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Here, we developed monoclonal antibodies against marmoset CG\u03b1 and CG\u03b2, and established a non-invasive immunochromatographic CG assay. By eliminating invasive blood sampling, this assay supports 3Rs principles and enables practical endocrine monitoring. The assay detected urinary CG surges preceding ovulation, enabling efficient embryo recovery through artificial insemination (75%). Early pregnancy was detected at approximately 17 days post-ovulation. In addition, pregnancy detection in squirrel monkeys suggests conservation of CG features among certain New World primates. Overall, this simple, non-invasive assay provides a practical tool for marmoset research and establishes a foundation for future conservation-oriented reproductive monitoring following appropriate species-specific validation.<\/jats:p>","DOI":"10.64898\/2026.03.12.711492","type":"posted-content","created":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T17:15:21Z","timestamp":1773681321000},"source":"Crossref","is-referenced-by-count":0,"title":["A chorionic gonadotropin assay enables non-invasive detection of ovulation and early pregnancy in a New World primate model"],"prefix":"10.64898","author":[{"given":"Keiko","family":"Kishimoto","sequence":"first","affiliation":[{"name":"Division of Advanced Physiology, Central Institute for Experimental Medicine and Life Science (CIEM), 3-25-12 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, 216-0821, 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