful seeding, GCaMP6f activity on-chip was monitored with our automated, non-invasive fiber-optic sensing platform. We show a significant decrease in cardiomyocyte beat rate in response to decreased ambient culture temperature using data collected with our optical sensing platform. This study provides a potential translational blueprint for academia-industry partnership toward broad adoption of organ chip technology in drug development and disease modeling.<\/jats:p>","DOI":"10.64898\/2026.03.11.711145","type":"posted-content","created":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T03:15:18Z","timestamp":1773544518000},"source":"Crossref","is-referenced-by-count":1,"title":["Automated culture and monitoring of a high-throughput human heart-on-a-chip"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8405-429X","authenticated-orcid":false,"given":"Bryan G.","family":"Schellberg","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering, Northeastern University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1603-0995","authenticated-orcid":false,"given":"Nolan T.","family":"Burson","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Northeastern University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Gomes","sequence":"additional","affiliation":[{"name":"Parallel Fluidics, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7346-2685","authenticated-orcid":false,"given":"Guohao","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Northeastern University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0433-9290","authenticated-orcid":false,"given":"Abigail N.","family":"Koppes","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Northeastern University, Boston, Massachusetts, USA"},{"name":"Department of Bioengineering, Northeastern University, Boston, Massachusetts, USA"},{"name":"Department of Biology, Northeastern University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3376-6358","authenticated-orcid":false,"given":"Ryan A.","family":"Koppes","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Northeastern University, Boston, Massachusetts, USA"},{"name":"Department of Bioengineering, Northeastern University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026031704500880000_2026.03.11.711145v1.1","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1038\/s41467-019-13896-7","article-title":"Microengineered human blood\u2013 brain barrier platform for understanding nanoparticle transport mechanisms","volume":"11","year":"2020","journal-title":"Nature communications"},{"key":"2026031704500880000_2026.03.11.711145v1.2","doi-asserted-by":"publisher","DOI":"10.1002\/adbi.202000133"},{"key":"2026031704500880000_2026.03.11.711145v1.3","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201902042"},{"key":"2026031704500880000_2026.03.11.711145v1.4","doi-asserted-by":"publisher","DOI":"10.1126\/science.1188302"},{"key":"2026031704500880000_2026.03.11.711145v1.5","doi-asserted-by":"publisher","DOI":"10.1039\/c2lc40074j"},{"key":"2026031704500880000_2026.03.11.711145v1.6","doi-asserted-by":"crossref","unstructured":"Brown, J. 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