{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:01:34Z","timestamp":1785427294456,"version":"3.56.0"},"posted":{"date-parts":[[2026,3,17]]},"group-title":"Developmental Biology","reference-count":38,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T00:00:00Z","timestamp":1773705600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["R00HD098298"],"award-info":[{"award-number":["R00HD098298"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["DP2NS131787"],"award-info":[{"award-number":["DP2NS131787"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,3,17]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Cell type identity in the nervous system is encoded within cis-regulatory landscapes that integrate transcription factor activity with chromatin accessibility. However, how these regulatory programs are organized and remodeled during post-embryonic neural development is poorly understood. We generate a temporally resolved single-cell chromatin accessibility atlas of \u223c95,000 zebrafish brain and retina nuclei spanning larval, juvenile, and adult stages. We define 212 discrete chromatin states and uncover widespread, cell type-specific chromatin reorganization across development. By integrating with transcriptomic data, we link motif accessibility to transcription factor expression and identify regulatory programs that are either maintained or reconfigured during post-embryonic development of each neural cell type. Leveraging this atlas, we systematically identify and functionally validate candidate enhancers in vivo. Focusing on the\n                  <jats:italic>slc1a3b<\/jats:italic>\n                  locus in radial glia, we define evolutionarily conserved, compact enhancer modules that act combinatorially to drive gene expression. Together, these findings provide a systems-level framework for decoding neural regulatory logic and enable functional dissection of conserved cis-regulatory programs in the vertebrate nervous system.\n                <\/jats:p>","DOI":"10.64898\/2026.03.16.712178","type":"posted-content","created":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T17:05:42Z","timestamp":1773767142000},"source":"Crossref","is-referenced-by-count":1,"title":["Single-cell chromatin profiling reveals dynamic regulatory logic and enhancer elements in brain and retina development"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0255-2717","authenticated-orcid":false,"given":"Jessie E.","family":"Greenslade","sequence":"first","affiliation":[{"name":"Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 19104, 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