{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T22:28:22Z","timestamp":1787783302981,"version":"build-2784847793"},"posted":{"date-parts":[[2026,3,18]]},"group-title":"Developmental Biology","reference-count":31,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"name":"NIH\/NIDDK","award":["P30DK127984"],"award-info":[{"award-number":["P30DK127984"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,3,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Purine nucleotides are essential for mammalian development\n                  <jats:sup>1,2<\/jats:sup>\n                  . Purine monophosphates support cell signaling and proliferation and are synthesized by cells through either de novo synthesis or a salvage pathway\n                  <jats:sup>3<\/jats:sup>\n                  . We previously identified a midgestational metabolic transition in mice (gestational days gd10.5\u201311.5) characterized by changes in purine metabolism\n                  <jats:sup>4<\/jats:sup>\n                  . Midgestation is a period of rapid growth for placenta and embryo, yet it remains unclear how the placental tissues expand without directly competing with the embryo for biosynthetic resources. Here, we show that this midgestational metabolic transition is associated with a marked reduction in embryonic expression of purine salvage enzymes, which constrains embryonic metabolism and leads to different strategies for purine synthesis between the placenta and embryo. Midgestation embryos are unable to engage the purine salvage pathway even when de novo purine synthesis is blocked either in vivo or in ex utero embryo culture, whereas placental tissue and trophoblasts retain the capacity to use either pathway. Disruption of de novo purine synthesis in mice causes reduced embryonic growth, impaired axial elongation, and abnormal brain and placental development, which are only partially rescued by supplementation with purine salvage precursors. In human placenta, trophoblast stem cells readily switch between the de novo and salvage pathways based on nutrient availability, and syncytiotrophoblasts (STB) preferentially rely on the salvage pathway. We identified guanosine monophosphate (GMP) as a metabolic checkpoint regulating STB differentiation, with insufficient GMP levels causing degradation of the small GTPase Rheb and failure of mTOR activation. Supplementation of purine salvage substrates restored GMP synthesis and STB differentiation in humans, but not mice. Further, in vivo measurements in humans revealed that maternal circulating hypoxanthine decreases during pregnancy and is further reduced in women with clinically small placentas, highlighting the role of hypoxanthine in supporting placental growth. These results uncover compartmentalized purine salvage between the embryo and placenta as a mechanism that limits competition for biosynthetic resources and enables coordinated growth during mammalian development.\n                <\/jats:p>","DOI":"10.64898\/2026.03.18.712680","type":"posted-content","created":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T09:25:14Z","timestamp":1774257914000},"source":"Crossref","is-referenced-by-count":1,"title":["Midgestation metabolic constraint in purine metabolism drives distinct strategies for placenta and fetal growth"],"prefix":"10.64898","author":[{"given":"Weizhi","family":"Xu","sequence":"first","affiliation":[{"name":"Laboratory of Developmental Metabolism and Placental Biology, Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, Texas, 75390, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy","family":"De La Cruz","sequence":"additional","affiliation":[{"name":"Laboratory of Developmental Metabolism and Placental Biology, Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, Texas, 75390, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Woods","sequence":"additional","affiliation":[{"name":"Laboratory of Developmental Metabolism and Placental Biology, Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, Texas, 75390, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry","family":"Lokshtanov","sequence":"additional","affiliation":[{"name":"Janelia Research Campus, Howard Hughes Medical Institute, Ashburn Virginia, 20147, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shihong","family":"Gao","sequence":"additional","affiliation":[{"name":"Janelia Research Campus, Howard 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USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald D.","family":"McIntire","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine L","family":"Duryea","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA"},{"name":"Parkland Health, Dallas, TX 75235, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David B.","family":"Nelson","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA"},{"name":"Parkland Health, Dallas, TX 75235, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine 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