{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T16:33:26Z","timestamp":1793118806473,"version":"4.5.0"},"posted":{"date-parts":[[2026,3,23]]},"group-title":"Neuroscience","reference-count":45,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:00:00Z","timestamp":1774224000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"award":["R01HD114146"],"award-info":[{"award-number":["R01HD114146"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"name":"American Diabetes Association","award":["1-24-PDF-56"],"award-info":[{"award-number":["1-24-PDF-56"]}],"id":[{"id":"https:\/\/ror.org\/04f6cgz95","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Silver Endowment","id":[{"id":"https:\/\/ror.org\/0229evh14","id-type":"ROR","asserted-by":"crossref"}]},{"name":"NIHR Senior Investigator Award"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,10,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Male parenting behavior is highly conserved and activated only after offspring are born. Here we show that the behavioral switch from infanticide in virgin male mice to caregiving in wild-type sires is associated with enhanced expression and activation of Transient receptor potential channel 5 (Trpc5) in estrogen receptor \u03b1 (Esr1)-expressing neurons in the hypothalamic medial preoptic area (MPOA). While selective deletion of Trpc5 from Esr1\n                  <jats:sup>MPOA<\/jats:sup>\n                  neurons diminishes paternal behavior in sires, overexpression of Trpc5 in these neurons converts the otherwise infanticidal virgin males to exhibit care to pups. Mechanistically, Trpc5-dependent changes in neuronal excitability underlie fatherhood-associated Esr1\n                  <jats:sup>MPOA<\/jats:sup>\n                  neuron plasticity. Notably, Trpc5 overexpression also enhances escape behavior and elicits exploratory diving behavior, suggesting that these neurons control a broader adaptive parenting response in males. These findings establish a Trpc5-dependent MPOA neural signal as a critical regulator of paternal behavior.\n                <\/jats:p>","DOI":"10.64898\/2026.03.20.713203","type":"posted-content","created":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T19:10:14Z","timestamp":1774293014000},"source":"Crossref","is-referenced-by-count":1,"title":["Paternal behavior is controlled by preoptic Trpc5 neurons"],"prefix":"10.64898","author":[{"given":"Yongxiang","family":"Li","sequence":"first","affiliation":[{"name":"USDA\/ARS Children\u2019s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL 33613, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingzhuo","family":"Liu","sequence":"additional","affiliation":[{"name":"USDA\/ARS Children\u2019s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL 33613, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuhui","family":"Wang","sequence":"additional","affiliation":[{"name":"USDA\/ARS Children\u2019s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL 33613, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristine M.","family":"McDermott","sequence":"additional","affiliation":[{"name":"USDA\/ARS Children\u2019s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL 33613, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengjie","family":"Wang","sequence":"additional","affiliation":[{"name":"USDA\/ARS Children\u2019s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL 33613, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Deng","sequence":"additional","affiliation":[{"name":"USDA\/ARS Children\u2019s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL 33613, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxue","family":"Yang","sequence":"additional","affiliation":[{"name":"USDA\/ARS Children\u2019s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL 33613, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutian","family":"Liu","sequence":"additional","affiliation":[{"name":"USDA\/ARS Children\u2019s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL 33613, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Cheng","sequence":"additional","affiliation":[{"name":"USDA\/ARS Children\u2019s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL 33613, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meixin","family":"Sun","sequence":"additional","affiliation":[{"name":"USDA\/ARS Children\u2019s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL 33613, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinming","family":"Liu","sequence":"additional","affiliation":[{"name":"USDA\/ARS Children\u2019s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL 33613, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinjing","family":"Jian","sequence":"additional","affiliation":[{"name":"USDA\/ARS Children\u2019s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL 33613, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiamin","family":"Qiu","sequence":"additional","affiliation":[{"name":"USDA\/ARS Children\u2019s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL 33613, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Wu","sequence":"additional","affiliation":[{"name":"USDA\/ARS Children\u2019s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL 33613, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lamei","family":"Xue","sequence":"additional","affiliation":[{"name":"USDA\/ARS Children\u2019s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL 33613, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Zhou","sequence":"additional","affiliation":[{"name":"USDA\/ARS Children\u2019s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL 33613, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjie","family":"Yang","sequence":"additional","affiliation":[{"name":"USDA\/ARS Children\u2019s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL 33613, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailan","family":"Liu","sequence":"additional","affiliation":[{"name":"USDA\/ARS Children\u2019s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL 33613, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longlong","family":"Tu","sequence":"additional","affiliation":[{"name":"USDA\/ARS Children\u2019s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL 33613, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin R.","family":"Arenkiel","sequence":"additional","affiliation":[{"name":"Jan and Dan Duncan Neurological Research Institute, Texas Children\u2019s Hospital, Department of Pediatrics, Section of Neurology, Baylor College of Medicine, Houston, Texas, 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I. Sadaf","family":"Farooqi","sequence":"additional","affiliation":[{"name":"University of Cambridge Metabolic Research Laboratories; Institute of Metabolic Science and NIHR Cambridge Biomedical Research Centre, Cambridge, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Xu","sequence":"additional","affiliation":[{"name":"USDA\/ARS Children\u2019s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL 33613, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026102709050841000_2026.03.20.713203v2.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2015.04.004"},{"key":"2026102709050841000_2026.03.20.713203v2.2","doi-asserted-by":"publisher","DOI":"10.1037\/0021-843X.109.4.588"},{"key":"2026102709050841000_2026.03.20.713203v2.3","doi-asserted-by":"publisher","DOI":"10.1177\/1559827619849028"},{"key":"2026102709050841000_2026.03.20.713203v2.4","doi-asserted-by":"publisher","DOI":"10.2307\/2136804"},{"key":"2026102709050841000_2026.03.20.713203v2.5","doi-asserted-by":"publisher","DOI":"10.1159\/000092677"},{"key":"2026102709050841000_2026.03.20.713203v2.6","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2016.0140"},{"key":"2026102709050841000_2026.03.20.713203v2.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2019.05.008"},{"key":"2026102709050841000_2026.03.20.713203v2.8","doi-asserted-by":"publisher","DOI":"10.1126\/science.1253291"},{"key":"2026102709050841000_2026.03.20.713203v2.9","doi-asserted-by":"publisher","DOI":"10.1126\/science.1252723"},{"key":"2026102709050841000_2026.03.20.713203v2.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2018.02.002"},{"key":"2026102709050841000_2026.03.20.713203v2.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2022.03.033"},{"key":"2026102709050841000_2026.03.20.713203v2.12","doi-asserted-by":"publisher","DOI":"10.1037\/0735-7044.116.6.968"},{"key":"2026102709050841000_2026.03.20.713203v2.13","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0027-0"},{"key":"2026102709050841000_2026.03.20.713203v2.14","doi-asserted-by":"publisher","DOI":"10.1038\/nature13307"},{"key":"2026102709050841000_2026.03.20.713203v2.15","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.109204"},{"key":"2026102709050841000_2026.03.20.713203v2.16","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-025-09268-5"},{"key":"2026102709050841000_2026.03.20.713203v2.17","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.07.007"},{"key":"2026102709050841000_2026.03.20.713203v2.18","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-020-00784-3"},{"key":"2026102709050841000_2026.03.20.713203v2.19","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2018.02.019"},{"key":"2026102709050841000_2026.03.20.713203v2.20","doi-asserted-by":"publisher","DOI":"10.1006\/hbeh.1997.1430"},{"key":"2026102709050841000_2026.03.20.713203v2.21","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0558-22.2022"},{"key":"2026102709050841000_2026.03.20.713203v2.22","doi-asserted-by":"publisher","DOI":"10.1038\/nature02196"},{"key":"2026102709050841000_2026.03.20.713203v2.23","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.06.001"},{"key":"2026102709050841000_2026.03.20.713203v2.24","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.11.032"},{"key":"2026102709050841000_2026.03.20.713203v2.25","doi-asserted-by":"publisher","DOI":"10.1126\/science.adi0576"},{"key":"2026102709050841000_2026.03.20.713203v2.26","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2021.03.033"},{"key":"2026102709050841000_2026.03.20.713203v2.27","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.112771"},{"key":"2026102709050841000_2026.03.20.713203v2.28","doi-asserted-by":"publisher","DOI":"10.1007\/7854_2017_11"},{"key":"2026102709050841000_2026.03.20.713203v2.29","doi-asserted-by":"publisher","DOI":"10.1002\/dev.21070"},{"key":"2026102709050841000_2026.03.20.713203v2.30","doi-asserted-by":"publisher","DOI":"10.1016\/j.yfrne.2014.11.007"},{"key":"2026102709050841000_2026.03.20.713203v2.31","doi-asserted-by":"publisher","DOI":"10.1016\/S0149-7634(03)00010-1"},{"key":"2026102709050841000_2026.03.20.713203v2.32","doi-asserted-by":"publisher","DOI":"10.1080\/19424620.2015.1082805"},{"key":"2026102709050841000_2026.03.20.713203v2.33","doi-asserted-by":"publisher","DOI":"10.1111\/cars.12356"},{"key":"2026102709050841000_2026.03.20.713203v2.34","doi-asserted-by":"publisher","DOI":"10.1016\/j.earlhumdev.2016.07.001"},{"key":"2026102709050841000_2026.03.20.713203v2.35","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2024.05.006"},{"key":"2026102709050841000_2026.03.20.713203v2.36","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2004.06.019"},{"key":"2026102709050841000_2026.03.20.713203v2.37","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2018.10.037"},{"key":"2026102709050841000_2026.03.20.713203v2.38","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-54215-2_6"},{"key":"2026102709050841000_2026.03.20.713203v2.39","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201696369"},{"key":"2026102709050841000_2026.03.20.713203v2.40","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M504745200"},{"key":"2026102709050841000_2026.03.20.713203v2.41","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-005-1419-1"},{"key":"2026102709050841000_2026.03.20.713203v2.42","doi-asserted-by":"publisher","DOI":"10.1534\/g3.115.020982"},{"key":"2026102709050841000_2026.03.20.713203v2.43","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12212-7"},{"key":"2026102709050841000_2026.03.20.713203v2.44","doi-asserted-by":"publisher","DOI":"10.1093\/eep\/dvy026"},{"key":"2026102709050841000_2026.03.20.713203v2.45","doi-asserted-by":"publisher","DOI":"10.1038\/s41380-019-0506-1"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2026.03.20.713203","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T16:05:21Z","timestamp":1793117121000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2026.03.20.713203"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,23]]},"references-count":45,"URL":"https:\/\/doi.org\/10.64898\/2026.03.20.713203","relation":{},"subject":[],"published":{"date-parts":[[2026,3,23]]},"subtype":"preprint"}}