{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T20:55:16Z","timestamp":1773521716765,"version":"3.50.1"},"posted":{"date-parts":[[2026,3,12]]},"group-title":"Microbiology","reference-count":39,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T00:00:00Z","timestamp":1773273600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"name":"NIH","award":["R01EY032482"],"award-info":[{"award-number":["R01EY032482"]}]},{"name":"NIH","award":["U24EY035102"],"award-info":[{"award-number":["U24EY035102"]}]},{"name":"NIH","award":["T32EY017271"],"award-info":[{"award-number":["T32EY017271"]}]},{"name":"NIH","award":["EY036686"],"award-info":[{"award-number":["EY036686"]}]},{"name":"CORE Grant","award":["P30 EY08098"],"award-info":[{"award-number":["P30 EY08098"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,3,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  The ocular surface is a mucosal tissue that is constantly exposed to environmental antigens and potential pathogens. Human microbiomes play a critical role in the balance of surveillance and inflammation at sites of colonization. Historically, the investigation of the ocular microbiome has been difficult due to its paucibacterial nature and the inhospitable environment of the ocular surface. Despite this,\n                  <jats:italic>Corynebacterium mastitidis<\/jats:italic>\n                  (\n                  <jats:italic>C. mast<\/jats:italic>\n                  ) developed a unique ability to colonize the eye and elicit a protective immune response characterized by induction of IL-17 from \u03b3\u03b4 T cells and protection from corneal infection. Therefore, we sought to understand the unique bacterial machinery that\n                  <jats:italic>C. mast<\/jats:italic>\n                  utilizes to colonize the eye and how it affects the induction of an eye-specific immune signature. Using a\n                  <jats:italic>C. mast<\/jats:italic>\n                  transposon mutant library, we identified a mutant that completely lacked an ability to form biofilm, colonize the eye, and induce\n                  <jats:italic>in vivo<\/jats:italic>\n                  immunity. Whole genome sequencing revealed a disruption in the sortase F gene, which anchors proteins to the cell wall of\n                  <jats:italic>C. mast<\/jats:italic>\n                  , governing biofilm formation and tethering of adhesins to the cell surface. Additionally, we show that mutation in individual\n                  <jats:italic>C. mast<\/jats:italic>\n                  adhesins does not affect ocular colonization or immune induction. By understanding the molecular mechanism of ocular microbial colonization, this work advances our understanding of how bacteria colonize and induce immune responses on the eye, providing a foundation for developing novel therapeutic strategies against ocular infections.\n                <\/jats:p>","DOI":"10.64898\/2026.03.12.711320","type":"posted-content","created":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T04:20:16Z","timestamp":1773375616000},"source":"Crossref","is-referenced-by-count":0,"title":["<i>C. mastitidis<\/i>\n                  requires the protein Sortase F to colonize the eye"],"prefix":"10.64898","author":[{"given":"Yannis E.","family":"Rigas","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA 15219"},{"name":"Department of Immunology, University of Pittsburgh, Pittsburgh, PA 15219"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jackie","family":"Shane","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA 15219"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin","family":"Treat","sequence":"additional","affiliation":[{"name":"Infectious Diseases, St. Jude Children\u2019s Research Hospital, Memphis, TN 38105"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert M.Q.","family":"Shanks","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA 15219"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony J. 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